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The map label for this gene is bchE [H]

Identifier: 170747741

GI number: 170747741

Start: 1377170

End: 1378477

Strand: Reverse

Name: bchE [H]

Synonym: Mrad2831_1320

Alternate gene names: 170747741

Gene position: 1378477-1377170 (Counterclockwise)

Preceding gene: 170747742

Following gene: 170747740

Centisome position: 22.68

GC content: 73.24

Gene sequence:

>1308_bases
ATGAGAGTCGCGCTGGTCAACCCGCCCTGGGACTACCGCAACAGCATCTATTTCGGCTGCCGCGAGCCGCACCTGCCGCT
CGAACTCGGCTACTCCGCCGCGCTGCTGGCGGCCTCCGGCCACGAGACGCTGCTCGTGGACGCGCATCTCGAGGGCCTGA
CCGCCGAGGCCGCCGCCGACCGCGTCGCGGCCTTCGCCCCCGCCATGACCGTGGTGGCGACCGCCCCCACCTACCTGTTC
TGGCGCTGCGCCCCGCCGGAGCTGCGGGTGCCCCGCCTGTTCCTCGACACCCTGGCGGGCCGGGGCGGCACCACTGTCGC
GGTCGGCCCCCACGGCTCCGCGACGCCGCGTCCGGCGCTCGACAAGCTCGGCTGCGACATCGTCGTGCGCGGCGAGTGCG
AGGAGGTCGTCGCCCGGCTCGCCGGCCTGCCCGACCTGCGCCCGGAAACCCTGCGGGCCGTGCCCGGCCTCGCGGTCCGC
GACGGTGAGGCGGTCGCGGTGACCGGCCCGCCCCAGGCCGGCCGCTTCACCGACCTGCCGGCCCTGCGCTGGCCGGATCA
CTGGATCGCCCGGCACCGGCACCACCACCACCGGTTCGACGCCGCGCCGGACGGCCCTGGCGCCGAGGTCGAGGCCTCGC
GCGGCTGTCCCTATACCTGCTCGTTCTGCGCCAAGATCGACTTCCGCGACGCCTACCGGCGCCGGGATCTCGGGATCGTC
CTCGACGAGATCGACGGGCTGATCGCGCAGGGCGTCACCTACGTCTACTTCGTCGACGAGATCTTCCTGCCGCAGAAGCC
GCTTCTGGAGGCGCTGGTCACGCGGAACATCCGCTTCGGGATCCAGACCCGGATCGACCTGTGGAAGCCCGACCTGCTCG
ACCTCCTCGGGGCGGCGGGCTGCGTCTCCATCGAGGCCGGGATCGAGAGCCTGACGGAGGAAGGCCGCGCCGCCCTCGAC
AAGCGCTGCCGCGCCTCGACCGACGATCTCGCCGAGCGCCTGATCCACGCCCGCCGCACGGTGCCGTTCGTGCAGGCGAA
CCTCATCGCCGTGGCGGGCGACGATCCCGATCTCGTGGCGACGTGGCGCGACCGCCTGCGCGGCCAGGGGGTCTGGGCGA
ACGATCCCGTCCCGCTCTACCCGTATCCGAGCTCCCCCGATTACCGGCGCCTCTGGGGCGAGCCGGACGACCGGGCCTGG
GAGCGGGCGCACGCGCACTATCTCGGCCAGTTCACGCAGTTCAGCGAGATCCAGGACGAGCGTCCCCTGCCGCTGTCCGA
GCTGGAGGCCGCATGCGGGTGCCGCTGA

Upstream 100 bases:

>100_bases
CCTTCACGGGCTGGCGCGACGGCGTCGCCGACCTCGCCGGCTGGCTCGCCGCCGCGCGCGCGTCGTCGGATGGCGCGGCA
CCGATCGCGGGGGCGGCCGC

Downstream 100 bases:

>100_bases
GCCCCTCCCCGGCAGCGGCGCCGCACGTCCTGATGAGCGCGGACGCGGTGGGCGGCGTCTGGCAGTACAGCCTCGACCTC
GCGGCCGGCCTGGTCCGGCG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: Anaerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRVALVNPPWDYRNSIYFGCREPHLPLELGYSAALLAASGHETLLVDAHLEGLTAEAAADRVAAFAPAMTVVATAPTYLF
WRCAPPELRVPRLFLDTLAGRGGTTVAVGPHGSATPRPALDKLGCDIVVRGECEEVVARLAGLPDLRPETLRAVPGLAVR
DGEAVAVTGPPQAGRFTDLPALRWPDHWIARHRHHHHRFDAAPDGPGAEVEASRGCPYTCSFCAKIDFRDAYRRRDLGIV
LDEIDGLIAQGVTYVYFVDEIFLPQKPLLEALVTRNIRFGIQTRIDLWKPDLLDLLGAAGCVSIEAGIESLTEEGRAALD
KRCRASTDDLAERLIHARRTVPFVQANLIAVAGDDPDLVATWRDRLRGQGVWANDPVPLYPYPSSPDYRRLWGEPDDRAW
ERAHAHYLGQFTQFSEIQDERPLPLSELEAACGCR

Sequences:

