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The map label for this gene is paaF [C]

Identifier: 170747164

GI number: 170747164

Start: 770636

End: 771448

Strand: Reverse

Name: paaF [C]

Synonym: Mrad2831_0730

Alternate gene names: 170747164

Gene position: 771448-770636 (Counterclockwise)

Preceding gene: 170747165

Following gene: 170747163

Centisome position: 12.69

GC content: 73.92

Gene sequence:

>813_bases
ATGACCGCAGCCCTTGCCACGGCCCCCGCCGCCACCCCGGAGGCGCCTGTGCTGCTGCGCAGCGACGCCGACGGCGTCGC
GACCCTGACGCTGAACCGGCCGCAGGCCCGCAACGCCCTGTCGATGGCCCTGATGGGCGCGCTCCAGGCGCAGCTCGACG
CGATCCGCGCGGATCCGTCCGTGCGGGTCGTGATCCTGCAGGGGGCAGGCCCCGCCTTCTGCGCCGGGCACGACCTCAAG
GAGATGCGCGCCGACCCGTCCCGGGAGGCCACCGAGGCGGTGTTCCGCACCTGCGCCCGGCTGATGCTGAGCCTCACGCG
CCTGCCGCAACCGGTGATCGCCCGGGTGCACGGGATCGCCACCGCCGCCGGGTGCCAGCTCGTCGCCACCTGCGACCTCG
CCGTCTGCGCCGAGACCGCCCGCTTCGCCACGCCGGGGGTGCAGATCGGCCTGTTCTGCTCGACCCCGATGGTCGCCCTG
TCGCGGGCCGTGTCGCGCAAGGCCGCCCTGGAGATGCTGCTGGTCGGCGACCCGATCGACGCCGCGGAGGCTCTGCGGAT
CGGACTCGTGAACCGCGCCGTGCCGGAGGCGGATCTCGACGCGGCGGTGAGCGCCCTCGCGGCCCGGATCGCCGGCAAGG
CCCGGCGCGTCCTGGCGATCGGCAAGGAGGCGTTCGGCCGGCAGATCGAGATGAGCCTGGAGGACGCCTACGGTTACACG
GCGGAGGTCATGACCCGCAACATGATGATGGCCGACGCGCAGGAGGGGATCGACGCCTTCCTGGGCAAGCGACCGCCCCG
CTGGGAGCCGTGA

Upstream 100 bases:

>100_bases
GGCCGGGCCGCGGCGCCGTCGCGAACCGGAAAACCGCGTGCTACCGTCCGGCGACGCGACCGGCCCCGCCGCAGGGAGGC
CGGCGCCGGGAGACGCCGCC

Downstream 100 bases:

>100_bases
GCCGGCCTCCTGGCGCGATACGACGCAGGCGGTTCTGCGCGGCGCGCGTAGGTGATGGCGCCGCCCTGCTCTAGGATGAG
GGCCTGTCCGATCCTGCCGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTAALATAPAATPEAPVLLRSDADGVATLTLNRPQARNALSMALMGALQAQLDAIRADPSVRVVILQGAGPAFCAGHDLK
EMRADPSREATEAVFRTCARLMLSLTRLPQPVIARVHGIATAAGCQLVATCDLAVCAETARFATPGVQIGLFCSTPMVAL
SRAVSRKAALEMLLVGDPIDAAEALRIGLVNRAVPEADLDAAVSALAARIAGKARRVLAIGKEAFGRQIEMSLEDAYGYT
AEVMTRNMMMADAQEGIDAFLGKRPPRWEP

Sequences:

>Translated_270_residues
MTAALATAPAATPEAPVLLRSDADGVATLTLNRPQARNALSMALMGALQAQLDAIRADPSVRVVILQGAGPAFCAGHDLK
EMRADPSREATEAVFRTCARLMLSLTRLPQPVIARVHGIATAAGCQLVATCDLAVCAETARFATPGVQIGLFCSTPMVAL
SRAVSRKAALEMLLVGDPIDAAEALRIGLVNRAVPEADLDAAVSALAARIAGKARRVLAIGKEAFGRQIEMSLEDAYGYT
AEVMTRNMMMADAQEGIDAFLGKRPPRWEP
>Mature_269_residues
TAALATAPAATPEAPVLLRSDADGVATLTLNRPQARNALSMALMGALQAQLDAIRADPSVRVVILQGAGPAFCAGHDLKE
MRADPSREATEAVFRTCARLMLSLTRLPQPVIARVHGIATAAGCQLVATCDLAVCAETARFATPGVQIGLFCSTPMVALS
RAVSRKAALEMLLVGDPIDAAEALRIGLVNRAVPEADLDAAVSALAARIAGKARRVLAIGKEAFGRQIEMSLEDAYGYTA
EVMTRNMMMADAQEGIDAFLGKRPPRWEP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=48.1781376518219, Blast_Score=242, Evalue=2e-64,
Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=33.0708661417323, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI70995211, Length=208, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=23.921568627451, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=23.921568627451, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=23.921568627451, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594469, Length=142, Percent_Identity=33.0985915492958, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI37594471, Length=142, Percent_Identity=33.0985915492958, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757968, Length=246, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI49355787, Length=246, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757966, Length=246, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538581, Length=246, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI25453481, Length=246, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI51538573, Length=246, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI62530384, Length=202, Percent_Identity=30.6930693069307, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=28.8, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=253, Percent_Identity=26.8774703557312, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=253, Percent_Identity=26.8774703557312, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=253, Percent_Identity=26.8774703557312, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=25.6521739130435, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=25.6521739130435, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI295842266, Length=199, Percent_Identity=30.1507537688442, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=34.251968503937, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=34.2629482071713, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=31.1284046692607, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=30.9236947791165, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=27.4131274131274, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788682, Length=238, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=204, Percent_Identity=26.9607843137255, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=43.3198380566802, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=29.811320754717, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=33.2, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=32.3232323232323, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=25.0965250965251, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=288, Percent_Identity=27.0833333333333, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=27.5862068965517, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=188, Percent_Identity=27.1276595744681, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=24.6445497630332, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=25.6281407035176, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=39.9209486166008, Blast_Score=213, Evalue=9e-56,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=30.241935483871, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=30.241935483871, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=30.2325581395349, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357171, Length=273, Percent_Identity=28.9377289377289, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24650670, Length=259, Percent_Identity=29.7297297297297, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654903, Length=180, Percent_Identity=28.3333333333333, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=228, Percent_Identity=28.9473684210526, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583165, Length=176, Percent_Identity=26.7045454545455, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28373; Mature: 28242

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALATAPAATPEAPVLLRSDADGVATLTLNRPQARNALSMALMGALQAQLDAIRADPS
CCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VRVVILQGAGPAFCAGHDLKEMRADPSREATEAVFRTCARLMLSLTRLPQPVIARVHGIA
EEEEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TAAGCQLVATCDLAVCAETARFATPGVQIGLFCSTPMVALSRAVSRKAALEMLLVGDPID
HHHCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHEEEEECCCCC
AAEALRIGLVNRAVPEADLDAAVSALAARIAGKARRVLAIGKEAFGRQIEMSLEDAYGYT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEHHHHCCHH
AEVMTRNMMMADAQEGIDAFLGKRPPRWEP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAALATAPAATPEAPVLLRSDADGVATLTLNRPQARNALSMALMGALQAQLDAIRADPS
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VRVVILQGAGPAFCAGHDLKEMRADPSREATEAVFRTCARLMLSLTRLPQPVIARVHGIA
EEEEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TAAGCQLVATCDLAVCAETARFATPGVQIGLFCSTPMVALSRAVSRKAALEMLLVGDPID
HHHCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHEEEEECCCCC
AAEALRIGLVNRAVPEADLDAAVSALAARIAGKARRVLAIGKEAFGRQIEMSLEDAYGYT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEHHHHCCHH
AEVMTRNMMMADAQEGIDAFLGKRPPRWEP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA