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The map label for this gene is artI [H]

Identifier: 170748044

GI number: 170748044

Start: 1737371

End: 1738354

Strand: Reverse

Name: artI [H]

Synonym: Mrad2831_1626

Alternate gene names: 170748044

Gene position: 1738354-1737371 (Counterclockwise)

Preceding gene: 170748051

Following gene: 170748043

Centisome position: 28.6

GC content: 73.98

Gene sequence:

>984_bases
GTGAGCCAGTCCCCGCCCCCGCGCTCCGAGAGGAGCACCACCCCGTCCCAGTCGTCAAGAGACGATAGGACAGTCATCCC
ATCCCGGATCGCGGCGGCCGCGGTCGCGGCACTCCTCGCGGGGCCGGCCGGCGCCGCGGCGCCCCCGGCCGCGCCCGCCC
CTGCAGCTGCCCCTGCCGCCGCCACCGCCGGGGCCGCGGGCGGGACCGCGACCCCCGCCGTCGCGGTGCCGGATTTCTGG
AACCCGCGCAGCCGGGGCGAGCGGATCGAGGCGCCGCAGACCGGCCGGGCGGTCCGCTTCCTCACGGACGACGAGTTCCC
GCCGCTGCACTTCGCCGGCCCCGACGGCAACCCGACGGGCTTCGTGGTCGAGCTCGCCCGGGCCGTCTGCGAGAAGCTGA
CGATCACCTGCACGGTGCAGGCGCGCCGCTTCGACACCCTGCTCGACGCCCTCGACGGCAAGCAGGGCGACGTGGTCGCG
GCCGCCATCCCGCTGACCGCGCCCCTGCGGTCGAAATTCCTGGCGACGCGGCCGTATTTCCGCTGGCCGGCGCGCTTCGC
GGCCCGGACCGACAAGGGCCTGCCGGTCCCCTCGGCGGCGGCGCTCGCCGGCCGCAGCGTCGGGGTCATCGCCGGCAGCG
CCCACGCGGCCTACCTGAAGGCGTTCTTCCCGAAGGCCGTGCCCAAGGACTTCACAGACCTCACGGCGGCCGAGAGCGCG
CTCAAGCGCGGCGAGATCGACTACCTGTTCGCCGACGGCCTGAACCTCGCCCTCTATGTCGGCGGCCAGGACGCGGAGAA
TTGCTGCGCGCTGACCGGCGGCGACTACCTGGAGAACCGCTATTTCGGGGAGGGGATCGGCCTGATCACCCGCCAGGAGG
ACGCGGCCCTGTCCCGGGCGCTGGACGACGCGCTCCAGCGGGTCTGGGACGACGGCAAGTACACCGAGCTGTATCTGCGG
TTCTTCCCGGTGAGCCCCTTCTGA

Upstream 100 bases:

>100_bases
AGGCGATCTCCGGCGGCAGGGCCTCCGGCCCCGCGGGCGGGCGCTGGAACGACACGCCGCGCGCTCCCCCGGTGGCCCCA
GGACGTGGTAGAGGAGGGGG

Downstream 100 bases:

>100_bases
GGGCTTTTATCGCGCGCGGGGATCCTGCCCGCCGGGCTTCGGCTGCCGGACGGCACCATGACGGACCTGCTCGGGAAGGC
CGCGGGCGGCGCGCGGCCTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSQSPPPRSERSTTPSQSSRDDRTVIPSRIAAAAVAALLAGPAGAAAPPAAPAPAAAPAAATAGAAGGTATPAVAVPDFW
NPRSRGERIEAPQTGRAVRFLTDDEFPPLHFAGPDGNPTGFVVELARAVCEKLTITCTVQARRFDTLLDALDGKQGDVVA
AAIPLTAPLRSKFLATRPYFRWPARFAARTDKGLPVPSAAALAGRSVGVIAGSAHAAYLKAFFPKAVPKDFTDLTAAESA
LKRGEIDYLFADGLNLALYVGGQDAENCCALTGGDYLENRYFGEGIGLITRQEDAALSRALDDALQRVWDDGKYTELYLR
FFPVSPF

