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The map label for this gene is fixK [H]

Identifier: 170747859

GI number: 170747859

Start: 1506456

End: 1507235

Strand: Reverse

Name: fixK [H]

Synonym: Mrad2831_1441

Alternate gene names: 170747859

Gene position: 1507235-1506456 (Counterclockwise)

Preceding gene: 170747860

Following gene: 170747856

Centisome position: 24.8

GC content: 71.79

Gene sequence:

>780_bases
TTGAACCCCTTCCTTCGCAAGCTGGCGCTGCGCGCGAACCTGGACGACGGCGACGTGGCCGCGCTGGGCGCGGCGACCGG
CGATGTCGCGCAGGTCGAGGCGGGCCGCGACCTGGTCTCGGAAGGCGTGCGGCCCGACGCCGCCTACGTGATCCTCGACG
GGTTCGCCTGCCGCTACAAGCTGGTGCCCGACGGGGGGCGCTCGATCCTCGCCTACCTCGTTCCCGGCGACGGCTGCGAT
CTGCACGCGTCGCTGCTCAACCGGGCGATCCACTCCGTGGCGACGCTGACGCCCTGCACCGTCGCCTCGGTCCCCTACCG
GACGATCAAGGAACTCGCCGCCCTCCGGCCCAACATCTACCGCGCCCTGTGGTGGTCGGTCCTGGTCGACGAGGCCGTCC
TGCAGGAATGGCTCGTCGGGGTGGGGCGCCGGTCCGCCGACAAGCAGGTCGCCCACATCATCTGCGAGCTCTTCGCGCGC
CTGAACGCCGTCGGCCTCGGCCTGGAGCCCGAGTACCGCCTTCCGCTGGCGCAGTCCGAACTGGCCGACACGGCGGGGCT
CTCGAACGTCCACCTCTCGCGCGTCCTCTCCGACCTGCGCAGGGCCGGGCTGATCGAGAGCGGGCGCAAGAGCGTGCGCG
TGCCGAATCTCGAACGTCTGCGGGAGTTTTCCGGGTTCACGCCCGACTACCTGCTCCTGCCCGGCCCGAACCCGGCCGTG
GAGGCGCTCGGCGCGCTGCCGCAGATGACCGGGCAGGTGCGGGTGGCGTCGAGCTCGTGA

Upstream 100 bases:

>100_bases
GGCGTGGCGATCATTTGATGACCTCGCGGAGTAAGGATAAGCCGTCGCGCGTTCACGCAGAGGCGCCTCCGCGCGCGGGG
CAGGACGTCCGGGAGATCCC

Downstream 100 bases:

>100_bases
GCCTCACCGGCCGTCGCTCTCGCAGCCCGTCTCGGGATCCTCGGCCACGCGGCGCCCGACCTGGGCCGTGAGCGGCACGC
GGATCGTGCAGCGCAGCCCG

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNPFLRKLALRANLDDGDVAALGAATGDVAQVEAGRDLVSEGVRPDAAYVILDGFACRYKLVPDGGRSILAYLVPGDGCD
LHASLLNRAIHSVATLTPCTVASVPYRTIKELAALRPNIYRALWWSVLVDEAVLQEWLVGVGRRSADKQVAHIICELFAR
LNAVGLGLEPEYRLPLAQSELADTAGLSNVHLSRVLSDLRRAGLIESGRKSVRVPNLERLREFSGFTPDYLLLPGPNPAV
EALGALPQMTGQVRVASSS

Sequences:

>Translated_259_residues
MNPFLRKLALRANLDDGDVAALGAATGDVAQVEAGRDLVSEGVRPDAAYVILDGFACRYKLVPDGGRSILAYLVPGDGCD
LHASLLNRAIHSVATLTPCTVASVPYRTIKELAALRPNIYRALWWSVLVDEAVLQEWLVGVGRRSADKQVAHIICELFAR
LNAVGLGLEPEYRLPLAQSELADTAGLSNVHLSRVLSDLRRAGLIESGRKSVRVPNLERLREFSGFTPDYLLLPGPNPAV
EALGALPQMTGQVRVASSS
>Mature_259_residues
MNPFLRKLALRANLDDGDVAALGAATGDVAQVEAGRDLVSEGVRPDAAYVILDGFACRYKLVPDGGRSILAYLVPGDGCD
LHASLLNRAIHSVATLTPCTVASVPYRTIKELAALRPNIYRALWWSVLVDEAVLQEWLVGVGRRSADKQVAHIICELFAR
LNAVGLGLEPEYRLPLAQSELADTAGLSNVHLSRVLSDLRRAGLIESGRKSVRVPNLERLREFSGFTPDYLLLPGPNPAV
EALGALPQMTGQVRVASSS

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27857; Mature: 27857

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFLRKLALRANLDDGDVAALGAATGDVAQVEAGRDLVSEGVRPDAAYVILDGFACRYK
CCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEE
LVPDGGRSILAYLVPGDGCDLHASLLNRAIHSVATLTPCTVASVPYRTIKELAALRPNIY
ECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCHHHH
RALWWSVLVDEAVLQEWLVGVGRRSADKQVAHIICELFARLNAVGLGLEPEYRLPLAQSE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LADTAGLSNVHLSRVLSDLRRAGLIESGRKSVRVPNLERLREFSGFTPDYLLLPGPNPAV
HHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCHHH
EALGALPQMTGQVRVASSS
HHHHHHHHCCCCEEEECCC
>Mature Secondary Structure
MNPFLRKLALRANLDDGDVAALGAATGDVAQVEAGRDLVSEGVRPDAAYVILDGFACRYK
CCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEE
LVPDGGRSILAYLVPGDGCDLHASLLNRAIHSVATLTPCTVASVPYRTIKELAALRPNIY
ECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCHHHH
RALWWSVLVDEAVLQEWLVGVGRRSADKQVAHIICELFARLNAVGLGLEPEYRLPLAQSE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LADTAGLSNVHLSRVLSDLRRAGLIESGRKSVRVPNLERLREFSGFTPDYLLLPGPNPAV
HHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCHHH
EALGALPQMTGQVRVASSS
HHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]