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The map label for this gene is fcl [C]

Identifier: 170747646

GI number: 170747646

Start: 1283930

End: 1284853

Strand: Reverse

Name: fcl [C]

Synonym: Mrad2831_1219

Alternate gene names: 170747646

Gene position: 1284853-1283930 (Counterclockwise)

Preceding gene: 170747647

Following gene: 170747645

Centisome position: 21.14

GC content: 63.74

Gene sequence:

>924_bases
ATGCCGGGGCCTGAGCGCGACATCGTCATCGGGGCGGACGGCTTCCTCGGGCGCAACCTCGTCCCGCGGCTCCGGGCCGA
GGGCCGCGAGGTCGTCGAGATCGGTCGGGCGGCGGGTGACCTCTCCGAGTGGGCGAATGTCGAGCGGGCGCTGCGCGCGG
CGCCGCCGGCCGGCAGGATCTTCCACGTGGTGACCCGCCAGCGGACCGGCGCGGTGCAGTACGACATCCAGGGCGAACTC
CTGGCAATCAACGCCCGGGTCCACCTGAACGTCCTCGAGGCGTGGCGCCTGTTCCAGCCGCAGGCCAAGCTCGTCTCCAC
GGGCTCGTCCTGCACCTATCCGGAATCGCCGGAGCCGCTCCCCGAGGCGCGGTTCGGGCAGGGACCCACGCACCCCTCGG
TGATCGGCTACGGGCTCGCCAAGCAGGTCCTGGCGACGGGCTCGGCGGCCTACGCGCACCAGTACGGCCTGAGCTTCCTG
CACTGCGTGCTGGCGACCCTGTACGGGCCGCACGACAACAAGGCCTCCGACCGCAGCCACTTCGTCGGCGCCCTGCTCGA
CAGGGCCGTGCGGGAGAAGGCGGCGGGCGCATTACAATTTGAAGTATGGGGCAACCCGCGGACTGTCCGCGAAATACTCC
ACGTTGAAGACCAGATAAAAGCGATCTTGGCGGCAGAACGGCAGTTCAATAATGAAATACTAAACTGCGCCGCGAACAAA
CCCATCACTGTTGGAGAGGTTGCACAGACGGCGCTGGCTGCTTTGAAGTGGAAAGTCCCAATAAAGACTTCGCTTAATAG
CTTCCAGGGGGCGGCCATTAAGGTAATAGACTCATCCCGCTTTTTGAATAAAACGGGATGGCGACCGAAAATCGATCTGA
AATCTGGCCTTCGACAGCTGTTAGCGAGAGAGTATCAAATATGA

Upstream 100 bases:

>100_bases
CTGCGTGAGCGATTCGGGATGTCGAGCTTCACGGGACTCGCGGACGGGCTGCGGCCGACGATCGCGTGGTACCGCGAGGC
CTATCGCGACGGGGCTCAGC

Downstream 100 bases:

>100_bases
CGTATTATGACCGTTGGGAAAACCCCTACAGCAGTAGCTAATCGACTTCCAAGAACTTAAATGGTGAGCTTGATCGTCAC
CGAAGACTGGTACTATTATA

Product: GDP-L-fucose synthase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPGPERDIVIGADGFLGRNLVPRLRAEGREVVEIGRAAGDLSEWANVERALRAAPPAGRIFHVVTRQRTGAVQYDIQGEL
LAINARVHLNVLEAWRLFQPQAKLVSTGSSCTYPESPEPLPEARFGQGPTHPSVIGYGLAKQVLATGSAAYAHQYGLSFL
HCVLATLYGPHDNKASDRSHFVGALLDRAVREKAAGALQFEVWGNPRTVREILHVEDQIKAILAAERQFNNEILNCAANK
PITVGEVAQTALAALKWKVPIKTSLNSFQGAAIKVIDSSRFLNKTGWRPKIDLKSGLRQLLAREYQI

Sequences:

>Translated_307_residues
MPGPERDIVIGADGFLGRNLVPRLRAEGREVVEIGRAAGDLSEWANVERALRAAPPAGRIFHVVTRQRTGAVQYDIQGEL
LAINARVHLNVLEAWRLFQPQAKLVSTGSSCTYPESPEPLPEARFGQGPTHPSVIGYGLAKQVLATGSAAYAHQYGLSFL
HCVLATLYGPHDNKASDRSHFVGALLDRAVREKAAGALQFEVWGNPRTVREILHVEDQIKAILAAERQFNNEILNCAANK
PITVGEVAQTALAALKWKVPIKTSLNSFQGAAIKVIDSSRFLNKTGWRPKIDLKSGLRQLLAREYQI
>Mature_306_residues
PGPERDIVIGADGFLGRNLVPRLRAEGREVVEIGRAAGDLSEWANVERALRAAPPAGRIFHVVTRQRTGAVQYDIQGELL
AINARVHLNVLEAWRLFQPQAKLVSTGSSCTYPESPEPLPEARFGQGPTHPSVIGYGLAKQVLATGSAAYAHQYGLSFLH
CVLATLYGPHDNKASDRSHFVGALLDRAVREKAAGALQFEVWGNPRTVREILHVEDQIKAILAAERQFNNEILNCAANKP
ITVGEVAQTALAALKWKVPIKTSLNSFQGAAIKVIDSSRFLNKTGWRPKIDLKSGLRQLLAREYQI

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=290, Percent_Identity=25.1724137931034, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788365, Length=206, Percent_Identity=32.5242718446602, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17554428, Length=285, Percent_Identity=20.7017543859649, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922778, Length=266, Percent_Identity=25.5639097744361, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 33605; Mature: 33474

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGPERDIVIGADGFLGRNLVPRLRAEGREVVEIGRAAGDLSEWANVERALRAAPPAGRI
CCCCCCCEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCE
FHVVTRQRTGAVQYDIQGELLAINARVHLNVLEAWRLFQPQAKLVSTGSSCTYPESPEPL
EEEEECCCCCCEEEECCCCEEEEEEEEEEEHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
PEARFGQGPTHPSVIGYGLAKQVLATGSAAYAHQYGLSFLHCVLATLYGPHDNKASDRSH
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
FVGALLDRAVREKAAGALQFEVWGNPRTVREILHVEDQIKAILAAERQFNNEILNCAANK
HHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PITVGEVAQTALAALKWKVPIKTSLNSFQGAAIKVIDSSRFLNKTGWRPKIDLKSGLRQL
CCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHH
LAREYQI
HHHHCCC
>Mature Secondary Structure 
PGPERDIVIGADGFLGRNLVPRLRAEGREVVEIGRAAGDLSEWANVERALRAAPPAGRI
CCCCCCEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCE
FHVVTRQRTGAVQYDIQGELLAINARVHLNVLEAWRLFQPQAKLVSTGSSCTYPESPEPL
EEEEECCCCCCEEEECCCCEEEEEEEEEEEHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
PEARFGQGPTHPSVIGYGLAKQVLATGSAAYAHQYGLSFLHCVLATLYGPHDNKASDRSH
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
FVGALLDRAVREKAAGALQFEVWGNPRTVREILHVEDQIKAILAAERQFNNEILNCAANK
HHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PITVGEVAQTALAALKWKVPIKTSLNSFQGAAIKVIDSSRFLNKTGWRPKIDLKSGLRQL
CCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHH
LAREYQI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]