Sequences:

>Translated_327_residues
MSQSPPPRSERSTTPSQSSRDDRTVIPSRIAAAAVAALLAGPAGAAAPPAAPAPAAAPAAATAGAAGGTATPAVAVPDFW
NPRSRGERIEAPQTGRAVRFLTDDEFPPLHFAGPDGNPTGFVVELARAVCEKLTITCTVQARRFDTLLDALDGKQGDVVA
AAIPLTAPLRSKFLATRPYFRWPARFAARTDKGLPVPSAAALAGRSVGVIAGSAHAAYLKAFFPKAVPKDFTDLTAAESA
LKRGEIDYLFADGLNLALYVGGQDAENCCALTGGDYLENRYFGEGIGLITRQEDAALSRALDDALQRVWDDGKYTELYLR
FFPVSPF
>Mature_326_residues
SQSPPPRSERSTTPSQSSRDDRTVIPSRIAAAAVAALLAGPAGAAAPPAAPAPAAAPAAATAGAAGGTATPAVAVPDFWN
PRSRGERIEAPQTGRAVRFLTDDEFPPLHFAGPDGNPTGFVVELARAVCEKLTITCTVQARRFDTLLDALDGKQGDVVAA
AIPLTAPLRSKFLATRPYFRWPARFAARTDKGLPVPSAAALAGRSVGVIAGSAHAAYLKAFFPKAVPKDFTDLTAAESAL
KRGEIDYLFADGLNLALYVGGQDAENCCALTGGDYLENRYFGEGIGLITRQEDAALSRALDDALQRVWDDGKYTELYLRF
FPVSPF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=28.3842794759825, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788649, Length=233, Percent_Identity=26.6094420600858, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788648, Length=241, Percent_Identity=23.2365145228216, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788228, Length=217, Percent_Identity=25.3456221198157, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 34374; Mature: 34243

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSPPPRSERSTTPSQSSRDDRTVIPSRIAAAAVAALLAGPAGAAAPPAAPAPAAAPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
ATAGAAGGTATPAVAVPDFWNPRSRGERIEAPQTGRAVRFLTDDEFPPLHFAGPDGNPTG
HHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCH
FVVELARAVCEKLTITCTVQARRFDTLLDALDGKQGDVVAAAIPLTAPLRSKFLATRPYF
HHHHHHHHHHHCEEEEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCH
RWPARFAARTDKGLPVPSAAALAGRSVGVIAGSAHAAYLKAFFPKAVPKDFTDLTAAESA
HCCHHHHHCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LKRGEIDYLFADGLNLALYVGGQDAENCCALTGGDYLENRYFGEGIGLITRQEDAALSRA
HHCCCCEEEEECCCEEEEEECCCCCCCEEEECCCHHHHCCCCCCCCCEEEECHHHHHHHH
LDDALQRVWDDGKYTELYLRFFPVSPF
HHHHHHHHHCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
SQSPPPRSERSTTPSQSSRDDRTVIPSRIAAAAVAALLAGPAGAAAPPAAPAPAAAPAA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
ATAGAAGGTATPAVAVPDFWNPRSRGERIEAPQTGRAVRFLTDDEFPPLHFAGPDGNPTG
HHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCH
FVVELARAVCEKLTITCTVQARRFDTLLDALDGKQGDVVAAAIPLTAPLRSKFLATRPYF
HHHHHHHHHHHCEEEEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCH
RWPARFAARTDKGLPVPSAAALAGRSVGVIAGSAHAAYLKAFFPKAVPKDFTDLTAAESA
HCCHHHHHCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LKRGEIDYLFADGLNLALYVGGQDAENCCALTGGDYLENRYFGEGIGLITRQEDAALSRA
HHCCCCEEEEECCCEEEEEECCCCCCCEEEECCCHHHHCCCCCCCCCEEEECHHHHHHHH
LDDALQRVWDDGKYTELYLRFFPVSPF
HHHHHHHHHCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]