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The map label for this gene is fcl [C]

Identifier: 170747647

GI number: 170747647

Start: 1284840

End: 1285817

Strand: Reverse

Name: fcl [C]

Synonym: Mrad2831_1220

Alternate gene names: 170747647

Gene position: 1285817-1284840 (Counterclockwise)

Preceding gene: 170747648

Following gene: 170747646

Centisome position: 21.16

GC content: 71.06

Gene sequence:

>978_bases
GTGGCTGCCGTCTCCCGATCCTCCTTGCCCGAGACCGTCGTCGTCGTCGGTGCGACCGGGTTCGTCGGCCGCAACCTCGT
CGCGCATCTCGCCGACCGCGTGCCGCGGCTGATCGCGGTCAGCCCCTCCGGCCGCGCGGTGCCGGGGGCGCATGAGGCCG
CGCGCTTCGATGCCCTGGAGGCGCTGTCGGTCCCCGCCGACGCGATCGTCGTCAACGTCGCCGCGCAGCGCTACGACGCC
GACAAGTTCGAGACGGCGCAGAGCGACCTGCTGACCGGCAACGCCGAGATCGCCAACCGCCTCTACCGGTTCTGCCTGAC
GAAGGGCATCCGTGAGGTGCGCGCGGCCTCGAGCGTCGCGGTCTACCCGCCGGCCCTCGATCCCCTCGACGACGCGCAGG
CTGTCGATCTCAACGCGCCGCCCAACCGGAACGAGGCCTTCTACGCGTGGTCGAAGCGCTGGTCGGAGGTGATCGCCGAC
CTCCACGCCGAGCGCTACGGGATCCACACGATCAGCTTCCGCCTGAGCAACCCCTACGGCCCCCACGACTCGACGGATCC
GCGCAAGGCGCACGTCCTGCCGGCCTTCGTGATGCGCGCGCTCGCACCGGGGGAGCGCTTCGAGATCAAGGGCAACCCGC
TGGTCGAGCGCGACTTCATCTACGTCGGCGACGTCGTCGACGTGTTCGCCCGCTCCCTCGAGATGCGGGAGACGAGCGAC
GCGCTCAACCTCTGCCGCGGCGAGACCACGACCCTGCTCGACCTTGCCCGTACCATCCTGGAGGTGGCGGGCGAGAATCG
GCCGGTGCACGCCCCCGACACGGCGACCCACGGTGTGCTCGCCCGCCGCTCGACCAACCAGCGGCTGCGTGAGCGATTCG
GGATGTCGAGCTTCACGGGACTCGCGGACGGGCTGCGGCCGACGATCGCGTGGTACCGCGAGGCCTATCGCGACGGGGCT
CAGCATGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCGAGCGCCGCTACCTCGAGAACGGCGGCGCCTTCATCGTTCCGGTGCCGGAACTCAAGGTGATCACCGCCGCCGAC
CTGCCGAAGGCGGTCTGAGC

Downstream 100 bases:

>100_bases
GCGCGACATCGTCATCGGGGCGGACGGCTTCCTCGGGCGCAACCTCGTCCCGCGGCTCCGGGCCGAGGGCCGCGAGGTCG
TCGAGATCGGTCGGGCGGCG

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: GDP-Fucose Synthetase; UDP-Glucose 4-Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; Nucleoside-Diphosphate-Sugar Epimerase; GDP-L-Fucose Synthase; NAD Dependent Epimerase/Dehydratase Family; GDP-L-Fucose Synthetase; DTDP-Glucose 4 6-Dehydratase; Sugar Epimerase BlmG; Epimerase; GDP-Fucose Synthetase NAD Dependent Epimerase/Dehydratase; NDP-Sugar Oxidoreductase; Nucleoside-Diphosphate-Sugar Epimerases; GDP-D-Mannose Dehydratase; Ata17 Protein; Nucleotide Sugar Epimerase/Dehydratase; DTDP-Glucose 4-6-Dehydratase; NDP-Hexose 4 6-Dehydratase; UDP-Glucose 4-Epimerase-Like Protein; GDP-L-Fucose Synthase Protein; NAD Dependent Epimerase; UDP-Glucose 4-Epimerase Related Protein; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleotide-Sugar Dehydratase; GDP-Fucose Synthetase Nad Dependent Epimerase/Dehydratase; DTDP-Glucose Dehydratase; Hexose-4-Ketoreductase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAAVSRSSLPETVVVVGATGFVGRNLVAHLADRVPRLIAVSPSGRAVPGAHEAARFDALEALSVPADAIVVNVAAQRYDA
DKFETAQSDLLTGNAEIANRLYRFCLTKGIREVRAASSVAVYPPALDPLDDAQAVDLNAPPNRNEAFYAWSKRWSEVIAD
LHAERYGIHTISFRLSNPYGPHDSTDPRKAHVLPAFVMRALAPGERFEIKGNPLVERDFIYVGDVVDVFARSLEMRETSD
ALNLCRGETTTLLDLARTILEVAGENRPVHAPDTATHGVLARRSTNQRLRERFGMSSFTGLADGLRPTIAWYREAYRDGA
QHAGA

