Click here to switch to the map view.

The map label for this gene is splB [H]

Identifier: 170747263

GI number: 170747263

Start: 872468

End: 873598

Strand: Reverse

Name: splB [H]

Synonym: Mrad2831_0830

Alternate gene names: 170747263

Gene position: 873598-872468 (Counterclockwise)

Preceding gene: 170747265

Following gene: 170747262

Centisome position: 14.37

GC content: 75.33

Gene sequence:

>1131_bases
ATGCCGCCCTCGCCCGCCCCGGCCGTTGAGACCCGCGATCGCCCGGAGCGCCCGGGCCCGGCGCCCGATCGCCCGGCGCG
CCTGTGGCGCCCGCGCCGGGTGCTGGTGATGCCCGCTGCCCTCGAGCACGCTCACGGGCGAGCCATGCTGGCCCGCGCCG
AGGCGCTGGGCCTGCCGGTGGAGCGGCTGCCGTCGAACCGCCTGCCGAGCCCGCGCGAGGCGGATCCTCGCCGGGCCTAC
GCGGCCGCCAAGGCGACCCTCGCGCTCACGGTCGCGCCGCCGACCAAGCTGCGGCTGCAGCCGATCCCGCCCTCGGCCGA
CTGGCGCTTCGACCTCGCCGAGGGCTGCCCAGCCCACTGCCAGTACTGCTACCTCGCGGGCTCGCTGTCCGGGCCGCCGG
TGACCCGCGCCTACGCCAATCTGGCGGCGATTCTCGGCAGCCTCGCGGACTACGCGGGGCGCGGCGCGGTGACCTCGGCC
TCCGCCGAGCGGGCCCAGGAGGGGACCACCTTCGAGGCGTCCTGCTACACCGATCCCCTCGGCATCGAGCACCTCACCGG
CTCGCTCTCGGCGGCGATCCGGCATTTCGGCGCCTGGGACGCGCCGGTGCAGCTGCGCTTCACCACCAAGTTCGCCGCGG
TCGAGCCGCTCCTCGACCTGCCGCACAACCGGCGCACGCGCATCCGCTTCTCGGTCAATCCGCGGGCGGCCGCGCATTAC
GAGGGCGGCACCGATCCCCTCGACGCGCGGCTCGCCGCCATGGCGGCGGCGGCGCGGGCCGGCTATCCGGTCGGCCTCAC
CGTCGCGCCGATCCTGCCCGTCCCGGACTGGGAGGGCGCCTATGCCGGACTGATCCGCGCCGCCGCCGACGCGCTGGCCG
GCGTGCCCGATCTGGATCTCACCGCCGAACTCATCACCCACCGGTTCACGCCGGGCTCGAAGGCGGTGCTGCGCGGCTGG
TATCCCGGCTCGGACCTGCCCCTCGACGAGGCGGAGCGCGTCGAGAAGCGCACGAAGTTCGGCTCGGTGAAGCACGTCTT
CCCGAAGCCCCTGATGCAGGCCATGCGCGCCCATCTCACCGCGTGCCTCGCCCGCGACCTGCCGGCGGCGCGGATCCTGT
ACTGGACCTGA

Upstream 100 bases:

>100_bases
CATGTACGCTCTCCCTGCCTGCAGCCATGACGGTGCTTTAGCCGCACCGCGACCGGGCGACTAGGCCGGACCCGGTGCCG
GCACCGCGCGTTCGTCAGGC

Downstream 100 bases:

>100_bases
GCCCCACGGGCGCCATCTGGCCCCGATGATCGACGCGGACCCCCTGATCCTGACGCTCGCCTTCGACGAACCGACCTTCG
CGCGGTTCGACGGCGAGCGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MPPSPAPAVETRDRPERPGPAPDRPARLWRPRRVLVMPAALEHAHGRAMLARAEALGLPVERLPSNRLPSPREADPRRAY
AAAKATLALTVAPPTKLRLQPIPPSADWRFDLAEGCPAHCQYCYLAGSLSGPPVTRAYANLAAILGSLADYAGRGAVTSA
SAERAQEGTTFEASCYTDPLGIEHLTGSLSAAIRHFGAWDAPVQLRFTTKFAAVEPLLDLPHNRRTRIRFSVNPRAAAHY
EGGTDPLDARLAAMAAAARAGYPVGLTVAPILPVPDWEGAYAGLIRAAADALAGVPDLDLTAELITHRFTPGSKAVLRGW
YPGSDLPLDEAERVEKRTKFGSVKHVFPKPLMQAMRAHLTACLARDLPAARILYWT

