| Definition | Methylobacterium radiotolerans JCM 2831 chromosome, complete genome. |
|---|---|
| Accession | NC_010505 |
| Length | 6,077,833 |
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The map label for this gene is mrcB [H]
Identifier: 170747311
GI number: 170747311
Start: 921098
End: 923392
Strand: Reverse
Name: mrcB [H]
Synonym: Mrad2831_0878
Alternate gene names: 170747311
Gene position: 923392-921098 (Counterclockwise)
Preceding gene: 170747313
Following gene: 170747310
Centisome position: 15.19
GC content: 69.5
Gene sequence:
>2295_bases GTGCGCCTGCCGACGTTCACCGAGATCAAGGTCCGCCTCGAACGCGCCGCCCTCGCCTTCGACGCCTGGGTCAACGCGAG CCTGTACGACGGCGGCCAGTCGACCGGTCAGGCCTACGAGCGCTTCCAGCGCGTCATGAGCCGCTTCGCGGTGCGCGGCT GGAAGCGCGCCGCCCTCGACCTGACGAGCGAGGGCGTCACCATCGGGACCGGCGGCGCCATCCTGATGCTCGCCCTGGCG CAGCCCGCCTTCCAGCTCACCAGCGAGAACTGGCTGAAGCAGCAGGACCTCGCCGTCACCTTCCTGGACCGTTACGGCAC CGAGGTCGGGCGCCGGGGCATCAAGCACGACGACTCGCTGAAGCTCGACGAGTTTCCCGACATCATGATCAAGGCGCTGG TCTCCACGGAGGACCGGCGCTTCTACGAGCACTGGGGCATCGACCCGATCGGCACGCTGCGGGCGCTGGTGAACAATTCC CGCGGCGGCAGCGGCACGCAGGGCGGGTCCTCGATCACCCAGCAGCTCGCCAAGAACCTGTTCCTGACGAACGAGCGCTC CCTGGAGCGGAAGATCAACGAGGCCTTCCTGGCCCTGTGGCTCGAGAGCCATCTCACCAAGAACGAGATCCTGAAGCTCT ACCTCGACCGCGCGTATATGGGCGGTGGAACCTTCGGCGCGGTGGCGGCCGCGGATTACTATTTCGGCAAGCCGCTCAAG GACATCAGCCTTGCGGAGGCCGCCATGCTGGCGGGCCTGTTCAAGGCGCCGACCAAGTACGCCCCGCACGTCAACCTGCC GGCGGCCCGCGCCCGGGCGGCGGACGTGCTGCACAACATGGTGGAGGCGGGCTTCGTCACCGAGGGCCAGATCCAGACGG CGCTGCGCAACCCGGCGACGCCGGTGACCCGCACCCGCGACATCACCGCCGACTACTACCTCGACTGGGCCTTCGGCGAG ATCAAGGCGATGGCCGACGCCGGCAAGCTGCGCAACGACCGCGTGCTGACCGTGAAGACGCCGCTCGACCTCGCGATCCA GAAGCGCGCCGACGAGGTGGTGGCCGACATCCTGCGCAAGTCCGGCGACGCCTACGATGTGGACGAGGCCGCCATGGTGA TCCTCGATCCCGACGGGGCGCTGCGCGCCATGGTGGGCGGCGCCGATTACGGCGAGAGCCAGTTCAACCGTGCCACCGAC GCGCTGCGCCAGCCCGGCTCCTCGTTCAAGCCCTACGTCTACTCGGCCGCCCTGAACTCGGGCCTGTTCAAGCCCGACAC GACCGTGGTCGACAGCCCCGTCTGCATCGGCAACTGGTGCCCGCAGAACTACGGCCGCTCCTATGCCGGCCGGGTGCCGA TGTGGCTCGCGGTGGCCAAGTCGATCAACACGATCCCGATCAAGATCTCCATCGCCCTCGGCAAGGCGATGGGCATCACC CACGAGGCGAAGGCCGCGAAGGCGGGCCGCGCGAAGATCATCGAGCTCGCCCACAAGATGGGCATCACCTCGAACCTGAT CGACACGGTCTCGATGCCGATCGGCTCGGACGAGGTGACGGTCATCGACCAGGCGGCGGGCTACGCGGTGTTCGCCAACG GCGGCTTCCGCGCCAAGCCCTACGCGGCCATCGAGGTGAAGAACTCGTCCGGCGAGGTGATCTACCGCCACGCCGACGAG GCGCCCGAGCGGGTGCTCGCGCCCCAGGTCGTGGCGGACATGAACTTCATGCTCAACAAGGTCGTCGAGGAGGGCACCGG