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The map label for this gene is rfbB [C]

Identifier: 170747736

GI number: 170747736

Start: 1371492

End: 1372499

Strand: Reverse

Name: rfbB [C]

Synonym: Mrad2831_1315

Alternate gene names: 170747736

Gene position: 1372499-1371492 (Counterclockwise)

Preceding gene: 170747737

Following gene: 170747735

Centisome position: 22.58

GC content: 72.72

Gene sequence:

>1008_bases
ATGGTGGGAACCAGGAGCGGCATCCGAGTCCTCGTCGCCGGCGGCGCCGGGTTCATCGGCTCGCATCTGATCGACGCGCT
CCTCGCCGACGGGGCGCGCGTCACCTGTGTCGACAGCCTGCTGACCGGGCGGCGGGCGAATCTCGCCCATCTCGCCAACG
AGGCGCGGTTCGACTTCGTCGAGGCGGACGTGACGGAGCCGCTGCCGGCGCTGCCGCGGTTCGACTGGGTGTTCAACCTC
GCCTGCGCGGCCTCGCCGCCGCACTACCAGGCCGACCCGGTCCACACGATGATGACCAGCGTGCTGGGCACCGGCCGGCT
GCTGGAGGTCGCCCGGGACGCCGGGGCGCGCTTCCTCCAGGCCTCGACCAGCGAGGTCTACGGCGATCCGGAGCGGCACC
CGCAGCAGGAATCCTACTGGGGCAACGTCAACCCGACCGGGCCGCGGGCCTGCTACGACGAGGGCAAGCGCTCCGCCGAG
ACCCTGACCTTCGATTTCGAGCGCCAGCACGGCCTCGACATCCGCGTCGCGCGGATCTTCAACACCTACGGCCCGCGGAT
GCGCGCCGACGACGGCCGGGTCGTCTCCAACGTGATCTGCCAAGCCCTCGCGGGCGACGACATCACGGTCTACGGCAACG
GCGAGCAGACCCGCTCCTTCTGCTACGTCAGCGACTTGGTGGACGGGCTCCTCCGGCTGATGGCCGCGGAGACGCCCCTC
GCCGGCCCGGTCAACCTCGGCAACCCCCGCGAGCTGACGGTCGGCGCGCTGGTCGACCTCGTGGTGCGGATGACCGAGAC
CCCGTCCCGGATCGTGCGCCGGCCGCTGCCGGTGGACGATCCCCAGCGCCGCCGGCCGGACATCACCCGCGCCGAGACCC
TGCTCGGCTGGTCGCCCCGGGTGCCCCTGGAGGAGGGGCTGGAGGCGACGATCGCGTGGTTCTCCCGCGAGATCGCCCCG
CGCCGGGCCCGTCCGGCCATCGAGGAGCGCGCCGTTGTCGCCGGCTGA

Upstream 100 bases:

>100_bases
ACTCGAACGCTTCCTCGACGAAGCGGGCGCGCGGTCCGGATCCGCGCGCTCCGGCGCGCCCGATCTCGCGATCATCGGTC
ACTAGGCACTCGGGAGAATG

Downstream 100 bases:

>100_bases
GCCGCGCCCCGGGCGGCCGAGGCGCTGATGGCCGCCGCCCCCATCCGCGTCGGCCTCGTCGGGATCGGCAACTGCGCCTC
GTCCTTCGTGCAGGGACTGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MVGTRSGIRVLVAGGAGFIGSHLIDALLADGARVTCVDSLLTGRRANLAHLANEARFDFVEADVTEPLPALPRFDWVFNL
ACAASPPHYQADPVHTMMTSVLGTGRLLEVARDAGARFLQASTSEVYGDPERHPQQESYWGNVNPTGPRACYDEGKRSAE
TLTFDFERQHGLDIRVARIFNTYGPRMRADDGRVVSNVICQALAGDDITVYGNGEQTRSFCYVSDLVDGLLRLMAAETPL
AGPVNLGNPRELTVGALVDLVVRMTETPSRIVRRPLPVDDPQRRRPDITRAETLLGWSPRVPLEEGLEATIAWFSREIAP
RRARPAIEERAVVAG

Sequences:

>Translated_335_residues
MVGTRSGIRVLVAGGAGFIGSHLIDALLADGARVTCVDSLLTGRRANLAHLANEARFDFVEADVTEPLPALPRFDWVFNL
ACAASPPHYQADPVHTMMTSVLGTGRLLEVARDAGARFLQASTSEVYGDPERHPQQESYWGNVNPTGPRACYDEGKRSAE
TLTFDFERQHGLDIRVARIFNTYGPRMRADDGRVVSNVICQALAGDDITVYGNGEQTRSFCYVSDLVDGLLRLMAAETPL
AGPVNLGNPRELTVGALVDLVVRMTETPSRIVRRPLPVDDPQRRRPDITRAETLLGWSPRVPLEEGLEATIAWFSREIAP
RRARPAIEERAVVAG
>Mature_335_residues
MVGTRSGIRVLVAGGAGFIGSHLIDALLADGARVTCVDSLLTGRRANLAHLANEARFDFVEADVTEPLPALPRFDWVFNL
ACAASPPHYQADPVHTMMTSVLGTGRLLEVARDAGARFLQASTSEVYGDPERHPQQESYWGNVNPTGPRACYDEGKRSAE
TLTFDFERQHGLDIRVARIFNTYGPRMRADDGRVVSNVICQALAGDDITVYGNGEQTRSFCYVSDLVDGLLRLMAAETPL
AGPVNLGNPRELTVGALVDLVVRMTETPSRIVRRPLPVDDPQRRRPDITRAETLLGWSPRVPLEEGLEATIAWFSREIAP
RRARPAIEERAVVAG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=310, Percent_Identity=52.9032258064516, Blast_Score=351, Evalue=4e-97,
Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=27.4143302180685, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=28.6549707602339, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=27.7611940298507, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788589, Length=347, Percent_Identity=25.3602305475504, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786974, Length=338, Percent_Identity=28.698224852071, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788366, Length=360, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=331, Percent_Identity=25.0755287009063, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=311, Percent_Identity=51.1254019292604, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=27.2455089820359, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=27.8592375366569, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356223, Length=311, Percent_Identity=53.6977491961415, Blast_Score=357, Evalue=5e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 36654; Mature: 36654

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGTRSGIRVLVAGGAGFIGSHLIDALLADGARVTCVDSLLTGRRANLAHLANEARFDFV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHEE
EADVTEPLPALPRFDWVFNLACAASPPHYQADPVHTMMTSVLGTGRLLEVARDAGARFLQ
ECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ASTSEVYGDPERHPQQESYWGNVNPTGPRACYDEGKRSAETLTFDFERQHGLDIRVARIF
CCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCEEHHHHH
NTYGPRMRADDGRVVSNVICQALAGDDITVYGNGEQTRSFCYVSDLVDGLLRLMAAETPL
HHCCCCEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
AGPVNLGNPRELTVGALVDLVVRMTETPSRIVRRPLPVDDPQRRRPDITRAETLLGWSPR
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCCHHHHHHHCCCCC
VPLEEGLEATIAWFSREIAPRRARPAIEERAVVAG
CCHHHHHHHHHHHHHHHHCCHHCCCCHHHCCCCCC
>Mature Secondary Structure
MVGTRSGIRVLVAGGAGFIGSHLIDALLADGARVTCVDSLLTGRRANLAHLANEARFDFV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHEE
EADVTEPLPALPRFDWVFNLACAASPPHYQADPVHTMMTSVLGTGRLLEVARDAGARFLQ
ECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ASTSEVYGDPERHPQQESYWGNVNPTGPRACYDEGKRSAETLTFDFERQHGLDIRVARIF
CCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCEEHHHHH
NTYGPRMRADDGRVVSNVICQALAGDDITVYGNGEQTRSFCYVSDLVDGLLRLMAAETPL
HHCCCCEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
AGPVNLGNPRELTVGALVDLVVRMTETPSRIVRRPLPVDDPQRRRPDITRAETLLGWSPR
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCCHHHHHHHCCCCC
VPLEEGLEATIAWFSREIAPRRARPAIEERAVVAG
CCHHHHHHHHHHHHHHHHCCHHCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]