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The map label for this gene is rlmN [H]

Identifier: 170747554

GI number: 170747554

Start: 1183013

End: 1184305

Strand: Reverse

Name: rlmN [H]

Synonym: Mrad2831_1122

Alternate gene names: 170747554

Gene position: 1184305-1183013 (Counterclockwise)

Preceding gene: 170747556

Following gene: 170747549

Centisome position: 19.49

GC content: 70.22

Gene sequence:

>1293_bases
ATGGATCGTTTGAACGCCATGGCGACGCCGTCGCTCGACAGCGCCCGCGCGGCCGATGCCGCGATCGAGAAGGCGCCGGA
TCTCCTCCCCGAGGCGCTGCCCGGCCGCCGCCCGTCGCTGGTCGGCCTGACCCGCGACGCCCTCAAGGCGCAGCTCATCG
GCATGGGCGTGCCCGAGCGCGAGAGCCGGATGCGCGCCGGCCAGGTTTGGCACTGGGTCAATTTCCGCGGGGCGTCCGAC
TTCGCCGAGATGACCAATGTCGGCAAGGCGCTGAAGGCGCAGCTCGCCGAGCACTTCACCCTGGAGCGGCCCGAGGTGGC
GAGCCGGCAGGTCTCCCGCGACGGCACCCGCAAGTGGCTGCTGCGCATGGCGCCTACCAACCGGCAGGAGCACAATCGCG
GCGCCGAGATCGAGTGCGTATACATTCCCGGCCCGGACCGGGGCACGCTCTGCGTGTCCTCCCAGGTCGGCTGCACCCTG
ACCTGCTCGTTCTGCCACACCGGCACGCAGCGCCTCGTGCGCAACCTGTCGGCCGCCGAGATCGTCCAGCAGCTCGTCAC
CGCCCGGGACGAGCTCGGCGACTGGCCCGGCCAGATGCCGAGCCGCGACGCGGGCGGCTCCGGCGAGGTCGGCCGGCTCG
TGACCAACATCGTGTTCATGGGCATGGGCGAGCCCCTCTACAACCTCGACGCCGTGGTCGACGCGGTCGGGGTGATGTCG
GACCAGGAGGGCCTCGGCCTGTCCCGCCGCCGGATCACGGTCTCGACCTCGGGGGTCGTGCCGCAGATCCCCCGGCTCGG
CGAGCAGGCCAACGCCATGCTGGCGATCTCGCTGCACGCCGTGCGCGACGACCTGCGCGACGAGCTGGTGCCGCTCAACC
GGAAATACCCAATCGCCGAACTGCTCGCGGCCTGCCGGGCCTATCCGGGCCTGAGCAACGCCCGCCGGATCACCTTCGAG
TACGTGATGCTGAAGGGCGTCAACGATTCGGACGCGGATGCACGGGAATTGGTCCGCCTGCTCAAGGGCATCCCGGCCAA
GATCAACCTGATCCCGTTCAACCCCTGGCCGGGCAGTCGCTACGAATGCTCCGACTGGGACCGGATCGAGCGCTTCTCCG
AGATCGTGTTCAACGCCGGCTACGCCTCGCCGGTGCGCACGCCCCGCGGCAGGGACATCCTGGCGGCCTGCGGCCAGCTC
AAGAGCGAGACCGAGAAGCTGCGCGCCCGCGCCCGGTTGATGCAGGAGGAGGGCATCGGCGTGGAGAGTTTTTACGCGGG
CGCCGACGACTGA

Upstream 100 bases:

>100_bases
GCTCGGGTGTCGGTCGCCATGGCTCTCCGTCAGGGCCCCTGTGGGCGGCGCGCATTCGCATTCGCCGCCGCGATCGCCTA
TATCAGACCCTTTCCAACGT

Downstream 100 bases:

>100_bases
GCGACGGTCGAACGGAAGACATCCAACCCGCATCCTCATCCTGAGGTGCGGCGAAGCCGCCTCGAAGGAGCCCGCCAGAA
CCCGCTGTGATTCCTGGAAC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDRLNAMATPSLDSARAADAAIEKAPDLLPEALPGRRPSLVGLTRDALKAQLIGMGVPERESRMRAGQVWHWVNFRGASD
FAEMTNVGKALKAQLAEHFTLERPEVASRQVSRDGTRKWLLRMAPTNRQEHNRGAEIECVYIPGPDRGTLCVSSQVGCTL
TCSFCHTGTQRLVRNLSAAEIVQQLVTARDELGDWPGQMPSRDAGGSGEVGRLVTNIVFMGMGEPLYNLDAVVDAVGVMS
DQEGLGLSRRRITVSTSGVVPQIPRLGEQANAMLAISLHAVRDDLRDELVPLNRKYPIAELLAACRAYPGLSNARRITFE
YVMLKGVNDSDADARELVRLLKGIPAKINLIPFNPWPGSRYECSDWDRIERFSEIVFNAGYASPVRTPRGRDILAACGQL
KSETEKLRARARLMQEEGIGVESFYAGADD

Sequences:

>Translated_430_residues
MDRLNAMATPSLDSARAADAAIEKAPDLLPEALPGRRPSLVGLTRDALKAQLIGMGVPERESRMRAGQVWHWVNFRGASD
FAEMTNVGKALKAQLAEHFTLERPEVASRQVSRDGTRKWLLRMAPTNRQEHNRGAEIECVYIPGPDRGTLCVSSQVGCTL
TCSFCHTGTQRLVRNLSAAEIVQQLVTARDELGDWPGQMPSRDAGGSGEVGRLVTNIVFMGMGEPLYNLDAVVDAVGVMS
DQEGLGLSRRRITVSTSGVVPQIPRLGEQANAMLAISLHAVRDDLRDELVPLNRKYPIAELLAACRAYPGLSNARRITFE
YVMLKGVNDSDADARELVRLLKGIPAKINLIPFNPWPGSRYECSDWDRIERFSEIVFNAGYASPVRTPRGRDILAACGQL
KSETEKLRARARLMQEEGIGVESFYAGADD
>Mature_430_residues
MDRLNAMATPSLDSARAADAAIEKAPDLLPEALPGRRPSLVGLTRDALKAQLIGMGVPERESRMRAGQVWHWVNFRGASD
FAEMTNVGKALKAQLAEHFTLERPEVASRQVSRDGTRKWLLRMAPTNRQEHNRGAEIECVYIPGPDRGTLCVSSQVGCTL
TCSFCHTGTQRLVRNLSAAEIVQQLVTARDELGDWPGQMPSRDAGGSGEVGRLVTNIVFMGMGEPLYNLDAVVDAVGVMS
DQEGLGLSRRRITVSTSGVVPQIPRLGEQANAMLAISLHAVRDDLRDELVPLNRKYPIAELLAACRAYPGLSNARRITFE
YVMLKGVNDSDADARELVRLLKGIPAKINLIPFNPWPGSRYECSDWDRIERFSEIVFNAGYASPVRTPRGRDILAACGQL
KSETEKLRARARLMQEEGIGVESFYAGADD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=393, Percent_Identity=43.5114503816794, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 47225; Mature: 47225

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLNAMATPSLDSARAADAAIEKAPDLLPEALPGRRPSLVGLTRDALKAQLIGMGVPER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHCCCCCCH
ESRMRAGQVWHWVNFRGASDFAEMTNVGKALKAQLAEHFTLERPEVASRQVSRDGTRKWL
HHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
LRMAPTNRQEHNRGAEIECVYIPGPDRGTLCVSSQVGCTLTCSFCHTGTQRLVRNLSAAE
HEECCCCCHHCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHH
IVQQLVTARDELGDWPGQMPSRDAGGSGEVGRLVTNIVFMGMGEPLYNLDAVVDAVGVMS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
DQEGLGLSRRRITVSTSGVVPQIPRLGEQANAMLAISLHAVRDDLRDELVPLNRKYPIAE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHHH
LLAACRAYPGLSNARRITFEYVMLKGVNDSDADARELVRLLKGIPAKINLIPFNPWPGSR
HHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
YECSDWDRIERFSEIVFNAGYASPVRTPRGRDILAACGQLKSETEKLRARARLMQEEGIG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VESFYAGADD
HHHCCCCCCC
>Mature Secondary Structure
MDRLNAMATPSLDSARAADAAIEKAPDLLPEALPGRRPSLVGLTRDALKAQLIGMGVPER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHCCCCCCH
ESRMRAGQVWHWVNFRGASDFAEMTNVGKALKAQLAEHFTLERPEVASRQVSRDGTRKWL
HHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
LRMAPTNRQEHNRGAEIECVYIPGPDRGTLCVSSQVGCTLTCSFCHTGTQRLVRNLSAAE
HEECCCCCHHCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHH
IVQQLVTARDELGDWPGQMPSRDAGGSGEVGRLVTNIVFMGMGEPLYNLDAVVDAVGVMS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
DQEGLGLSRRRITVSTSGVVPQIPRLGEQANAMLAISLHAVRDDLRDELVPLNRKYPIAE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHHH
LLAACRAYPGLSNARRITFEYVMLKGVNDSDADARELVRLLKGIPAKINLIPFNPWPGSR
HHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
YECSDWDRIERFSEIVFNAGYASPVRTPRGRDILAACGQLKSETEKLRARARLMQEEGIG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VESFYAGADD
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA