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The map label for this gene is ruvB [H]

Identifier: 170747185

GI number: 170747185

Start: 795357

End: 796412

Strand: Reverse

Name: ruvB [H]

Synonym: Mrad2831_0751

Alternate gene names: 170747185

Gene position: 796412-795357 (Counterclockwise)

Preceding gene: 170747188

Following gene: 170747184

Centisome position: 13.1

GC content: 70.45

Gene sequence:

>1056_bases
ATGAGCAAGCCGAAGCCTCCGACCAACGCGCTCCTGACGCCGGAGAAGCGCACCGACGACGTGGATCAGACGATCCGCCC
TCTGAGCCTGTCGGAGTTCATCGGTCAGCGTGCGGCACGTGCGAACATGCAGGTGTTCATCGAGGCTGCGAAGAAGACCG
GTCAGGCGCTCGACCACGTGCTGTTCGTCGGTCCGCCGGGGCTGGGCAAGACGACGCTGGCCCAGATCGTGGCGCGCGAG
CTGGGCGTGAACTTCCGCTCGACCTCCGGGCCGGTGATCGCCAAGGCCGGCGACCTGGCCGCGCAGCTCACCAACCTGGA
GGAGCGCGACGTCCTGTTCATCGACGAGATCCACCGGCTCAACCCGGCGGTCGAGGAGATCCTCTACCCGGCCATGGAAG
ACTACCAGCTCGACCTGATCATCGGCGAGGGCCCGGCGGCGCGCTCGGTCAAGATCGAGCTGCCGAAGTTCACCCTGGTC
GGCGCCACGACGCGGGCGGGGCTGCTGACGACGCCGCTGCGCGACCGGTTCGGGATCCCGATCCGGCTGGAGTTCTACGA
GATCGACGAGCTGGAGCAGATCGTGGCGCGCGGCGCGCGGGTCCTGGGCTTGGGCATGTCGGCGGAGGGCGCCAACGAGA
TCGCCCGGCGGGCCCGGGGCACGCCGCGGATCGCGGGGCGGCTGTTGCGGCGGGTGCGGGATTTCGCGGTCGTGGCGGAG
GCCGAGACGGTGACGCGGGCGATCGCCGACCGGGCGCTGCAGCTCCTGGACGTGGACGGGGCGGGTCTGGACGTGATGGA
CCGCAAGTACCTGAGCCTGATCGCGCGCGCGTTCGGGGGCGGCCCGGTGGGGATCGAGACCATCGGCGCGGCCTTGTCGG
AGCCGCGCGACGCCATCGAGGACATCATCGAGCCCTACCTGATCCAGCGCGGCTTCGTGCAGCGCACGCCCCGCGGCCGC
GTCCTCACACGACACGCGTACCGGCACATGCAGATCCCGGAACCGACGCGCGAGACCCCCGGGCAGTTCGGCTTCTTCGG
CGACGAGGAGCCGTGA

Upstream 100 bases:

>100_bases
TTCACGGGTAAGGAAGAACAAAACCGGACGCGCCGGTGCCCAGCCGTCCTGCGTGGCCGCCCATCGCGGGCCGCGCGGCA
CGGTCCACGAAGCAGGGTTC

Downstream 100 bases:

>100_bases
GGCGGCCCCGACCGCTCTATGCACTGAGTTCGGACCAGCGTTTCCCGCGGCACATGCGCTCCGCGCGCCCCGATCCGCGC
CCCTGCCGGCGCGATCGCGC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSKPKPPTNALLTPEKRTDDVDQTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVARE
LGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLV
GATTRAGLLTTPLRDRFGIPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVVAE
AETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIEDIIEPYLIQRGFVQRTPRGR
VLTRHAYRHMQIPEPTRETPGQFGFFGDEEP

Sequences:

>Translated_351_residues
MSKPKPPTNALLTPEKRTDDVDQTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVARE
LGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLV
GATTRAGLLTTPLRDRFGIPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVVAE
AETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIEDIIEPYLIQRGFVQRTPRGR
VLTRHAYRHMQIPEPTRETPGQFGFFGDEEP
>Mature_350_residues
SKPKPPTNALLTPEKRTDDVDQTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVAREL
GVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVG
ATTRAGLLTTPLRDRFGIPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVVAEA
ETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIEDIIEPYLIQRGFVQRTPRGRV
LTRHAYRHMQIPEPTRETPGQFGFFGDEEP

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=317, Percent_Identity=64.6687697160883, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787119, Length=146, Percent_Identity=32.1917808219178, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 38502; Mature: 38371

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPKPPTNALLTPEKRTDDVDQTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHV
CCCCCCCCCCEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCE
LFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHCCCCCCEEHHHHHHHC
NPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVGATTRAGLLTTPLRDRFGIP
CHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEECCHHHHCCCC
IRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVVAE
EEEEEECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIE
HHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
DIIEPYLIQRGFVQRTPRGRVLTRHAYRHMQIPEPTRETPGQFGFFGDEEP
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKPKPPTNALLTPEKRTDDVDQTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHV
CCCCCCCCCEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCE
LFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHCCCCCCEEHHHHHHHC
NPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVGATTRAGLLTTPLRDRFGIP
CHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEECCHHHHCCCC
IRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVVAE
EEEEEECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIE
HHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
DIIEPYLIQRGFVQRTPRGRVLTRHAYRHMQIPEPTRETPGQFGFFGDEEP
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]