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The map label for this gene is cobD [H]

Identifier: 170747893

GI number: 170747893

Start: 1540279

End: 1541274

Strand: Reverse

Name: cobD [H]

Synonym: Mrad2831_1475

Alternate gene names: 170747893

Gene position: 1541274-1540279 (Counterclockwise)

Preceding gene: 170747895

Following gene: 170747892

Centisome position: 25.36

GC content: 78.31

Gene sequence:

>996_bases
ATGGTCCATCTGCCCGGTACGTTTGCCGACACGCTCGCCGTCCTCGCCGTCGCCCTCGGGATCGAGGCGGCGGCCGGCTA
TCCCGACGCGCTCTACAGGGCGGCCGGTCACCCTGTCACCTGGATCGGCCGGCTGATCGCCGCCCTGGAGCGCGGTCTCA
ACCGCGGCTCGGCGCGCCGGCGGCGGGCCGCCGGCTGCCTCGCGCTGGCGCTGCTCCTCGCCGCGGTCGGCTCGGCCGCC
CTCGCCGTCGCGGCGCTGGCGGGGCTCGCCGGCCCCCTCCCCGCCCTGCTCCTCCTCGGCCTGATCTGCGCCAGCCTGCC
GGCGCAGCGCAGCCTGCACCAGCACGTCGCCGCCGTGGAGACGGCCCTGCGCGCGGGCGGCCTGGAGGCGGGGCGCCGGG
CCGTGGCGATGATCGTCGGGCGCGACCCGGACCGCCTCGACGAGGCGGCGGTCTGCCGGGCCGCGATCGAGAGCCTGGCG
GAGAACTTCTCCGACGGGATCGTGGCGCCGGCCTTCTGGATCGGCTGCCTCGGCCTGCCGGGCGGCGCCCTCTACAAGGC
GATCAACACCGCCGACAGCATGATCGGCCACCGCAGCCCGCGCTACGAGGCCTTCGGCTGGGCGGCCGCGCGGCTCGACG
ACGGCGTGAACCTGCCGGCCTCCCGGCTCGCGGGCCTGCTGATCGCCGGGGCGGCGGCCCTGCGGGGCGCCGACGCCCGC
GGGGCGCTCCGGGCGATGGCCCGGGACGCGCGCCGGCACCGCTCGCCGAATGCCGGCTGGCCCGAATCCGCCATGGCCGG
CGCGCTGGGCCTGCGGCTCGCGGGGCCGCGGGTCTACGACGGCACCCTCGTCCCGGACGCCCATATGGGCGACGGCCGGG
ACGCGGCCGCGGCCGACGACATCGCCCGGGCCCTGACCCTCTACCGGACGGCCTGCTGGCTCCAGGGCGGGCTCGTGCTG
GCGCTCCTCGGGCTCTGCCTGACCCTGGCGGGCTGA

Upstream 100 bases:

>100_bases
GCCAGGGCTCCGGCGCGTCGGGAAAGGCTTGTCGGAGTCCGCCGAGATCGCCGCCATGCGCGATCGGGGCGACCTCACGC
GCGGGAACGCTCCACACCGA

Downstream 100 bases:

>100_bases
GCCGTCTTCGCTCCGCGCGCCGGGACGGCGGCATCCCCCGATCGGGCCTTGGCACGGCCCGCGCACCGGACGCATCGTGG
CCGGACGGGGCCGGACGGGG

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MVHLPGTFADTLAVLAVALGIEAAAGYPDALYRAAGHPVTWIGRLIAALERGLNRGSARRRRAAGCLALALLLAAVGSAA
LAVAALAGLAGPLPALLLLGLICASLPAQRSLHQHVAAVETALRAGGLEAGRRAVAMIVGRDPDRLDEAAVCRAAIESLA
ENFSDGIVAPAFWIGCLGLPGGALYKAINTADSMIGHRSPRYEAFGWAAARLDDGVNLPASRLAGLLIAGAAALRGADAR
GALRAMARDARRHRSPNAGWPESAMAGALGLRLAGPRVYDGTLVPDAHMGDGRDAAAADDIARALTLYRTACWLQGGLVL
ALLGLCLTLAG

Sequences:

>Translated_331_residues
MVHLPGTFADTLAVLAVALGIEAAAGYPDALYRAAGHPVTWIGRLIAALERGLNRGSARRRRAAGCLALALLLAAVGSAA
LAVAALAGLAGPLPALLLLGLICASLPAQRSLHQHVAAVETALRAGGLEAGRRAVAMIVGRDPDRLDEAAVCRAAIESLA
ENFSDGIVAPAFWIGCLGLPGGALYKAINTADSMIGHRSPRYEAFGWAAARLDDGVNLPASRLAGLLIAGAAALRGADAR
GALRAMARDARRHRSPNAGWPESAMAGALGLRLAGPRVYDGTLVPDAHMGDGRDAAAADDIARALTLYRTACWLQGGLVL
ALLGLCLTLAG
>Mature_331_residues
MVHLPGTFADTLAVLAVALGIEAAAGYPDALYRAAGHPVTWIGRLIAALERGLNRGSARRRRAAGCLALALLLAAVGSAA
LAVAALAGLAGPLPALLLLGLICASLPAQRSLHQHVAAVETALRAGGLEAGRRAVAMIVGRDPDRLDEAAVCRAAIESLA
ENFSDGIVAPAFWIGCLGLPGGALYKAINTADSMIGHRSPRYEAFGWAAARLDDGVNLPASRLAGLLIAGAAALRGADAR
GALRAMARDARRHRSPNAGWPESAMAGALGLRLAGPRVYDGTLVPDAHMGDGRDAAAADDIARALTLYRTACWLQGGLVL
ALLGLCLTLAG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33778; Mature: 33778

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHLPGTFADTLAVLAVALGIEAAAGYPDALYRAAGHPVTWIGRLIAALERGLNRGSARR
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHH
RRAAGCLALALLLAAVGSAALAVAALAGLAGPLPALLLLGLICASLPAQRSLHQHVAAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TALRAGGLEAGRRAVAMIVGRDPDRLDEAAVCRAAIESLAENFSDGIVAPAFWIGCLGLP
HHHHHCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
GGALYKAINTADSMIGHRSPRYEAFGWAAARLDDGVNLPASRLAGLLIAGAAALRGADAR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHH
GALRAMARDARRHRSPNAGWPESAMAGALGLRLAGPRVYDGTLVPDAHMGDGRDAAAADD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHH
IARALTLYRTACWLQGGLVLALLGLCLTLAG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVHLPGTFADTLAVLAVALGIEAAAGYPDALYRAAGHPVTWIGRLIAALERGLNRGSARR
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHH
RRAAGCLALALLLAAVGSAALAVAALAGLAGPLPALLLLGLICASLPAQRSLHQHVAAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TALRAGGLEAGRRAVAMIVGRDPDRLDEAAVCRAAIESLAENFSDGIVAPAFWIGCLGLP
HHHHHCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
GGALYKAINTADSMIGHRSPRYEAFGWAAARLDDGVNLPASRLAGLLIAGAAALRGADAR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHH
GALRAMARDARRHRSPNAGWPESAMAGALGLRLAGPRVYDGTLVPDAHMGDGRDAAAADD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHH
IARALTLYRTACWLQGGLVLALLGLCLTLAG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]