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The map label for this gene is gabT [C]

Identifier: 170747281

GI number: 170747281

Start: 890341

End: 891729

Strand: Reverse

Name: gabT [C]

Synonym: Mrad2831_0848

Alternate gene names: 170747281

Gene position: 891729-890341 (Counterclockwise)

Preceding gene: 170747283

Following gene: 170747278

Centisome position: 14.67

GC content: 71.42

Gene sequence:

>1389_bases
ATGCTCTCGAACCTCGCGACCCGCGACGTCGAAACCCTCCTGCATCCCTACACCAACCTGTCGGCGCACCGACAGACGGG
CCCGCTGGTTCTCGAGCGCGGCGAGGGCGTGCACGTCTACGACACCGAGGGGCGCTCCTACATCGAGGGCATGTCCGGCC
TGTGGTGCACGGCCCTCGGCTACTCGAACGCGGAGCTCGTGGAGGCAGCCCGGGCGCAGATGGCGCGCCTGCCCTTCACG
CACCTGTTCTCCGGCCGCAGCCACGATCCGGGCATCGAGCTCGCCGAGACGCTCAAGGAGCTGATGCCGGTCCCGACCTC
GAAGATCTTCTTCACCTCCTCGGGGTCGGAGGCCAACGACACGCAGGTCAAGCTGACTTGGTACTACAACAACGCCCTGG
GCCGGCCGAAGAAGAAGAAGATCATCGCCCGCCAGAAGGGCTACCACGGCGTCACGGTAGCCAGCGCCTCGATGACCGGG
CTCGCGGCGAACCACGCCGACTGGGACCTGCCGCTGCCGGGCTTCCTGCACGTCTCGCCCGCGCACCACTACCGGGGCGC
GGAGCCGGGCGAGAGCGAGGAGGCCTACTCGGCGCGCCTCGCCGCCGAGCTGGAGGAGACGATCCTGCGCGAGGGCCCCG
ACACCGTCGCGGCCTTCATCGCCGAGCCGGTGATGGGCGCGGGCGGCGCGATCGTGCCGCCGCGGGGCTACTTCTCGGCG
ATCCAGGCGGTGCTGGCGAAGTACGACGTCCGCTTCATCGCCGACGAGGTGATCTGCGGCTTCGGCCGGCTCGGCACGTG
GTTCGGCAGCGAGGCGGTGGGGGCCCGGCCCGACTCTATCTCCTTCGCCAAGGCCCTGACCTCGGCCTACATGCCGCTCG
GCGGCGTCAGCATCGACGAGCCCCTCTACGAGGCGCTGATCGGACAGAGCGAGAAGATCGGCACCTTCGGCCACGGCACC
ACCTATTCCGGGCACCCGGTGGCCGCCGCGGTGGCGCTGAAGGCGATCGAGATCTACCGCCGCGAGCGGGTGATCGAGGG
GGCGGCCGAGAAGGCGCCCCAGTTCCAGCGCCGTCTCGCGGCCCTCGCCGGGCACGACATCGTGGGCGAGGCGGGCGGGC
TCGGGCTGATCGGCGGCCTGGAGATCGTCGCCGACAAGGCCACCAAGCGGCAGTACGACCCGAAGAAGGGGGTGGCCGCC
CGCTGCGTCGCCTTCGCGCAGGGCGAGGGGCTGATCGTGCGCGCGCTCGCGGGGGACCGCGTCGCCGTCTGCCCGCCGCT
CATCATCGCGCCGGACGAGATCGACGCCCTGTTCGACCGGCTCGGCCGCGCGCTGGACCGCACCCGCGACTGGATCCGCG
CCGAGGGGCTGGAGGCGATGCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGTGCGGCAACGGCCGGACGCGGATCCTGTGGGCTACCTGCCGGGGCATCGGGTCGGTAGTGTGGCGGCGGTCACCCGC
GCACGAGGACCTTGCGAACC

Downstream 100 bases:

>100_bases
GGACCGCCCCACGCGTGGCGGCGCGGGCGCACATCCCGGCCGCGCTCGGCGCGCGCCGCCGTCACACCGCGTCGCGCGGC
CCGTCCGGCAGCGCGGCGGC

Product: class-III aminotransferase

Products: L-Glutamate; Succinic semialdehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MLSNLATRDVETLLHPYTNLSAHRQTGPLVLERGEGVHVYDTEGRSYIEGMSGLWCTALGYSNAELVEAARAQMARLPFT
HLFSGRSHDPGIELAETLKELMPVPTSKIFFTSSGSEANDTQVKLTWYYNNALGRPKKKKIIARQKGYHGVTVASASMTG
LAANHADWDLPLPGFLHVSPAHHYRGAEPGESEEAYSARLAAELEETILREGPDTVAAFIAEPVMGAGGAIVPPRGYFSA
IQAVLAKYDVRFIADEVICGFGRLGTWFGSEAVGARPDSISFAKALTSAYMPLGGVSIDEPLYEALIGQSEKIGTFGHGT
TYSGHPVAAAVALKAIEIYRRERVIEGAAEKAPQFQRRLAALAGHDIVGEAGGLGLIGGLEIVADKATKRQYDPKKGVAA
RCVAFAQGEGLIVRALAGDRVAVCPPLIIAPDEIDALFDRLGRALDRTRDWIRAEGLEAMPG

Sequences:

>Translated_462_residues
MLSNLATRDVETLLHPYTNLSAHRQTGPLVLERGEGVHVYDTEGRSYIEGMSGLWCTALGYSNAELVEAARAQMARLPFT
HLFSGRSHDPGIELAETLKELMPVPTSKIFFTSSGSEANDTQVKLTWYYNNALGRPKKKKIIARQKGYHGVTVASASMTG
LAANHADWDLPLPGFLHVSPAHHYRGAEPGESEEAYSARLAAELEETILREGPDTVAAFIAEPVMGAGGAIVPPRGYFSA
IQAVLAKYDVRFIADEVICGFGRLGTWFGSEAVGARPDSISFAKALTSAYMPLGGVSIDEPLYEALIGQSEKIGTFGHGT
TYSGHPVAAAVALKAIEIYRRERVIEGAAEKAPQFQRRLAALAGHDIVGEAGGLGLIGGLEIVADKATKRQYDPKKGVAA
RCVAFAQGEGLIVRALAGDRVAVCPPLIIAPDEIDALFDRLGRALDRTRDWIRAEGLEAMPG
>Mature_462_residues
MLSNLATRDVETLLHPYTNLSAHRQTGPLVLERGEGVHVYDTEGRSYIEGMSGLWCTALGYSNAELVEAARAQMARLPFT
HLFSGRSHDPGIELAETLKELMPVPTSKIFFTSSGSEANDTQVKLTWYYNNALGRPKKKKIIARQKGYHGVTVASASMTG
LAANHADWDLPLPGFLHVSPAHHYRGAEPGESEEAYSARLAAELEETILREGPDTVAAFIAEPVMGAGGAIVPPRGYFSA
IQAVLAKYDVRFIADEVICGFGRLGTWFGSEAVGARPDSISFAKALTSAYMPLGGVSIDEPLYEALIGQSEKIGTFGHGT
TYSGHPVAAAVALKAIEIYRRERVIEGAAEKAPQFQRRLAALAGHDIVGEAGGLGLIGGLEIVADKATKRQYDPKKGVAA
RCVAFAQGEGLIVRALAGDRVAVCPPLIIAPDEIDALFDRLGRALDRTRDWIRAEGLEAMPG

