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The map label for this gene is rpfC [H]

Identifier: 170747868

GI number: 170747868

Start: 1514604

End: 1516250

Strand: Reverse

Name: rpfC [H]

Synonym: Mrad2831_1450

Alternate gene names: 170747868

Gene position: 1516250-1514604 (Counterclockwise)

Preceding gene: 170747869

Following gene: 170747860

Centisome position: 24.95

GC content: 72.5

Gene sequence:

>1647_bases
ATGTTCGGGTCGCGGCGCCGGCGGGACGAGGCGTACGACAACGAGAAGGCGCAGGCGCTCATCCGGGCCGTCGTCATCGG
CCTCGCCTGCCTGTACCTCCTGCCCCAGATCTACGGCGGGATCGCCCCGCCGGAGGCCCGGTTCGTCGCCTACCTCGTGA
CGTGCCACTGGGCCTTCGCCGTGGCGCTGTACGGCTGGATCACCCGGAATCCCGGCAGCAACACGCCGCGCCGCGTCGTC
ACCTTCGTCCTCGACCTCGGCGGGATGACCTACACGATGGCGGTGGGCGGCGCGCCGTTCCTGCCGCTCTACGCCATCGT
GATCCGGCTGATCGTCGGCAACGGCCTGCGCTACGGGGTGACCGCCCTGAAGGCGTCCACGGCCGCGGCCCTGGTCTCGA
TCGCGATCACCACGTACTGCAACGCGTACTGGCGCGCGAACCCGTTCCTCGTCCTGACGCTGACGGCCATCACCGTCCTC
GTGCCGACCTACATCTACAGCCTGCTGGAGCGCCTGCGCGGCGCCTACGAGCGCGAGCAGGAGGCGAGCCTGTCCAAGTC
GCGCTTCCTGGCACAGGCGAGCCACGATCTGCGCCAGCCGATCCACGCCATCAGCCTGTTCACCGCCTGCCTGCGCGGCG
CCAGGCTGGGGCCGGAGGAGCTGCAGATGGTCGAGAACATCGACCGGTCGCTGCAGAGCGTCTCGCGCCTGTTCAAGTCG
CTCCTGGACATCTCGACCCTCGACAGCGGCCGGGTGGAGCCCCGGCCCGAGCCCGTCGCGATCGACGACGTCATCGAGGA
TGTGCGGCGCCAGAACTCGGAGGCCGCCCAGATCGCCGGGACGGCTCTGCGCAGCCGCGCCTGCGCGGTCGTGGTGCGCA
CCGACCGGGCACTGCTCACGACGATGCTCCAGAACATCGTCAACAACGCGATCAAGTACGCCCCCGGTCGGCCGATCCTG
ATCGCCTGCCGCCGGCGGGGCGACCGGCTCGCCGTCCAGGTCTACGATCGCGGCCTCGGAATCCCGGTCGCGCATCAGGA
GCGGGTCTTCGACGAGTTCTACCAGGTCCGCCGCCGCGGCGACCGGGATGTCGAGGGCGTGGGCCTCGGCCTGCCGATCG
TCCGGCGGCTCGGCGAGCTCCTGGACCTCGCGGTGACGCTGCGGTCGGTGCCGGGTCGCGGGACCTGCGTGACGATCGGC
AACCTGCCCCTCGCCCGGGCTCCGGAGCCCGCGGCCCTCCCCGCCGGGCTCGACCCGCCGGCGACGATCGGCGGCCTGCG
CGTGCTGCTGGTGGAGGATGACGGGGCGGTGCTGCGGGCCACGGCCAGCCTGCTGGAGACGTGGGGCTGCCGCGTTCAGC
CCGAGACCGGGATCCCCGACGCCGTTCCCGAGATCGACCTCCTCGTCACCGATTTCGAGCTCGGGAACGGCGTCACCGGG
ACGGACTGCATCGCGGCGGTCCGGCGGCTCGCCGGCCGCGCACTCCCGGCGGTGGTGATGAGCGGCCACGACGAGGCGCG
CGTGCGCGAGGATCTGGGGGCGCCGGACATCCCGATCCTCTCGAAGCCGGTCCGGCCCGCCGAGCTGCGCTCCGTGCTCA
CCGCCGCGAGCCTCCGGCTGCGCCCTCGGCCCGCGCCGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGGCGGGATCCGGGGCGGACGCCGCGGGGACCGTCCCGGCGAGGCGGGCGATCGGCGGCACCGCCGGGCGGCGCGCCGC
CGGCCGGGCCTGAGCCGCCG

