Click here to switch to the map view.

The map label for this gene is yogA [H]

Identifier: 170747843

GI number: 170747843

Start: 1489478

End: 1490497

Strand: Reverse

Name: yogA [H]

Synonym: Mrad2831_1425

Alternate gene names: 170747843

Gene position: 1490497-1489478 (Counterclockwise)

Preceding gene: 170747845

Following gene: 170747834

Centisome position: 24.52

GC content: 74.02

Gene sequence:

>1020_bases
ATGACCGGATCGACAGCCCGTGCCGTCATCGTCCAGCCCGGCGGCGGCTTCGACAAGGTGACCTTCGGCGAGCGGCCCGT
GCCGGAGCCCGGGCCCGGCGCGATCACCGTCCGCCTGCGGGCGAACTCCCTCAACTACCACGACTACGCGGTGGTCAGCG
GGATGTGGGGGCCGAGCGAGCCGCGCATCCCGATGTCCGACGGCGCCGGCGAGGTCGAGGCCGTGGGGCCGGGCATGACC
GAATTCGCCGTGGGCGACCGGGTGGTCAGCACGTTCTTCCCGACCTGGCTGGAGGGCACCCCGCTCGTCGAGGGCTTCGC
CACCGTGCCGGGCGACGGGGTCGACGGCTACGCCCGCGCGCGCGTCACCGCGCCCGCCACCGCCTTCACCCGCGCCCCGA
AGGGCTGGGACCACGCCGAGGCGGCGACGCTCACCACCGCCGCGCTGACCGCGTGGCGGGCACTGCACGCCGATGCCGGC
CTGAAGAGCGGCGACGTGGTGCTGGTGCAGGGGACCGGCGGCGTGTCGCTGTTCGGCCTGCAATTCGCCAAGGCGGCGGG
CGCCACGGTGATCGCCACCTCCTCCAGCGACGCCAAGCTGGAGCGCCTGCGCGGGCTCGGCGCCGACCACGTCATCAACT
ACCGCGCCGACCCGGCCTGGGGCGAGACCGCCCGGCGCCTGACCGACGGGCGCGGCGTCGACCACGTCCTCGACGTCGGC
GGGCCGGCGACGCTGGAGCAGTCCATGGCCGCCGTGCGGGTCGCCGGCCACATCTCGGTGATCGGTATCCTCTCGGGCGT
CGCGGGCGAGCTGCCGCTGGTGCCGGCGCTCCTGAAGCAGATCCGGCTGCAGGGCGTGCTGGTCGGCAGCCGGCGCCACC
AGATCGAGATGATCCGCGGCATCGACGCCTGCGGGCTGCGGCCGGTCATCGACCGCCGCTTCCCCCTCGACGACCTCGTC
GCGGCGTTCCGCCACCAGGAGAGCAACCGCCACTTCGGCAAGATCTGCGTCGAGATCTGA

Upstream 100 bases:

>100_bases
GCACCCGCGGCGGACTGCTCCGCTCCGTCGTCTCGACGCGGCGCGCCGGGCCTGCCAGTGTCCACCCCGGCGGCCGAGGC
CGCGGAGCGACAGGTGCGTC

Downstream 100 bases:

>100_bases
GCGTTCGGGCCGGGCCTGGGGCGTCGCGGCGGGCGGTGCGGCGGGCGTTCAGCGGAAGGCGATCCCGAGCGCTCCGGGCA
TCGCGACGACCCGGGGCGCC

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTGSTARAVIVQPGGGFDKVTFGERPVPEPGPGAITVRLRANSLNYHDYAVVSGMWGPSEPRIPMSDGAGEVEAVGPGMT
EFAVGDRVVSTFFPTWLEGTPLVEGFATVPGDGVDGYARARVTAPATAFTRAPKGWDHAEAATLTTAALTAWRALHADAG
LKSGDVVLVQGTGGVSLFGLQFAKAAGATVIATSSSDAKLERLRGLGADHVINYRADPAWGETARRLTDGRGVDHVLDVG
GPATLEQSMAAVRVAGHISVIGILSGVAGELPLVPALLKQIRLQGVLVGSRRHQIEMIRGIDACGLRPVIDRRFPLDDLV
AAFRHQESNRHFGKICVEI

Sequences:

>Translated_339_residues
MTGSTARAVIVQPGGGFDKVTFGERPVPEPGPGAITVRLRANSLNYHDYAVVSGMWGPSEPRIPMSDGAGEVEAVGPGMT
EFAVGDRVVSTFFPTWLEGTPLVEGFATVPGDGVDGYARARVTAPATAFTRAPKGWDHAEAATLTTAALTAWRALHADAG
LKSGDVVLVQGTGGVSLFGLQFAKAAGATVIATSSSDAKLERLRGLGADHVINYRADPAWGETARRLTDGRGVDHVLDVG
GPATLEQSMAAVRVAGHISVIGILSGVAGELPLVPALLKQIRLQGVLVGSRRHQIEMIRGIDACGLRPVIDRRFPLDDLV
AAFRHQESNRHFGKICVEI
>Mature_338_residues
TGSTARAVIVQPGGGFDKVTFGERPVPEPGPGAITVRLRANSLNYHDYAVVSGMWGPSEPRIPMSDGAGEVEAVGPGMTE
FAVGDRVVSTFFPTWLEGTPLVEGFATVPGDGVDGYARARVTAPATAFTRAPKGWDHAEAATLTTAALTAWRALHADAGL
KSGDVVLVQGTGGVSLFGLQFAKAAGATVIATSSSDAKLERLRGLGADHVINYRADPAWGETARRLTDGRGVDHVLDVGG
PATLEQSMAAVRVAGHISVIGILSGVAGELPLVPALLKQIRLQGVLVGSRRHQIEMIRGIDACGLRPVIDRRFPLDDLVA
AFRHQESNRHFGKICVEI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=344, Percent_Identity=27.3255813953488, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI22538444, Length=344, Percent_Identity=27.3255813953488, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI194239674, Length=310, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI13236495, Length=310, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI28557745, Length=343, Percent_Identity=29.1545189504373, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI47519420, Length=357, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI18379349, Length=360, Percent_Identity=24.4444444444444, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI194239676, Length=304, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI24308257, Length=369, Percent_Identity=22.7642276422764, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790485, Length=242, Percent_Identity=28.099173553719, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=349, Percent_Identity=26.0744985673352, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319520, Length=345, Percent_Identity=25.2173913043478, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=304, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=303, Percent_Identity=25.0825082508251, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=303, Percent_Identity=25.7425742574257, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=291, Percent_Identity=26.1168384879725, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920632, Length=224, Percent_Identity=27.6785714285714, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221330659, Length=222, Percent_Identity=27.9279279279279, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581345, Length=222, Percent_Identity=26.5765765765766, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35580; Mature: 35449

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSTARAVIVQPGGGFDKVTFGERPVPEPGPGAITVRLRANSLNYHDYAVVSGMWGPSE
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCC
PRIPMSDGAGEVEAVGPGMTEFAVGDRVVSTFFPTWLEGTPLVEGFATVPGDGVDGYARA
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
RVTAPATAFTRAPKGWDHAEAATLTTAALTAWRALHADAGLKSGDVVLVQGTGGVSLFGL
EEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEHH
QFAKAAGATVIATSSSDAKLERLRGLGADHVINYRADPAWGETARRLTDGRGVDHVLDVG
HHHHHCCCEEEEECCCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECC
GPATLEQSMAAVRVAGHISVIGILSGVAGELPLVPALLKQIRLQGVLVGSRRHQIEMIRG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHC
IDACGLRPVIDRRFPLDDLVAAFRHQESNRHFGKICVEI
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure 
TGSTARAVIVQPGGGFDKVTFGERPVPEPGPGAITVRLRANSLNYHDYAVVSGMWGPSE
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCC
PRIPMSDGAGEVEAVGPGMTEFAVGDRVVSTFFPTWLEGTPLVEGFATVPGDGVDGYARA
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
RVTAPATAFTRAPKGWDHAEAATLTTAALTAWRALHADAGLKSGDVVLVQGTGGVSLFGL
EEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEHH
QFAKAAGATVIATSSSDAKLERLRGLGADHVINYRADPAWGETARRLTDGRGVDHVLDVG
HHHHHCCCEEEEECCCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECC
GPATLEQSMAAVRVAGHISVIGILSGVAGELPLVPALLKQIRLQGVLVGSRRHQIEMIRG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHC
IDACGLRPVIDRRFPLDDLVAAFRHQESNRHFGKICVEI
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]