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The map label for this gene is hyi [C]

Identifier: 170747565

GI number: 170747565

Start: 1193502

End: 1194284

Strand: Reverse

Name: hyi [C]

Synonym: Mrad2831_1133

Alternate gene names: 170747565

Gene position: 1194284-1193502 (Counterclockwise)

Preceding gene: 170747566

Following gene: 170747564

Centisome position: 19.65

GC content: 71.14

Gene sequence:

>783_bases
ATGCCGCGCTTTGCCGCAAACCTGAGCCTCATGTTCAACGAGGTGCCCTTCCTCGACCGGTTCGAGGCCGCGGCCCGCGC
CGGGTTCGACGCCGTCGAGTTCCTGTTCCCCTACGAGCACCCGCCGGAGGCCATCGGCGAGCGGCTCCGGGCCAACGGCC
TGACCCAGGCCCTGTTCAACCTGCCGCCGGGGGACTTCGCCAAGGGCGAGCGCGGGATCGCGGCTTTCCCCGAGCGCTTC
GACGAGCTGAAGGCCGGTGTCGAGACGGCACTCCGCTACGCCGAGGCGACCGGCGTCGGGCGGCTCCACCTGATGGCGGG
CCTCGCGCCCTGGGACGACGCCGCGGCGGCCGAGTCCTACCGCCGGGCCGTCACCTGGACGGCCGAGCGGCTGGCGTCGG
CCGGCCTCGACCTCGTCCTGGAGCCGATCAACGGCCGGAACATGCCGGGCTACTTCCTCAACGATTTCGGCCGGGCCGCG
GACCTGATCCGCGACCTCGCCCTGCCGAACCTGAAGCTGCAGTTCGACCTCTACCACTGCCAGATCCTGCACGGCGACGT
GACCATGCGGCTGCGCGCCCTGATGCCGCTAATCGGCCACGTCCAGACCGCGAGCGTGCCCGAGCGGCACGAGCCCGGCA
CTGGCGAAATGGCCGACTTCTTCCTGTTCGACGAGCTGGACCGGCTCGGCTACCGCGGCTTCGTCGGCTGCGAGTACATT
CCGGCCGCCGACACCGTCGCGGGCCTCGGCTGGCTCGCCGCCTACAAGGAGAGCGCCCGATGA

Upstream 100 bases:

>100_bases
GGGACGACGACGCCTCGGTGGCGCGGCTCTACGCGCAGGTCTCGGGCGCGAAGCTGCCGGGCCAGAAGTAGCCGGATCGA
GCGCAGGGAGTTCGAAGACA

Downstream 100 bases:

>100_bases
GCCTCGCGCTGGGATGCGTCGCCGACGACTACACGGGCGCCTCGGACCTCGCCAACACGCTGACCAAGGCGGGCCTGCGC
ACGATCCAGACCATCGGCGT

Product: hydroxypyruvate isomerase

Products: 2-hydroxy-3-oxopropanoate

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPRFAANLSLMFNEVPFLDRFEAAARAGFDAVEFLFPYEHPPEAIGERLRANGLTQALFNLPPGDFAKGERGIAAFPERF
DELKAGVETALRYAEATGVGRLHLMAGLAPWDDAAAAESYRRAVTWTAERLASAGLDLVLEPINGRNMPGYFLNDFGRAA
DLIRDLALPNLKLQFDLYHCQILHGDVTMRLRALMPLIGHVQTASVPERHEPGTGEMADFFLFDELDRLGYRGFVGCEYI
PAADTVAGLGWLAAYKESAR

Sequences:

>Translated_260_residues
MPRFAANLSLMFNEVPFLDRFEAAARAGFDAVEFLFPYEHPPEAIGERLRANGLTQALFNLPPGDFAKGERGIAAFPERF
DELKAGVETALRYAEATGVGRLHLMAGLAPWDDAAAAESYRRAVTWTAERLASAGLDLVLEPINGRNMPGYFLNDFGRAA
DLIRDLALPNLKLQFDLYHCQILHGDVTMRLRALMPLIGHVQTASVPERHEPGTGEMADFFLFDELDRLGYRGFVGCEYI
PAADTVAGLGWLAAYKESAR
>Mature_259_residues
PRFAANLSLMFNEVPFLDRFEAAARAGFDAVEFLFPYEHPPEAIGERLRANGLTQALFNLPPGDFAKGERGIAAFPERFD
ELKAGVETALRYAEATGVGRLHLMAGLAPWDDAAAAESYRRAVTWTAERLASAGLDLVLEPINGRNMPGYFLNDFGRAAD
LIRDLALPNLKLQFDLYHCQILHGDVTMRLRALMPLIGHVQTASVPERHEPGTGEMADFFLFDELDRLGYRGFVGCEYIP
AADTVAGLGWLAAYKESAR

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=262, Percent_Identity=46.1832061068702, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI300068964, Length=243, Percent_Identity=44.8559670781893, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1786718, Length=258, Percent_Identity=41.4728682170543, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1789095, Length=260, Percent_Identity=42.3076923076923, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17552022, Length=253, Percent_Identity=36.7588932806324, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17530883, Length=259, Percent_Identity=34.7490347490347, Blast_Score=157, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22

Molecular weight: Translated: 28721; Mature: 28590

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFAANLSLMFNEVPFLDRFEAAARAGFDAVEFLFPYEHPPEAIGERLRANGLTQALFN
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
LPPGDFAKGERGIAAFPERFDELKAGVETALRYAEATGVGRLHLMAGLAPWDDAAAAESY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
RRAVTWTAERLASAGLDLVLEPINGRNMPGYFLNDFGRAADLIRDLALPNLKLQFDLYHC
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
QILHGDVTMRLRALMPLIGHVQTASVPERHEPGTGEMADFFLFDELDRLGYRGFVGCEYI
EEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAADTVAGLGWLAAYKESAR
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRFAANLSLMFNEVPFLDRFEAAARAGFDAVEFLFPYEHPPEAIGERLRANGLTQALFN
CCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
LPPGDFAKGERGIAAFPERFDELKAGVETALRYAEATGVGRLHLMAGLAPWDDAAAAESY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
RRAVTWTAERLASAGLDLVLEPINGRNMPGYFLNDFGRAADLIRDLALPNLKLQFDLYHC
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
QILHGDVTMRLRALMPLIGHVQTASVPERHEPGTGEMADFFLFDELDRLGYRGFVGCEYI
EEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAADTVAGLGWLAAYKESAR
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: hydroxypyruvate

Specific reaction: hydroxypyruvate = 2-hydroxy-3-oxopropanoate; Other Name(s): hydroxypyruvate ketol-isomerase

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]