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The map label for this gene is potF [H]

Identifier: 170747368

GI number: 170747368

Start: 981791

End: 982918

Strand: Reverse

Name: potF [H]

Synonym: Mrad2831_0935

Alternate gene names: 170747368

Gene position: 982918-981791 (Counterclockwise)

Preceding gene: 170747369

Following gene: 170747361

Centisome position: 16.17

GC content: 67.73

Gene sequence:

>1128_bases
GTGAACGGCGCGCGCCGGACCGTCCTGGCGGCCGCTGCAGCCGTTCTCGCGGGAGGGCTCCTGGCGGCCCTGCCCCTCGT
GCGGCCGGCACCGGCCGCCGAGGAGCGGGTCGTCAACATCTACAACTGGTCGGACTATATCGACCCCACGGTGCTCGAGG
CCTTCACCCGCGAGACCGGGATCAAGGTCGTCTACGACACCTACGACAACAACGAGATCTTGGAGACCAAGCTGCTCGCC
GGCCGGTCGGGCTACGACATCGTCGTGCCGTCAGGGCCCTACCTGCAGCGCCTGATCCGGGCCGGCGCCTTCCTGCCCCT
CGACAAGTCCAGGCTGAAGAACCTCGGCAATCTCTGGCCCGAGGTGACCAGCCGGCTGGCGGCCTACGATCCCGGCAACA
CCTACGCCGTCGACTACATGTGGGGCACGACCGGCATCGGCTACAACGTCGCCGCCGTCCGCGAGCGCCTGGGCGCCAAC
GCGACGGTGAACTCGTGGTCCGTCGTCCTCAACCCCGCCTCGGCGAACAAGCTGAAGGAGTGCGGGATCATGATGCTCGA
CAGCCCCGAGGACCTGATCCCCAGCATGCTCCCGGCCTTCGGCGCGAAGGCGGATTCCAAGCGCTGGGACGACATGACCC
TGGTGACCGACGCCCTCTACAAGGTGCGCGGCGCCGTGCGGAAGTTCCACTCGTCGGAGTACATCCAGGCGCTGGCGAAC
GGCGATATCTGCCTGGCCGTCGGCTACTCGGGCGACATCATGCAGGCGAAGCGCCGCGCCGAGGAGGCCAAGAACGGGAT
CGACATCGCCTACGTCGTGCCGAAGGAGGGCGCGCTGATGTGGTTCGACACCTTCGCGATCCCGAAGGATGCCGCCCACC
CGGCCGAGGCGCTGGCCTTCATCGACTACATGATGCGGCCCGAGGTGGCGGCGGCCAACACCAACTTCGTCTCCTACGCC
AGCGGCAACCTCGCGGCGAAGAAGTTCGTGAAGCCCGAGATCCTGAGCAATCCCGGCATCTACCCGGACGAGGCGACGAT
GCAGCGCCTGTCCATCAACACCGCCTGGGACGACCGCACCCAGCGCTTCGTCACCCGCCTCTGGACTCGGGTCCGCACCG
GGCGCTGA

Upstream 100 bases:

>100_bases
GGGTCTCCCCGGAGAACCCGCCGGCCCGCGCCACGGTCGAGGCCAAGCTCGCCGCGCCCGAGTACCTGGTCGAGATCGTG
GTCGTGGCCGCGAGGAGCCG

Downstream 100 bases:

>100_bases
CGCGGAGCCCGGCCGGGTCTCGTCAGCGCCCCGCCTCCGCGGGGACGCCGTGCCGGACGAGCCACGCGCGGACCGCCGCG
CGGGTCTCGCGCGCTTCGTC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNGARRTVLAAAAAVLAGGLLAALPLVRPAPAAEERVVNIYNWSDYIDPTVLEAFTRETGIKVVYDTYDNNEILETKLLA
GRSGYDIVVPSGPYLQRLIRAGAFLPLDKSRLKNLGNLWPEVTSRLAAYDPGNTYAVDYMWGTTGIGYNVAAVRERLGAN
ATVNSWSVVLNPASANKLKECGIMMLDSPEDLIPSMLPAFGAKADSKRWDDMTLVTDALYKVRGAVRKFHSSEYIQALAN
GDICLAVGYSGDIMQAKRRAEEAKNGIDIAYVVPKEGALMWFDTFAIPKDAAHPAEALAFIDYMMRPEVAAANTNFVSYA
SGNLAAKKFVKPEILSNPGIYPDEATMQRLSINTAWDDRTQRFVTRLWTRVRTGR

