Click here to switch to the map view.

The map label for this gene is yogA [H]

Identifier: 170747537

GI number: 170747537

Start: 1160679

End: 1161965

Strand: Reverse

Name: yogA [H]

Synonym: Mrad2831_1105

Alternate gene names: 170747537

Gene position: 1161965-1160679 (Counterclockwise)

Preceding gene: 170747539

Following gene: 170747536

Centisome position: 19.12

GC content: 67.99

Gene sequence:

>1287_bases
ATGGCGGCGAGCGCTGCGATCAGTCCGACGGGCGGGGAGGTCAAGGACCTCTACGAGATGGGTGAAATCCCGCCGCTGGG
TCATGTGCCGGCCAAGATGTATGCCTGGGCGATCCGGCGCGAGCGGCACGGACCCCCGGAGCAGTCCCACCAGCTCGAGG
TGCTGCCGGTCTGGGAGATCGGCGACGACGAGGTGCTGGTCTACGTCATGGCGGCGGGCGTCAACTACAACGGCGTCTGG
GCCGGCCTCGGCGAGCCGATCTCGCCCTTCGACGTCCACAAGGGCGAGTACCACATCGCGGGCTCCGACGCCTCCGGCAT
CGTCTGGAAGGTCGGCTCCAAGGTGAAGCGCTGGAAGGTCGGCGACGAGGTCATCGTCCACTGCAACCGGGACGACGGCG
ACGACGAGGAGTGCAATGGCGGCGACCCGATGCTGTCGCCCTCGCAGCGCATCTGGGGCTACGAGACCGGCGACGGCTCC
TTCGCGCAGTTCTGCCGGGTGCAGTCGCGTCAGCTCATGACGCGGCCCAAGCACCTCACCTGGGAGGAGGCCGCCTGCTA
CACGCTGACGCTCGCCACCGCCTACCGCATGCTGTTCGGCCACGCCCCGCACACGGTGAAGCCGGGCCAGAACGTGCTGA
TCTGGGGCGCCTCGGGCGGTCTCGGCGTGTTCGGCGTGCAGCTCTGCGCGGCCTCGGGCGCCAACGCCATCGCGGTGATC
TCGGACGAGTCGAAGCGCGACTACGTGATGAGCCTCGGCGCCAAGGGCGTCATCAACCGCAAGGACTTCGACTGCTGGGG
CCAGCTCCCGAAGGTCAACAGCCCCGAGTTCCACGCCTGGACCAAGGAGGCGCGGAAGTTCGGCAAGGCGATCTGGGACA
TCACCGGCAAGCAGGACGTCGACATCGTGTTCGAGCATCCCGGCGAGGCGACCTTCCCGGTCTCGGCGCTCGTGGTGAAG
CGCGGCGGCATGGTGGTGTTCTGCGCCGGTACGACCGGCTTCAACATCACCTTCGACGCCCGCTACGTCTGGATGCGGCA
GAAGCGCATCCAGGGCTCGCACTTCGCCCACCTCAAGCAGGCCTCGGCGGCGAACCAGTTCGTGCTCGACCAGCGGATCG
ACCCGTGCATGAGCGAGGTCTTCCCCTGGGACAAGATCCCCCAGGCCCACACCAAGATGTGGAAGAACCAGCACCCGCCG
GGCAACATGGCCTGCCTCGTCAACTCCCCGCGCGCCGGCCTGCGCACGGTCGAGGACGTGATCGAGGCCGGTCCGCTGAA
GCGGTAG

Upstream 100 bases:

>100_bases
GCTTTCGACTTTCGGCCCACTTGGACGCCTCCGGCATCGCCGCTATAGCGCGAAGGAGAACCGCGGCCGTGAGCAACACG
CGGGACGAGAGGAGAGCGAG

Downstream 100 bases:

>100_bases
GGCGCTCGATCGGACCGGCCCCGCCGCGCTCGCGCGGGGCCGGTCCGATCGGCTAAGCTCGCGGCGTCCCGCGCCGACTC
GCTGACCGGTTCGGTGTGCC

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAASAAISPTGGEVKDLYEMGEIPPLGHVPAKMYAWAIRRERHGPPEQSHQLEVLPVWEIGDDEVLVYVMAAGVNYNGVW
AGLGEPISPFDVHKGEYHIAGSDASGIVWKVGSKVKRWKVGDEVIVHCNRDDGDDEECNGGDPMLSPSQRIWGYETGDGS
FAQFCRVQSRQLMTRPKHLTWEEAACYTLTLATAYRMLFGHAPHTVKPGQNVLIWGASGGLGVFGVQLCAASGANAIAVI
SDESKRDYVMSLGAKGVINRKDFDCWGQLPKVNSPEFHAWTKEARKFGKAIWDITGKQDVDIVFEHPGEATFPVSALVVK
RGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASAANQFVLDQRIDPCMSEVFPWDKIPQAHTKMWKNQHPP
GNMACLVNSPRAGLRTVEDVIEAGPLKR