CAAGCGGGCGCAGCTCGAGGGCGTGAAGGTGGCCGGCAAGTCCGGCACGACGAACGCCTACCGGGACGCGTGGTTCGTCG GCTACACCGGCAATTACGTCGGCGCCGTCTGGTTCGGCAACGACGACCACACCTCGACCAACAAGCTCACCGGCGGCTCC CTCCCGGCCCAGCTCTGGCACGACGTGATGGAGCCGGCCCACCAGGGCATCGAGATCAAGGGTCTGCCCGGCCTGAAGGA GAGCCCGCGCGCCGCCCAGCAGCAGACCTCCGGCGGCCCGACCGCCCCGACCGATCCCAATGCCAGCGCGTACGGCAAGC TCTCCCGCCGCTCCTTCGAGGTGATCAGCGGCCTCAACGGCCTGTTCCGCACCGTCGAGCGCGCCCCCGGATCCGCCGCG GACGCGCCCGCCAACGCCGGGGACGCGCCGAAGACGGGCCGGAAGCCCGGCGACCGGGCGGCGGCGCCGTCGGGTCAGCT GCGCGAGGTCGCCGAGGGCGCCCGCCCGGTCGGCGGCTTCACCGAGGTCCGGTGA
Upstream 100 bases:
>100_bases GCGGCGGCGCCGGCGCAGGGTTCGTCTCCGCGGCGCGGGCGCCCGACCATCCGCCGCCAGGATCCGCCGCAGAGGCGCTC GACAAGCCGGGTTCGACATC
Downstream 100 bases:
>100_bases GCGCATGAAGCTCACGATCCCCGACGCGTTCGGCCGGACCGCCGCGCTCGCCGGCGCCCCGCGCGGCGGCGCTGCCCGCC TCGTCCTGCGCCTCTGGCGC
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 764; Mature: 764
Protein sequence:
>764_residues MRLPTFTEIKVRLERAALAFDAWVNASLYDGGQSTGQAYERFQRVMSRFAVRGWKRAALDLTSEGVTIGTGGAILMLALA QPAFQLTSENWLKQQDLAVTFLDRYGTEVGRRGIKHDDSLKLDEFPDIMIKALVSTEDRRFYEHWGIDPIGTLRALVNNS RGGSGTQGGSSITQQLAKNLFLTNERSLERKINEAFLALWLESHLTKNEILKLYLDRAYMGGGTFGAVAAADYYFGKPLK DISLAEAAMLAGLFKAPTKYAPHVNLPAARARAADVLHNMVEAGFVTEGQIQTALRNPATPVTRTRDITADYYLDWAFGE IKAMADAGKLRNDRVLTVKTPLDLAIQKRADEVVADILRKSGDAYDVDEAAMVILDPDGALRAMVGGADYGESQFNRATD ALRQPGSSFKPYVYSAALNSGLFKPDTTVVDSPVCIGNWCPQNYGRSYAGRVPMWLAVAKSINTIPIKISIALGKAMGIT HEAKAAKAGRAKIIELAHKMGITSNLIDTVSMPIGSDEVTVIDQAAGYAVFANGGFRAKPYAAIEVKNSSGEVIYRHADE APERVLAPQVVADMNFMLNKVVEEGTGKRAQLEGVKVAGKSGTTNAYRDAWFVGYTGNYVGAVWFGNDDHTSTNKLTGGS LPAQLWHDVMEPAHQGIEIKGLPGLKESPRAAQQQTSGGPTAPTDPNASAYGKLSRRSFEVISGLNGLFRTVERAPGSAA DAPANAGDAPKTGRKPGDRAAAPSGQLREVAEGARPVGGFTEVR
Sequences:
>Translated_764_residues MRLPTFTEIKVRLERAALAFDAWVNASLYDGGQSTGQAYERFQRVMSRFAVRGWKRAALDLTSEGVTIGTGGAILMLALA QPAFQLTSENWLKQQDLAVTFLDRYGTEVGRRGIKHDDSLKLDEFPDIMIKALVSTEDRRFYEHWGIDPIGTLRALVNNS RGGSGTQGGSSITQQLAKNLFLTNERSLERKINEAFLALWLESHLTKNEILKLYLDRAYMGGGTFGAVAAADYYFGKPLK DISLAEAAMLAGLFKAPTKYAPHVNLPAARARAADVLHNMVEAGFVTEGQIQTALRNPATPVTRTRDITADYYLDWAFGE IKAMADAGKLRNDRVLTVKTPLDLAIQKRADEVVADILRKSGDAYDVDEAAMVILDPDGALRAMVGGADYGESQFNRATD ALRQPGSSFKPYVYSAALNSGLFKPDTTVVDSPVCIGNWCPQNYGRSYAGRVPMWLAVAKSINTIPIKISIALGKAMGIT HEAKAAKAGRAKIIELAHKMGITSNLIDTVSMPIGSDEVTVIDQAAGYAVFANGGFRAKPYAAIEVKNSSGEVIYRHADE APERVLAPQVVADMNFMLNKVVEEGTGKRAQLEGVKVAGKSGTTNAYRDAWFVGYTGNYVGAVWFGNDDHTSTNKLTGGS