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The map label for this gene is shiA [H]

Identifier: 170747507

GI number: 170747507

Start: 1132439

End: 1133758

Strand: Reverse

Name: shiA [H]

Synonym: Mrad2831_1075

Alternate gene names: 170747507

Gene position: 1133758-1132439 (Counterclockwise)

Preceding gene: 170747508

Following gene: 170747506

Centisome position: 18.65

GC content: 71.29

Gene sequence:

>1320_bases
ATGGCCGTCACCACAGCCGCCCCGGCCGCCGGGCCCGGACAGAACCAGTCGGCGCGCGCGGCGCTGGCGGCCTTCGTGGG
CACCACGATCGAGTGGTACGACTTCTTCATCTACGGCACGGCGGCGGCCCTGGTGTTCGGGCCGCTCTTCTTCACGGCGG
AATCGCCCTACATCGCCACGCTCTCGGCCTTCGGCACCTTCGCGGTAGGCTTCCTGGCCCGGCCGCTCGGCGGCCTGATC
TTCGGGCATCTCGGCGACCGGTTCGGGCGCAAGCGCGCCCTGGTCGCGACGCTCGTGATGATGGGCATCGCCACCTCGTG
CATCGGGCTTCTGCCGACCTACGCCTCGGTCGGCCTGTGGGCGCCGGTGATGCTGGTGGTCCTGCGGCTGATCCAGGGGC
TCGCGGTCGGCGGCGAGTGGGGCGGGGCCGTGCTGATGGCCGCCGAGCACGCGCCGGCCGGGCGCAACACCTTCTTCGCC
TCCTTCGCGCAGCTCGGCAGTCCCGCCGGCCAGATCCTGTCGCTGCTGGCCTTCCGCCTCGCCGGCCTGCTCGACAAGGA
GAGCTTCCTGACCTGGGGCTGGCGGGTGCCGTTCCTGGTGAGCATCCTGCTGCTGTTCGTGGGGCTGGCGATCCGCCTCG
GGGTCGCGGAATCGCCGGCCTTCGAGAAGGCCCAGGCCGCGGGCGGACCGCCGCCGGCCCCCGTGCGCGAGGTGCTGACG
GCCTACCTCAAGCCAGTCCTGCTGGCGGCCGGCGCCAACACCTTCGCGATCGCCTCGGTCTACCTGTTCAACACCTTCAT
GATCGCGTTCACCACGCAGTATCTCGGCATCGCGCGGGCGACGATCCTCGACGCCCTGCTGATCGCCGCCGTGGTCCAGC
TGGTGATGACCCCGGTGGGCGCCCGGATCGCGGAGGTGATCCGCAACGAGCGGCTGTTCCTCCAGGGGACGCTGGTCTGG
GCGATGCTGGCCCCCTACCCGCTGTTCTGGCTGGTCGAGACCAAGTCGGTGCCGCTGATCGTGCTGGGCCTCGCCCTCAA
CGTCATCGGCAGCGCGACCTTCTACGCGGTGATCGCCGGCTACCTCGCGGGCGCCTTCCCGACGCGGGTGCGCTACACGG
CGATCTCCATCGCCTACCAGTTCTGCGGCGCCCTCGCCGGCGGCCTCACTCCGATCGTCGGCACGGTGCTGGCCGAGCGC
TTTCAGGGCCACTGGTGGCCGATCGCGGTGTTCGGGACGTGCCTCGCCGGCGTGTCGTTCCTGTGCGTCACGGCGATCGG
CGGCTACCGGGCCCGGCAGCGGGGCTTCCTCGACGGGTGA

Upstream 100 bases:

>100_bases
TGAACCATCCGACCGTGATCGCGCAGACGGTGCGGTTCCTTGCGACGGGGGCCTTCGCCGCGTAACCGTGAATTCAAAAT
ACCGTTACGCGCGAGGATCG

Downstream 100 bases:

>100_bases
GCGCGACCGGTCGAAGGCGCCGGATCCGGGTAACGCCGGATTTAGACCGGAGGTCTAGGGTCGGCGTCATGGATTCGATC
GCCGCAGCCGCCGTCCAGAT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAVTTAAPAAGPGQNQSARAALAAFVGTTIEWYDFFIYGTAAALVFGPLFFTAESPYIATLSAFGTFAVGFLARPLGGLI
FGHLGDRFGRKRALVATLVMMGIATSCIGLLPTYASVGLWAPVMLVVLRLIQGLAVGGEWGGAVLMAAEHAPAGRNTFFA
SFAQLGSPAGQILSLLAFRLAGLLDKESFLTWGWRVPFLVSILLLFVGLAIRLGVAESPAFEKAQAAGGPPPAPVREVLT
AYLKPVLLAAGANTFAIASVYLFNTFMIAFTTQYLGIARATILDALLIAAVVQLVMTPVGARIAEVIRNERLFLQGTLVW
AMLAPYPLFWLVETKSVPLIVLGLALNVIGSATFYAVIAGYLAGAFPTRVRYTAISIAYQFCGALAGGLTPIVGTVLAER
FQGHWWPIAVFGTCLAGVSFLCVTAIGGYRARQRGFLDG