Sequences:

>Translated_325_residues
MAAVSRSSLPETVVVVGATGFVGRNLVAHLADRVPRLIAVSPSGRAVPGAHEAARFDALEALSVPADAIVVNVAAQRYDA
DKFETAQSDLLTGNAEIANRLYRFCLTKGIREVRAASSVAVYPPALDPLDDAQAVDLNAPPNRNEAFYAWSKRWSEVIAD
LHAERYGIHTISFRLSNPYGPHDSTDPRKAHVLPAFVMRALAPGERFEIKGNPLVERDFIYVGDVVDVFARSLEMRETSD
ALNLCRGETTTLLDLARTILEVAGENRPVHAPDTATHGVLARRSTNQRLRERFGMSSFTGLADGLRPTIAWYREAYRDGA
QHAGA
>Mature_324_residues
AAVSRSSLPETVVVVGATGFVGRNLVAHLADRVPRLIAVSPSGRAVPGAHEAARFDALEALSVPADAIVVNVAAQRYDAD
KFETAQSDLLTGNAEIANRLYRFCLTKGIREVRAASSVAVYPPALDPLDDAQAVDLNAPPNRNEAFYAWSKRWSEVIADL
HAERYGIHTISFRLSNPYGPHDSTDPRKAHVLPAFVMRALAPGERFEIKGNPLVERDFIYVGDVVDVFARSLEMRETSDA
LNLCRGETTTLLDLARTILEVAGENRPVHAPDTATHGVLARRSTNQRLRERFGMSSFTGLADGLRPTIAWYREAYRDGAQ
HAGA

Specific function: Two Step Nadp-Dependent Conversion Of Gdp-4-Dehydro-6- Deoxy-D-Mannose To Gdp-Fucose, Involving An Epimerase And A Reductase Reaction. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4507709, Length=235, Percent_Identity=22.9787234042553, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17554428, Length=229, Percent_Identity=21.8340611353712, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922778, Length=319, Percent_Identity=22.884012539185, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.271

Molecular weight: Translated: 35468; Mature: 35337

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVSRSSLPETVVVVGATGFVGRNLVAHLADRVPRLIAVSPSGRAVPGAHEAARFDALE
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHH
ALSVPADAIVVNVAAQRYDADKFETAQSDLLTGNAEIANRLYRFCLTKGIREVRAASSVA
HHCCCHHHHEEEHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEE
VYPPALDPLDDAQAVDLNAPPNRNEAFYAWSKRWSEVIADLHAERYGIHTISFRLSNPYG
ECCCCCCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCC
PHDSTDPRKAHVLPAFVMRALAPGERFEIKGNPLVERDFIYVGDVVDVFARSLEMRETSD
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEECCEEECHHHHHHHHHHHHHHHHH
ALNLCRGETTTLLDLARTILEVAGENRPVHAPDTATHGVLARRSTNQRLRERFGMSSFTG
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
LADGLRPTIAWYREAYRDGAQHAGA
HHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAVSRSSLPETVVVVGATGFVGRNLVAHLADRVPRLIAVSPSGRAVPGAHEAARFDALE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHH
ALSVPADAIVVNVAAQRYDADKFETAQSDLLTGNAEIANRLYRFCLTKGIREVRAASSVA
HHCCCHHHHEEEHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEE
VYPPALDPLDDAQAVDLNAPPNRNEAFYAWSKRWSEVIADLHAERYGIHTISFRLSNPYG
ECCCCCCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCC
PHDSTDPRKAHVLPAFVMRALAPGERFEIKGNPLVERDFIYVGDVVDVFARSLEMRETSD
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEECCEEECHHHHHHHHHHHHHHHHH
ALNLCRGETTTLLDLARTILEVAGENRPVHAPDTATHGVLARRSTNQRLRERFGMSSFTG
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
LADGLRPTIAWYREAYRDGAQHAGA
HHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA