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The map label for this gene is fixK [H]

Identifier: 170747780

GI number: 170747780

Start: 1423195

End: 1423905

Strand: Reverse

Name: fixK [H]

Synonym: Mrad2831_1359

Alternate gene names: 170747780

Gene position: 1423905-1423195 (Counterclockwise)

Preceding gene: 170747781

Following gene: 170747779

Centisome position: 23.43

GC content: 73.0

Gene sequence:

>711_bases
GTGGAGAATCCGTTCGTACGGAAGATGGAGCGGCATGTCCGGCTCGCGCCGGAGGACCGCGCGGTGCTCGACGGGTTGGG
GCGCCTGCATGTCCGCCACGTCGCGGCCCGGCAGGATATCGAGGGGCAGCAGAGCGACCCGTACCACACGCACCTGATCC
TCGACGGCTGGGCCTGCCGGTACAAGCAGTTCCCGGACGGGCGGCGCGCGATCGTCGCGCTGCTCCTGCCGGGCGACCTG
TGCGACCCGTTCGTGTTCCGCCGGGCGCGGATGGACCACGCCATCGGCGCGCTGACCCCCGTCACCGTCGCGCGGATCGC
CCCGGAGCAGATCGGCGACGCGGTCCGCGGCCGGCCCGCGGTCGAGGAATGCCTGTGGCGGGAGTTGCTCGCCGCCACGG
CGATCCACCGCGAGTGGATCGCCAGCGTCGGCCGTCGCTCGGCGATCGAGCGGCTGGCGCACCTGTTCTGCGAGCTCCAC
CTCCGTCTGACGCGCGTCGGCCTCGCGGACGGGACGACGCTGCCGATGCCGGTGACCCAGCCCGACCTCGCGGACGCCCT
CGGCCAGACGAGCGTGCACATCAACCGCACCCTGAAGGAGCTGCGCACCGGCGGCCTCATCGCCCTGCGCAGCCGGCGCC
TGACGATCCGCGACCTCGACGGGCTGCGGGAGCGCGGGCTGTTCGATCCCGCCTACCTGCAGCTCTCCTGA

Upstream 100 bases:

>100_bases
CCGGTGCCTGCCCCGGCGCCTGCCCCGGCGCCTGCCCGGCCGGGCTCCGCCGTCCGGCCGCCGGCACGCCGGCGCCGCGG
TGATCTGACCGGAGGCGGAC

Downstream 100 bases:

>100_bases
GCGGCGCGGGCGATACGCCGCGGCATCGGAGGCCGCGCGCGCCTTGCCGCGGGGCGGCCGCGGCCGATCTGAGGCGGACC
TGTCATCCGCCACGGGCGCC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MENPFVRKMERHVRLAPEDRAVLDGLGRLHVRHVAARQDIEGQQSDPYHTHLILDGWACRYKQFPDGRRAIVALLLPGDL
CDPFVFRRARMDHAIGALTPVTVARIAPEQIGDAVRGRPAVEECLWRELLAATAIHREWIASVGRRSAIERLAHLFCELH
LRLTRVGLADGTTLPMPVTQPDLADALGQTSVHINRTLKELRTGGLIALRSRRLTIRDLDGLRERGLFDPAYLQLS

Sequences:

>Translated_236_residues
MENPFVRKMERHVRLAPEDRAVLDGLGRLHVRHVAARQDIEGQQSDPYHTHLILDGWACRYKQFPDGRRAIVALLLPGDL
CDPFVFRRARMDHAIGALTPVTVARIAPEQIGDAVRGRPAVEECLWRELLAATAIHREWIASVGRRSAIERLAHLFCELH
LRLTRVGLADGTTLPMPVTQPDLADALGQTSVHINRTLKELRTGGLIALRSRRLTIRDLDGLRERGLFDPAYLQLS
>Mature_236_residues
MENPFVRKMERHVRLAPEDRAVLDGLGRLHVRHVAARQDIEGQQSDPYHTHLILDGWACRYKQFPDGRRAIVALLLPGDL
CDPFVFRRARMDHAIGALTPVTVARIAPEQIGDAVRGRPAVEECLWRELLAATAIHREWIASVGRRSAIERLAHLFCELH
LRLTRVGLADGTTLPMPVTQPDLADALGQTSVHINRTLKELRTGGLIALRSRRLTIRDLDGLRERGLFDPAYLQLS

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26582; Mature: 26582

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENPFVRKMERHVRLAPEDRAVLDGLGRLHVRHVAARQDIEGQQSDPYHTHLILDGWACR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHH
YKQFPDGRRAIVALLLPGDLCDPFVFRRARMDHAIGALTPVTVARIAPEQIGDAVRGRPA
HCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHH
VEECLWRELLAATAIHREWIASVGRRSAIERLAHLFCELHLRLTRVGLADGTTLPMPVTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
PDLADALGQTSVHINRTLKELRTGGLIALRSRRLTIRDLDGLRERGLFDPAYLQLS
CHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure
MENPFVRKMERHVRLAPEDRAVLDGLGRLHVRHVAARQDIEGQQSDPYHTHLILDGWACR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHH
YKQFPDGRRAIVALLLPGDLCDPFVFRRARMDHAIGALTPVTVARIAPEQIGDAVRGRPA
HCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHH
VEECLWRELLAATAIHREWIASVGRRSAIERLAHLFCELHLRLTRVGLADGTTLPMPVTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
PDLADALGQTSVHINRTLKELRTGGLIALRSRRLTIRDLDGLRERGLFDPAYLQLS
CHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]