Sequences:

>Translated_428_residues
MAASAAISPTGGEVKDLYEMGEIPPLGHVPAKMYAWAIRRERHGPPEQSHQLEVLPVWEIGDDEVLVYVMAAGVNYNGVW
AGLGEPISPFDVHKGEYHIAGSDASGIVWKVGSKVKRWKVGDEVIVHCNRDDGDDEECNGGDPMLSPSQRIWGYETGDGS
FAQFCRVQSRQLMTRPKHLTWEEAACYTLTLATAYRMLFGHAPHTVKPGQNVLIWGASGGLGVFGVQLCAASGANAIAVI
SDESKRDYVMSLGAKGVINRKDFDCWGQLPKVNSPEFHAWTKEARKFGKAIWDITGKQDVDIVFEHPGEATFPVSALVVK
RGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASAANQFVLDQRIDPCMSEVFPWDKIPQAHTKMWKNQHPP
GNMACLVNSPRAGLRTVEDVIEAGPLKR
>Mature_427_residues
AASAAISPTGGEVKDLYEMGEIPPLGHVPAKMYAWAIRRERHGPPEQSHQLEVLPVWEIGDDEVLVYVMAAGVNYNGVWA
GLGEPISPFDVHKGEYHIAGSDASGIVWKVGSKVKRWKVGDEVIVHCNRDDGDDEECNGGDPMLSPSQRIWGYETGDGSF
AQFCRVQSRQLMTRPKHLTWEEAACYTLTLATAYRMLFGHAPHTVKPGQNVLIWGASGGLGVFGVQLCAASGANAIAVIS
DESKRDYVMSLGAKGVINRKDFDCWGQLPKVNSPEFHAWTKEARKFGKAIWDITGKQDVDIVFEHPGEATFPVSALVVKR
GGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASAANQFVLDQRIDPCMSEVFPWDKIPQAHTKMWKNQHPPG
NMACLVNSPRAGLRTVEDVIEAGPLKR

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=381, Percent_Identity=22.5721784776903, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI13236495, Length=381, Percent_Identity=22.5721784776903, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI194239676, Length=242, Percent_Identity=25.2066115702479, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790485, Length=370, Percent_Identity=22.972972972973, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=225, Percent_Identity=30.2222222222222, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=212, Percent_Identity=28.7735849056604, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=293, Percent_Identity=26.6211604095563, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=206, Percent_Identity=30.5825242718447, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=218, Percent_Identity=30.2752293577982, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=361, Percent_Identity=24.0997229916898, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 47185; Mature: 47054

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASAAISPTGGEVKDLYEMGEIPPLGHVPAKMYAWAIRRERHGPPEQSHQLEVLPVWEI
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
GDDEVLVYVMAAGVNYNGVWAGLGEPISPFDVHKGEYHIAGSDASGIVWKVGSKVKRWKV
CCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEC
GDEVIVHCNRDDGDDEECNGGDPMLSPSQRIWGYETGDGSFAQFCRVQSRQLMTRPKHLT
CCEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCEECCCCHHHHHHHHHHHHHHCCCCCCC
WEEAACYTLTLATAYRMLFGHAPHTVKPGQNVLIWGASGGLGVFGVQLCAASGANAIAVI
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEE
SDESKRDYVMSLGAKGVINRKDFDCWGQLPKVNSPEFHAWTKEARKFGKAIWDITGKQDV
ECCCCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
DIVFEHPGEATFPVSALVVKRGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQ
EEEEECCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEEEEEEEEHHHHCCCCHHHHHHH
ASAANQFVLDQRIDPCMSEVFPWDKIPQAHTKMWKNQHPPGNMACLVNSPRAGLRTVEDV
HHCCCHHHHHHHCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHH
IEAGPLKR
HHCCCCCC
>Mature Secondary Structure 
AASAAISPTGGEVKDLYEMGEIPPLGHVPAKMYAWAIRRERHGPPEQSHQLEVLPVWEI
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
GDDEVLVYVMAAGVNYNGVWAGLGEPISPFDVHKGEYHIAGSDASGIVWKVGSKVKRWKV
CCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEC
GDEVIVHCNRDDGDDEECNGGDPMLSPSQRIWGYETGDGSFAQFCRVQSRQLMTRPKHLT
CCEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCEECCCCHHHHHHHHHHHHHHCCCCCCC
WEEAACYTLTLATAYRMLFGHAPHTVKPGQNVLIWGASGGLGVFGVQLCAASGANAIAVI
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEE
SDESKRDYVMSLGAKGVINRKDFDCWGQLPKVNSPEFHAWTKEARKFGKAIWDITGKQDV
ECCCCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
DIVFEHPGEATFPVSALVVKRGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQ
EEEEECCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEEEEEEEEHHHHCCCCHHHHHHH
ASAANQFVLDQRIDPCMSEVFPWDKIPQAHTKMWKNQHPPGNMACLVNSPRAGLRTVEDV
HHCCCHHHHHHHCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHH
IEAGPLKR
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]