>Translated_435_residues
MRVALVNPPWDYRNSIYFGCREPHLPLELGYSAALLAASGHETLLVDAHLEGLTAEAAADRVAAFAPAMTVVATAPTYLF
WRCAPPELRVPRLFLDTLAGRGGTTVAVGPHGSATPRPALDKLGCDIVVRGECEEVVARLAGLPDLRPETLRAVPGLAVR
DGEAVAVTGPPQAGRFTDLPALRWPDHWIARHRHHHHRFDAAPDGPGAEVEASRGCPYTCSFCAKIDFRDAYRRRDLGIV
LDEIDGLIAQGVTYVYFVDEIFLPQKPLLEALVTRNIRFGIQTRIDLWKPDLLDLLGAAGCVSIEAGIESLTEEGRAALD
KRCRASTDDLAERLIHARRTVPFVQANLIAVAGDDPDLVATWRDRLRGQGVWANDPVPLYPYPSSPDYRRLWGEPDDRAW
ERAHAHYLGQFTQFSEIQDERPLPLSELEAACGCR
>Mature_435_residues
MRVALVNPPWDYRNSIYFGCREPHLPLELGYSAALLAASGHETLLVDAHLEGLTAEAAADRVAAFAPAMTVVATAPTYLF
WRCAPPELRVPRLFLDTLAGRGGTTVAVGPHGSATPRPALDKLGCDIVVRGECEEVVARLAGLPDLRPETLRAVPGLAVR
DGEAVAVTGPPQAGRFTDLPALRWPDHWIARHRHHHHRFDAAPDGPGAEVEASRGCPYTCSFCAKIDFRDAYRRRDLGIV
LDEIDGLIAQGVTYVYFVDEIFLPQKPLLEALVTRNIRFGIQTRIDLWKPDLLDLLGAAGCVSIEAGIESLTEEGRAALD
KRCRASTDDLAERLIHARRTVPFVQANLIAVAGDDPDLVATWRDRLRGQGVWANDPVPLYPYPSSPDYRRLWGEPDDRAW
ERAHAHYLGQFTQFSEIQDERPLPLSELEAACGCR

Specific function: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis in anaerobic conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-prot

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011772
- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 47948; Mature: 47948

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALVNPPWDYRNSIYFGCREPHLPLELGYSAALLAASGHETLLVDAHLEGLTAEAAAD
CEEEEECCCCCCCCCEEEECCCCCCCCEECCCEEEEEECCCCEEEEECCCCCCCHHHHHH
RVAAFAPAMTVVATAPTYLFWRCAPPELRVPRLFLDTLAGRGGTTVAVGPHGSATPRPAL
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCH
DKLGCDIVVRGECEEVVARLAGLPDLRPETLRAVPGLAVRDGEAVAVTGPPQAGRFTDLP
HHCCCCEEEECCHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCC
ALRWPDHWIARHRHHHHRFDAAPDGPGAEVEASRGCPYTCSFCAKIDFRDAYRRRDLGIV
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCEEHHEEECCHHHHHHHCCCCEE
LDEIDGLIAQGVTYVYFVDEIFLPQKPLLEALVTRNIRFGIQTRIDLWKPDLLDLLGAAG
HHHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHCCCEECEEEEECCCCCHHHHHHCCCC
CVSIEAGIESLTEEGRAALDKRCRASTDDLAERLIHARRTVPFVQANLIAVAGDDPDLVA
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHH
TWRDRLRGQGVWANDPVPLYPYPSSPDYRRLWGEPDDRAWERAHAHYLGQFTQFSEIQDE
HHHHHHCCCCCCCCCCEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RPLPLSELEAACGCR
CCCCHHHHHHHHCCC
>Mature Secondary Structure
MRVALVNPPWDYRNSIYFGCREPHLPLELGYSAALLAASGHETLLVDAHLEGLTAEAAAD
CEEEEECCCCCCCCCEEEECCCCCCCCEECCCEEEEEECCCCEEEEECCCCCCCHHHHHH
RVAAFAPAMTVVATAPTYLFWRCAPPELRVPRLFLDTLAGRGGTTVAVGPHGSATPRPAL
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCH
DKLGCDIVVRGECEEVVARLAGLPDLRPETLRAVPGLAVRDGEAVAVTGPPQAGRFTDLP
HHCCCCEEEECCHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCC
ALRWPDHWIARHRHHHHRFDAAPDGPGAEVEASRGCPYTCSFCAKIDFRDAYRRRDLGIV
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCEEHHEEECCHHHHHHHCCCCEE
LDEIDGLIAQGVTYVYFVDEIFLPQKPLLEALVTRNIRFGIQTRIDLWKPDLLDLLGAAG
HHHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHCCCEECEEEEECCCCCHHHHHHCCCC
CVSIEAGIESLTEEGRAALDKRCRASTDDLAERLIHARRTVPFVQANLIAVAGDDPDLVA
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHH
TWRDRLRGQGVWANDPVPLYPYPSSPDYRRLWGEPDDRAWERAHAHYLGQFTQFSEIQDE
HHHHHHCCCCCCCCCCEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RPLPLSELEAACGCR
CCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10648776 [H]