Sequences:

>Translated_375_residues
MNGARRTVLAAAAAVLAGGLLAALPLVRPAPAAEERVVNIYNWSDYIDPTVLEAFTRETGIKVVYDTYDNNEILETKLLA
GRSGYDIVVPSGPYLQRLIRAGAFLPLDKSRLKNLGNLWPEVTSRLAAYDPGNTYAVDYMWGTTGIGYNVAAVRERLGAN
ATVNSWSVVLNPASANKLKECGIMMLDSPEDLIPSMLPAFGAKADSKRWDDMTLVTDALYKVRGAVRKFHSSEYIQALAN
GDICLAVGYSGDIMQAKRRAEEAKNGIDIAYVVPKEGALMWFDTFAIPKDAAHPAEALAFIDYMMRPEVAAANTNFVSYA
SGNLAAKKFVKPEILSNPGIYPDEATMQRLSINTAWDDRTQRFVTRLWTRVRTGR
>Mature_375_residues
MNGARRTVLAAAAAVLAGGLLAALPLVRPAPAAEERVVNIYNWSDYIDPTVLEAFTRETGIKVVYDTYDNNEILETKLLA
GRSGYDIVVPSGPYLQRLIRAGAFLPLDKSRLKNLGNLWPEVTSRLAAYDPGNTYAVDYMWGTTGIGYNVAAVRERLGAN
ATVNSWSVVLNPASANKLKECGIMMLDSPEDLIPSMLPAFGAKADSKRWDDMTLVTDALYKVRGAVRKFHSSEYIQALAN
GDICLAVGYSGDIMQAKRRAEEAKNGIDIAYVVPKEGALMWFDTFAIPKDAAHPAEALAFIDYMMRPEVAAANTNFVSYA
SGNLAAKKFVKPEILSNPGIYPDEATMQRLSINTAWDDRTQRFVTRLWTRVRTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=345, Percent_Identity=48.695652173913, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1787367, Length=350, Percent_Identity=30.5714285714286, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41184; Mature: 41184

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGARRTVLAAAAAVLAGGLLAALPLVRPAPAAEERVVNIYNWSDYIDPTVLEAFTRETG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCEEEEECCCCCCCHHHHHHHHHCCC
IKVVYDTYDNNEILETKLLAGRSGYDIVVPSGPYLQRLIRAGAFLPLDKSRLKNLGNLWP
CEEEEEECCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
EVTSRLAAYDPGNTYAVDYMWGTTGIGYNVAAVRERLGANATVNSWSVVLNPASANKLKE
HHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEECCEEEEECCCCHHHHHH
CGIMMLDSPEDLIPSMLPAFGAKADSKRWDDMTLVTDALYKVRGAVRKFHSSEYIQALAN
CCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDICLAVGYSGDIMQAKRRAEEAKNGIDIAYVVPKEGALMWFDTFAIPKDAAHPAEALAF
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHH
IDYMMRPEVAAANTNFVSYASGNLAAKKFVKPEILSNPGIYPDEATMQRLSINTAWDDRT
HHHHHCCCCEECCCCEEEECCCCHHHHHHCCCHHHCCCCCCCCHHHHHHEECCCCCCHHH
QRFVTRLWTRVRTGR
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGARRTVLAAAAAVLAGGLLAALPLVRPAPAAEERVVNIYNWSDYIDPTVLEAFTRETG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCEEEEECCCCCCCHHHHHHHHHCCC
IKVVYDTYDNNEILETKLLAGRSGYDIVVPSGPYLQRLIRAGAFLPLDKSRLKNLGNLWP
CEEEEEECCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
EVTSRLAAYDPGNTYAVDYMWGTTGIGYNVAAVRERLGANATVNSWSVVLNPASANKLKE
HHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEECCEEEEECCCCHHHHHH
CGIMMLDSPEDLIPSMLPAFGAKADSKRWDDMTLVTDALYKVRGAVRKFHSSEYIQALAN
CCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDICLAVGYSGDIMQAKRRAEEAKNGIDIAYVVPKEGALMWFDTFAIPKDAAHPAEALAF
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHH
IDYMMRPEVAAANTNFVSYASGNLAAKKFVKPEILSNPGIYPDEATMQRLSINTAWDDRT
HHHHHCCCCEECCCCEEEECCCCHHHHHHCCCHHHCCCCCCCCHHHHHHEECCCCCCHHH
QRFVTRLWTRVRTGR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]