Downstream 100 bases:

>100_bases
GACGCGCGGCCGACGCCTCAGCGGGCCGGTTCGGCCGGATCCGTGCCGGGACGCGCGGTCCCCGCTCCGCCGCCCTGCTG
GTCGAGCATCGCCTTGAGCA

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MFGSRRRRDEAYDNEKAQALIRAVVIGLACLYLLPQIYGGIAPPEARFVAYLVTCHWAFAVALYGWITRNPGSNTPRRVV
TFVLDLGGMTYTMAVGGAPFLPLYAIVIRLIVGNGLRYGVTALKASTAAALVSIAITTYCNAYWRANPFLVLTLTAITVL
VPTYIYSLLERLRGAYEREQEASLSKSRFLAQASHDLRQPIHAISLFTACLRGARLGPEELQMVENIDRSLQSVSRLFKS
LLDISTLDSGRVEPRPEPVAIDDVIEDVRRQNSEAAQIAGTALRSRACAVVVRTDRALLTTMLQNIVNNAIKYAPGRPIL
IACRRRGDRLAVQVYDRGLGIPVAHQERVFDEFYQVRRRGDRDVEGVGLGLPIVRRLGELLDLAVTLRSVPGRGTCVTIG
NLPLARAPEPAALPAGLDPPATIGGLRVLLVEDDGAVLRATASLLETWGCRVQPETGIPDAVPEIDLLVTDFELGNGVTG
TDCIAAVRRLAGRALPAVVMSGHDEARVREDLGAPDIPILSKPVRPAELRSVLTAASLRLRPRPAPAA

Sequences:

>Translated_548_residues
MFGSRRRRDEAYDNEKAQALIRAVVIGLACLYLLPQIYGGIAPPEARFVAYLVTCHWAFAVALYGWITRNPGSNTPRRVV
TFVLDLGGMTYTMAVGGAPFLPLYAIVIRLIVGNGLRYGVTALKASTAAALVSIAITTYCNAYWRANPFLVLTLTAITVL
VPTYIYSLLERLRGAYEREQEASLSKSRFLAQASHDLRQPIHAISLFTACLRGARLGPEELQMVENIDRSLQSVSRLFKS
LLDISTLDSGRVEPRPEPVAIDDVIEDVRRQNSEAAQIAGTALRSRACAVVVRTDRALLTTMLQNIVNNAIKYAPGRPIL
IACRRRGDRLAVQVYDRGLGIPVAHQERVFDEFYQVRRRGDRDVEGVGLGLPIVRRLGELLDLAVTLRSVPGRGTCVTIG
NLPLARAPEPAALPAGLDPPATIGGLRVLLVEDDGAVLRATASLLETWGCRVQPETGIPDAVPEIDLLVTDFELGNGVTG
TDCIAAVRRLAGRALPAVVMSGHDEARVREDLGAPDIPILSKPVRPAELRSVLTAASLRLRPRPAPAA
>Mature_548_residues
MFGSRRRRDEAYDNEKAQALIRAVVIGLACLYLLPQIYGGIAPPEARFVAYLVTCHWAFAVALYGWITRNPGSNTPRRVV
TFVLDLGGMTYTMAVGGAPFLPLYAIVIRLIVGNGLRYGVTALKASTAAALVSIAITTYCNAYWRANPFLVLTLTAITVL
VPTYIYSLLERLRGAYEREQEASLSKSRFLAQASHDLRQPIHAISLFTACLRGARLGPEELQMVENIDRSLQSVSRLFKS
LLDISTLDSGRVEPRPEPVAIDDVIEDVRRQNSEAAQIAGTALRSRACAVVVRTDRALLTTMLQNIVNNAIKYAPGRPIL
IACRRRGDRLAVQVYDRGLGIPVAHQERVFDEFYQVRRRGDRDVEGVGLGLPIVRRLGELLDLAVTLRSVPGRGTCVTIG
NLPLARAPEPAALPAGLDPPATIGGLRVLLVEDDGAVLRATASLLETWGCRVQPETGIPDAVPEIDLLVTDFELGNGVTG
TDCIAAVRRLAGRALPAVVMSGHDEARVREDLGAPDIPILSKPVRPAELRSVLTAASLRLRPRPAPAA