LPAQLWHDVMEPAHQGIEIKGLPGLKESPRAAQQQTSGGPTAPTDPNASAYGKLSRRSFEVISGLNGLFRTVERAPGSAA DAPANAGDAPKTGRKPGDRAAAPSGQLREVAEGARPVGGFTEVR >Mature_764_residues MRLPTFTEIKVRLERAALAFDAWVNASLYDGGQSTGQAYERFQRVMSRFAVRGWKRAALDLTSEGVTIGTGGAILMLALA QPAFQLTSENWLKQQDLAVTFLDRYGTEVGRRGIKHDDSLKLDEFPDIMIKALVSTEDRRFYEHWGIDPIGTLRALVNNS RGGSGTQGGSSITQQLAKNLFLTNERSLERKINEAFLALWLESHLTKNEILKLYLDRAYMGGGTFGAVAAADYYFGKPLK DISLAEAAMLAGLFKAPTKYAPHVNLPAARARAADVLHNMVEAGFVTEGQIQTALRNPATPVTRTRDITADYYLDWAFGE IKAMADAGKLRNDRVLTVKTPLDLAIQKRADEVVADILRKSGDAYDVDEAAMVILDPDGALRAMVGGADYGESQFNRATD ALRQPGSSFKPYVYSAALNSGLFKPDTTVVDSPVCIGNWCPQNYGRSYAGRVPMWLAVAKSINTIPIKISIALGKAMGIT HEAKAAKAGRAKIIELAHKMGITSNLIDTVSMPIGSDEVTVIDQAAGYAVFANGGFRAKPYAAIEVKNSSGEVIYRHADE APERVLAPQVVADMNFMLNKVVEEGTGKRAQLEGVKVAGKSGTTNAYRDAWFVGYTGNYVGAVWFGNDDHTSTNKLTGGS LPAQLWHDVMEPAHQGIEIKGLPGLKESPRAAQQQTSGGPTAPTDPNASAYGKLSRRSFEVISGLNGLFRTVERAPGSAA DAPANAGDAPKTGRKPGDRAAAPSGQLREVAEGARPVGGFTEVR
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=550, Percent_Identity=31.4545454545455, Blast_Score=226, Evalue=4e-60, Organism=Escherichia coli, GI1788867, Length=509, Percent_Identity=28.2907662082515, Blast_Score=177, Evalue=2e-45, Organism=Escherichia coli, GI87082258, Length=208, Percent_Identity=43.75, Blast_Score=174, Evalue=3e-44, Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=37.7049180327869, Blast_Score=120, Evalue=4e-28,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 82367; Mature: 82367
Theoretical pI: Translated: 8.90; Mature: 8.90
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLPTFTEIKVRLERAALAFDAWVNASLYDGGQSTGQAYERFQRVMSRFAVRGWKRAALD CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHE LTSEGVTIGTGGAILMLALAQPAFQLTSENWLKQQDLAVTFLDRYGTEVGRRGIKHDDSL ECCCCEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCC KLDEFPDIMIKALVSTEDRRFYEHWGIDPIGTLRALVNNSRGGSGTQGGSSITQQLAKNL CCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH FLTNERSLERKINEAFLALWLESHLTKNEILKLYLDRAYMGGGTFGAVAAADYYFGKPLK CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC DISLAEAAMLAGLFKAPTKYAPHVNLPAARARAADVLHNMVEAGFVTEGQIQTALRNPAT HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC PVTRTRDITADYYLDWAFGEIKAMADAGKLRNDRVLTVKTPLDLAIQKRADEVVADILRK CCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC SGDAYDVDEAAMVILDPDGALRAMVGGADYGESQFNRATDALRQPGSSFKPYVYSAALNS CCCCCCCCCCEEEEECCCCCEEHEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHC GLFKPDTTVVDSPVCIGNWCPQNYGRSYAGRVPMWLAVAKSINTIPIKISIALGKAMGIT CCCCCCCCEECCCCEECCCCCHHHCCCCCCCCHHHHHHHHCCCEEEEEEEEEECHHHCCC HEAKAAKAGRAKIIELAHKMGITSNLIDTVSMPIGSDEVTVIDQAAGYAVFANGGFRAKP CHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCC YAAIEVKNSSGEVIYRHADEAPERVLAPQVVADMNFMLNKVVEEGTGKRAQLEGVKVAGK EEEEEEECCCCCEEEECCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECC SGTTNAYRDAWFVGYTGNYVGAVWFGNDDHTSTNKLTGGSLPAQLWHDVMEPAHQGIEIK CCCCCCHHCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEE GLPGLKESPRAAQQQTSGGPTAPTDPNASAYGKLSRRSFEVISGLNGLFRTVERAPGSAA CCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC DAPANAGDAPKTGRKPGDRAAAPSGQLREVAEGARPVGGFTEVR CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC >Mature Secondary Structure MRLPTFTEIKVRLERAALAFDAWVNASLYDGGQSTGQAYERFQRVMSRFAVRGWKRAALD CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHE LTSEGVTIGTGGAILMLALAQPAFQLTSENWLKQQDLAVTFLDRYGTEVGRRGIKHDDSL ECCCCEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCC KLDEFPDIMIKALVSTEDRRFYEHWGIDPIGTLRALVNNSRGGSGTQGGSSITQQLAKNL CCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH FLTNERSLERKINEAFLALWLESHLTKNEILKLYLDRAYMGGGTFGAVAAADYYFGKPLK CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC DISLAEAAMLAGLFKAPTKYAPHVNLPAARARAADVLHNMVEAGFVTEGQIQTALRNPAT HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC PVTRTRDITADYYLDWAFGEIKAMADAGKLRNDRVLTVKTPLDLAIQKRADEVVADILRK CCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC SGDAYDVDEAAMVILDPDGALRAMVGGADYGESQFNRATDALRQPGSSFKPYVYSAALNS CCCCCCCCCCEEEEECCCCCEEHEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHC GLFKPDTTVVDSPVCIGNWCPQNYGRSYAGRVPMWLAVAKSINTIPIKISIALGKAMGIT CCCCCCCCEECCCCEECCCCCHHHCCCCCCCCHHHHHHHHCCCEEEEEEEEEECHHHCCC HEAKAAKAGRAKIIELAHKMGITSNLIDTVSMPIGSDEVTVIDQAAGYAVFANGGFRAKP CHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCC YAAIEVKNSSGEVIYRHADEAPERVLAPQVVADMNFMLNKVVEEGTGKRAQLEGVKVAGK EEEEEEECCCCCEEEECCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECC SGTTNAYRDAWFVGYTGNYVGAVWFGNDDHTSTNKLTGGSLPAQLWHDVMEPAHQGIEIK CCCCCCHHCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEE GLPGLKESPRAAQQQTSGGPTAPTDPNASAYGKLSRRSFEVISGLNGLFRTVERAPGSAA CCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC DAPANAGDAPKTGRKPGDRAAAPSGQLREVAEGARPVGGFTEVR CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]