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The map label for this gene is yafJ [H]

Identifier: 170747466

GI number: 170747466

Start: 1088122

End: 1088907

Strand: Reverse

Name: yafJ [H]

Synonym: Mrad2831_1034

Alternate gene names: 170747466

Gene position: 1088907-1088122 (Counterclockwise)

Preceding gene: 170747467

Following gene: 170747465

Centisome position: 17.92

GC content: 70.48

Gene sequence:

>786_bases
ATGTGCGAACTGCTCGGCATGAGCGCCAACGTGCCGACCGACATCCGCTTCTCCTTCGCGGGGCTCGCCCGGCGCGGCGG
CGAGACCGGGCCCCACGCCGACGGCTGGGGCATCAGCTTCTACGACGGCCGCACCTGCCGCAGCTTCCACGAGCCGGAGC
CGAGCGCCCGCTCGCAGCTCGCCCGGCTCCTGCGCGACATGCCGATCAAGAGCCGGATCGTCGTCGCCCATGTCCGCAAG
GCCAATCGCGGCCGGGTCAGCCTCGAGAACACCCACCCGTTCTCCCGGGAATTGTGGGGCCGCCGCTGGACCTTCGCGCA
CAACGGCCAGCTCAAGGGCGTGAAGCGGCTGCCGCTGGGCGGCTTCATGCCGATCGGGTCCACCGACAGCGAGCACGCCT
TCTGCTGGATGCTTGGCCGGCTCCAGGCCCGGTTCCGCGCCCTGCCCCGGCCCGAGACCCTCGACCGGGCGGTGGCCGAC
CTCGCGGGCGAGCTGCACGCGCTCGGCGTGTTCAACATGCTGCTCACCGACAGCCGCACCCTCTACGCCCATTGCGGCAA
GCGCCTCTGCTACCTCACCCGCTGCGCGCCCTTCGGGAAGGCCACCCTGATCGACGAGGACTGGCAGGTGGATTTCGCCC
AGGAGACCACCGAGCACGACGTGGTGACGGTCATCGCCACGCAGGCGCTGACCCGGGACGAGTGCTGGACCGATCTCGCC
CGCGGCGACGTCCTGACCCTGCGCGACGGGGCGATCCGGCTGTTGAGACCGAGCAACTTAGCTTAA

Upstream 100 bases:

>100_bases
GACCGACTATCTCGCCCGCTGACCCGCGCGAAAGCCGTTGTCGGGGCGGCCGAACGCTCCTAAACGTCGCCGCTTCCCCG
CATGGCCCGGCTGCAAGACG

Downstream 100 bases:

>100_bases
GTGTGTTTTGCCTGAGGGTGCATGCCCGCGCCCGATGCGCTAGAACTCCGGTAACAGGAGCGCATCGGCCGGACTTCCGC
ACCGGGTCGAGCCGGCAAGC

Product: glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MCELLGMSANVPTDIRFSFAGLARRGGETGPHADGWGISFYDGRTCRSFHEPEPSARSQLARLLRDMPIKSRIVVAHVRK
ANRGRVSLENTHPFSRELWGRRWTFAHNGQLKGVKRLPLGGFMPIGSTDSEHAFCWMLGRLQARFRALPRPETLDRAVAD
LAGELHALGVFNMLLTDSRTLYAHCGKRLCYLTRCAPFGKATLIDEDWQVDFAQETTEHDVVTVIATQALTRDECWTDLA
RGDVLTLRDGAIRLLRPSNLA

Sequences:

>Translated_261_residues
MCELLGMSANVPTDIRFSFAGLARRGGETGPHADGWGISFYDGRTCRSFHEPEPSARSQLARLLRDMPIKSRIVVAHVRK
ANRGRVSLENTHPFSRELWGRRWTFAHNGQLKGVKRLPLGGFMPIGSTDSEHAFCWMLGRLQARFRALPRPETLDRAVAD
LAGELHALGVFNMLLTDSRTLYAHCGKRLCYLTRCAPFGKATLIDEDWQVDFAQETTEHDVVTVIATQALTRDECWTDLA
RGDVLTLRDGAIRLLRPSNLA
>Mature_261_residues
MCELLGMSANVPTDIRFSFAGLARRGGETGPHADGWGISFYDGRTCRSFHEPEPSARSQLARLLRDMPIKSRIVVAHVRK
ANRGRVSLENTHPFSRELWGRRWTFAHNGQLKGVKRLPLGGFMPIGSTDSEHAFCWMLGRLQARFRALPRPETLDRAVAD
LAGELHALGVFNMLLTDSRTLYAHCGKRLCYLTRCAPFGKATLIDEDWQVDFAQETTEHDVVTVIATQALTRDECWTDLA
RGDVLTLRDGAIRLLRPSNLA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=244, Percent_Identity=59.4262295081967, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29258; Mature: 29258

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDIRFSFAGLARRGGETGPHADGWGISFYDGRTCRSFHEPEPSARSQL
CCCEECCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHCCCCCCHHHHHHH
ARLLRDMPIKSRIVVAHVRKANRGRVSLENTHPFSRELWGRRWTFAHNGQLKGVKRLPLG
HHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHCCCEEEEEECCCCCCEEECCCC
GFMPIGSTDSEHAFCWMLGRLQARFRALPRPETLDRAVADLAGELHALGVFNMLLTDSRT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LYAHCGKRLCYLTRCAPFGKATLIDEDWQVDFAQETTEHDVVTVIATQALTRDECWTDLA
HHHHHCHHHHHHHHCCCCCCEEEECCCCCEEHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
RGDVLTLRDGAIRLLRPSNLA
CCCEEEEECCCEEEECCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDIRFSFAGLARRGGETGPHADGWGISFYDGRTCRSFHEPEPSARSQL
CCCEECCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHCCCCCCHHHHHHH
ARLLRDMPIKSRIVVAHVRKANRGRVSLENTHPFSRELWGRRWTFAHNGQLKGVKRLPLG
HHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHCCCEEEEEECCCCCCEEECCCC
GFMPIGSTDSEHAFCWMLGRLQARFRALPRPETLDRAVADLAGELHALGVFNMLLTDSRT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LYAHCGKRLCYLTRCAPFGKATLIDEDWQVDFAQETTEHDVVTVIATQALTRDECWTDLA
HHHHHCHHHHHHHHCCCCCCEEEECCCCCEEHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
RGDVLTLRDGAIRLLRPSNLA
CCCEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]