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=341, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994928, Length=369, Percent_Identity=27.6422764227642, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789149, Length=283, Percent_Identity=29.3286219081272, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788713, Length=349, Percent_Identity=27.2206303724928, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081816, Length=394, Percent_Identity=28.1725888324873, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786912, Length=268, Percent_Identity=29.1044776119403, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=25.9090909090909, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=24.4239631336406, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082128, Length=258, Percent_Identity=24.8062015503876, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=24.2152466367713, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59505; Mature: 59505

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGSRRRRDEAYDNEKAQALIRAVVIGLACLYLLPQIYGGIAPPEARFVAYLVTCHWAFA
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VALYGWITRNPGSNTPRRVVTFVLDLGGMTYTMAVGGAPFLPLYAIVIRLIVGNGLRYGV
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHH
TALKASTAAALVSIAITTYCNAYWRANPFLVLTLTAITVLVPTYIYSLLERLRGAYEREQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EASLSKSRFLAQASHDLRQPIHAISLFTACLRGARLGPEELQMVENIDRSLQSVSRLFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLDISTLDSGRVEPRPEPVAIDDVIEDVRRQNSEAAQIAGTALRSRACAVVVRTDRALLT
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCHHHHH
TMLQNIVNNAIKYAPGRPILIACRRRGDRLAVQVYDRGLGIPVAHQERVFDEFYQVRRRG
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
DRDVEGVGLGLPIVRRLGELLDLAVTLRSVPGRGTCVTIGNLPLARAPEPAALPAGLDPP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
ATIGGLRVLLVEDDGAVLRATASLLETWGCRVQPETGIPDAVPEIDLLVTDFELGNGVTG
CCCCCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEECCCCCCH
TDCIAAVRRLAGRALPAVVMSGHDEARVREDLGAPDIPILSKPVRPAELRSVLTAASLRL
HHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
RPRPAPAA
CCCCCCCC
>Mature Secondary Structure
MFGSRRRRDEAYDNEKAQALIRAVVIGLACLYLLPQIYGGIAPPEARFVAYLVTCHWAFA
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VALYGWITRNPGSNTPRRVVTFVLDLGGMTYTMAVGGAPFLPLYAIVIRLIVGNGLRYGV
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHH
TALKASTAAALVSIAITTYCNAYWRANPFLVLTLTAITVLVPTYIYSLLERLRGAYEREQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EASLSKSRFLAQASHDLRQPIHAISLFTACLRGARLGPEELQMVENIDRSLQSVSRLFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLDISTLDSGRVEPRPEPVAIDDVIEDVRRQNSEAAQIAGTALRSRACAVVVRTDRALLT
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCHHHHH
TMLQNIVNNAIKYAPGRPILIACRRRGDRLAVQVYDRGLGIPVAHQERVFDEFYQVRRRG
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
DRDVEGVGLGLPIVRRLGELLDLAVTLRSVPGRGTCVTIGNLPLARAPEPAALPAGLDPP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
ATIGGLRVLLVEDDGAVLRATASLLETWGCRVQPETGIPDAVPEIDLLVTDFELGNGVTG
CCCCCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEECCCCCCH
TDCIAAVRRLAGRALPAVVMSGHDEARVREDLGAPDIPILSKPVRPAELRSVLTAASLRL
HHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
RPRPAPAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]