Sequences:

>Translated_376_residues
MPPSPAPAVETRDRPERPGPAPDRPARLWRPRRVLVMPAALEHAHGRAMLARAEALGLPVERLPSNRLPSPREADPRRAY
AAAKATLALTVAPPTKLRLQPIPPSADWRFDLAEGCPAHCQYCYLAGSLSGPPVTRAYANLAAILGSLADYAGRGAVTSA
SAERAQEGTTFEASCYTDPLGIEHLTGSLSAAIRHFGAWDAPVQLRFTTKFAAVEPLLDLPHNRRTRIRFSVNPRAAAHY
EGGTDPLDARLAAMAAAARAGYPVGLTVAPILPVPDWEGAYAGLIRAAADALAGVPDLDLTAELITHRFTPGSKAVLRGW
YPGSDLPLDEAERVEKRTKFGSVKHVFPKPLMQAMRAHLTACLARDLPAARILYWT
>Mature_375_residues
PPSPAPAVETRDRPERPGPAPDRPARLWRPRRVLVMPAALEHAHGRAMLARAEALGLPVERLPSNRLPSPREADPRRAYA
AAKATLALTVAPPTKLRLQPIPPSADWRFDLAEGCPAHCQYCYLAGSLSGPPVTRAYANLAAILGSLADYAGRGAVTSAS
AERAQEGTTFEASCYTDPLGIEHLTGSLSAAIRHFGAWDAPVQLRFTTKFAAVEPLLDLPHNRRTRIRFSVNPRAAAHYE
GGTDPLDARLAAMAAAARAGYPVGLTVAPILPVPDWEGAYAGLIRAAADALAGVPDLDLTAELITHRFTPGSKAVLRGWY
PGSDLPLDEAERVEKRTKFGSVKHVFPKPLMQAMRAHLTACLARDLPAARILYWT

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 40483; Mature: 40352

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSPAPAVETRDRPERPGPAPDRPARLWRPRRVLVMPAALEHAHGRAMLARAEALGLPV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECHHHHHCCCHHHHHHHHHHCCCH
ERLPSNRLPSPREADPRRAYAAAKATLALTVAPPTKLRLQPIPPSADWRFDLAEGCPAHC
HHCCCCCCCCCCCCCHHHHHHHHEEEEEEEECCCCCEEEEECCCCCCCEEECCCCCCCCC
QYCYLAGSLSGPPVTRAYANLAAILGSLADYAGRGAVTSASAERAQEGTTFEASCYTDPL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCCCEEEEECCCCCC
GIEHLTGSLSAAIRHFGAWDAPVQLRFTTKFAAVEPLLDLPHNRRTRIRFSVNPRAAAHY
CHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCEECC
EGGTDPLDARLAAMAAAARAGYPVGLTVAPILPVPDWEGAYAGLIRAAADALAGVPDLDL
CCCCCHHHHHHHHHHHHHHCCCCCCEEEECEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
TAELITHRFTPGSKAVLRGWYPGSDLPLDEAERVEKRTKFGSVKHVFPKPLMQAMRAHLT
HHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHH
ACLARDLPAARILYWT
HHHHHCCCCCEEEEEC
>Mature Secondary Structure 
PPSPAPAVETRDRPERPGPAPDRPARLWRPRRVLVMPAALEHAHGRAMLARAEALGLPV
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECHHHHHCCCHHHHHHHHHHCCCH
ERLPSNRLPSPREADPRRAYAAAKATLALTVAPPTKLRLQPIPPSADWRFDLAEGCPAHC
HHCCCCCCCCCCCCCHHHHHHHHEEEEEEEECCCCCEEEEECCCCCCCEEECCCCCCCCC
QYCYLAGSLSGPPVTRAYANLAAILGSLADYAGRGAVTSASAERAQEGTTFEASCYTDPL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCCCEEEEECCCCCC
GIEHLTGSLSAAIRHFGAWDAPVQLRFTTKFAAVEPLLDLPHNRRTRIRFSVNPRAAAHY
CHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCEECC
EGGTDPLDARLAAMAAAARAGYPVGLTVAPILPVPDWEGAYAGLIRAAADALAGVPDLDL
CCCCCHHHHHHHHHHHHHHCCCCCCEEEECEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
TAELITHRFTPGSKAVLRGWYPGSDLPLDEAERVEKRTKFGSVKHVFPKPLMQAMRAHLT
HHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHH
ACLARDLPAARILYWT
HHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]