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=411, Percent_Identity=27.007299270073, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI13994255, Length=386, Percent_Identity=27.979274611399, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI37574042, Length=431, Percent_Identity=26.4501160092807, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI24119277, Length=447, Percent_Identity=26.3982102908277, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI226442705, Length=431, Percent_Identity=26.6821345707657, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI226442709, Length=398, Percent_Identity=27.3869346733668, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI284507298, Length=324, Percent_Identity=27.1604938271605, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1789016, Length=433, Percent_Identity=27.2517321016166, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1788044, Length=420, Percent_Identity=27.8571428571429, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI145693181, Length=351, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786991, Length=441, Percent_Identity=27.6643990929705, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789759, Length=419, Percent_Identity=28.1622911694511, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787560, Length=419, Percent_Identity=28.6396181384248, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=416, Percent_Identity=26.2019230769231, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI32564660, Length=458, Percent_Identity=26.8558951965066, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71992977, Length=425, Percent_Identity=24.7058823529412, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25144274, Length=225, Percent_Identity=31.5555555555556, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17541228, Length=459, Percent_Identity=21.7864923747277, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=429, Percent_Identity=25.8741258741259, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324386, Length=485, Percent_Identity=25.1546391752577, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324432, Length=428, Percent_Identity=25, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161085790, Length=460, Percent_Identity=27.8260869565217, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI28574759, Length=463, Percent_Identity=28.0777537796976, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21356575, Length=373, Percent_Identity=28.686327077748, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21357415, Length=420, Percent_Identity=25.7142857142857, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19 [C]

Molecular weight: Translated: 49550; Mature: 49550

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNLATRDVETLLHPYTNLSAHRQTGPLVLERGEGVHVYDTEGRSYIEGMSGLWCTALG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCHHHHHCCCCCEEEECC
YSNAELVEAARAQMARLPFTHLFSGRSHDPGIELAETLKELMPVPTSKIFFTSSGSEAND
CCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCC
TQVKLTWYYNNALGRPKKKKIIARQKGYHGVTVASASMTGLAANHADWDLPLPGFLHVSP
CEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCHHCCCCCCCCCCCCCCCEEECCC
AHHYRGAEPGESEEAYSARLAAELEETILREGPDTVAAFIAEPVMGAGGAIVPPRGYFSA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
IQAVLAKYDVRFIADEVICGFGRLGTWFGSEAVGARPDSISFAKALTSAYMPLGGVSIDE
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
PLYEALIGQSEKIGTFGHGTTYSGHPVAAAVALKAIEIYRRERVIEGAAEKAPQFQRRLA
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ALAGHDIVGEAGGLGLIGGLEIVADKATKRQYDPKKGVAARCVAFAQGEGLIVRALAGDR
HHHCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCE
VAVCPPLIIAPDEIDALFDRLGRALDRTRDWIRAEGLEAMPG
EEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSNLATRDVETLLHPYTNLSAHRQTGPLVLERGEGVHVYDTEGRSYIEGMSGLWCTALG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCHHHHHCCCCCEEEECC
YSNAELVEAARAQMARLPFTHLFSGRSHDPGIELAETLKELMPVPTSKIFFTSSGSEAND
CCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCC
TQVKLTWYYNNALGRPKKKKIIARQKGYHGVTVASASMTGLAANHADWDLPLPGFLHVSP
CEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCHHCCCCCCCCCCCCCCCEEECCC
AHHYRGAEPGESEEAYSARLAAELEETILREGPDTVAAFIAEPVMGAGGAIVPPRGYFSA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
IQAVLAKYDVRFIADEVICGFGRLGTWFGSEAVGARPDSISFAKALTSAYMPLGGVSIDE
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
PLYEALIGQSEKIGTFGHGTTYSGHPVAAAVALKAIEIYRRERVIEGAAEKAPQFQRRLA
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ALAGHDIVGEAGGLGLIGGLEIVADKATKRQYDPKKGVAARCVAFAQGEGLIVRALAGDR
HHHCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCE
VAVCPPLIIAPDEIDALFDRLGRALDRTRDWIRAEGLEAMPG
EEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-Aminobutanoate; 2-Oxoglutarate [C]

Specific reaction: 4-Aminobutanoate + 2-Oxoglutarate --> L-Glutamate + Succinic semialdehyde [C]

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]