Sequences:

>Translated_439_residues
MAVTTAAPAAGPGQNQSARAALAAFVGTTIEWYDFFIYGTAAALVFGPLFFTAESPYIATLSAFGTFAVGFLARPLGGLI
FGHLGDRFGRKRALVATLVMMGIATSCIGLLPTYASVGLWAPVMLVVLRLIQGLAVGGEWGGAVLMAAEHAPAGRNTFFA
SFAQLGSPAGQILSLLAFRLAGLLDKESFLTWGWRVPFLVSILLLFVGLAIRLGVAESPAFEKAQAAGGPPPAPVREVLT
AYLKPVLLAAGANTFAIASVYLFNTFMIAFTTQYLGIARATILDALLIAAVVQLVMTPVGARIAEVIRNERLFLQGTLVW
AMLAPYPLFWLVETKSVPLIVLGLALNVIGSATFYAVIAGYLAGAFPTRVRYTAISIAYQFCGALAGGLTPIVGTVLAER
FQGHWWPIAVFGTCLAGVSFLCVTAIGGYRARQRGFLDG
>Mature_438_residues
AVTTAAPAAGPGQNQSARAALAAFVGTTIEWYDFFIYGTAAALVFGPLFFTAESPYIATLSAFGTFAVGFLARPLGGLIF
GHLGDRFGRKRALVATLVMMGIATSCIGLLPTYASVGLWAPVMLVVLRLIQGLAVGGEWGGAVLMAAEHAPAGRNTFFAS
FAQLGSPAGQILSLLAFRLAGLLDKESFLTWGWRVPFLVSILLLFVGLAIRLGVAESPAFEKAQAAGGPPPAPVREVLTA
YLKPVLLAAGANTFAIASVYLFNTFMIAFTTQYLGIARATILDALLIAAVVQLVMTPVGARIAEVIRNERLFLQGTLVWA
MLAPYPLFWLVETKSVPLIVLGLALNVIGSATFYAVIAGYLAGAFPTRVRYTAISIAYQFCGALAGGLTPIVGTVLAERF
QGHWWPIAVFGTCLAGVSFLCVTAIGGYRARQRGFLDG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=38.9423076923077, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1790550, Length=399, Percent_Identity=32.3308270676692, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789941, Length=397, Percent_Identity=38.0352644836272, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788942, Length=391, Percent_Identity=30.1790281329923, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=25.6756756756757, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082404, Length=390, Percent_Identity=23.5897435897436, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46255; Mature: 46124

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTTAAPAAGPGQNQSARAALAAFVGTTIEWYDFFIYGTAAALVFGPLFFTAESPYIAT
CCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LSAFGTFAVGFLARPLGGLIFGHLGDRFGRKRALVATLVMMGIATSCIGLLPTYASVGLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APVMLVVLRLIQGLAVGGEWGGAVLMAAEHAPAGRNTFFASFAQLGSPAGQILSLLAFRL
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
AGLLDKESFLTWGWRVPFLVSILLLFVGLAIRLGVAESPAFEKAQAAGGPPPAPVREVLT
HHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHH
AYLKPVLLAAGANTFAIASVYLFNTFMIAFTTQYLGIARATILDALLIAAVVQLVMTPVG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ARIAEVIRNERLFLQGTLVWAMLAPYPLFWLVETKSVPLIVLGLALNVIGSATFYAVIAG
HHHHHHHHCCCCEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
YLAGAFPTRVRYTAISIAYQFCGALAGGLTPIVGTVLAERFQGHWWPIAVFGTCLAGVSF
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LCVTAIGGYRARQRGFLDG
HHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
AVTTAAPAAGPGQNQSARAALAAFVGTTIEWYDFFIYGTAAALVFGPLFFTAESPYIAT
CCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LSAFGTFAVGFLARPLGGLIFGHLGDRFGRKRALVATLVMMGIATSCIGLLPTYASVGLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APVMLVVLRLIQGLAVGGEWGGAVLMAAEHAPAGRNTFFASFAQLGSPAGQILSLLAFRL
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
AGLLDKESFLTWGWRVPFLVSILLLFVGLAIRLGVAESPAFEKAQAAGGPPPAPVREVLT
HHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHH
AYLKPVLLAAGANTFAIASVYLFNTFMIAFTTQYLGIARATILDALLIAAVVQLVMTPVG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ARIAEVIRNERLFLQGTLVWAMLAPYPLFWLVETKSVPLIVLGLALNVIGSATFYAVIAG
HHHHHHHHCCCCEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
YLAGAFPTRVRYTAISIAYQFCGALAGGLTPIVGTVLAERFQGHWWPIAVFGTCLAGVSF
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LCVTAIGGYRARQRGFLDG
HHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]