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The map label for this gene is proA

Identifier: 170746553

GI number: 170746553

Start: 108401

End: 109699

Strand: Reverse

Name: proA

Synonym: Mrad2831_0103

Alternate gene names: 170746553

Gene position: 109699-108401 (Counterclockwise)

Preceding gene: 170746554

Following gene: 170746552

Centisome position: 1.8

GC content: 73.36

Gene sequence:

>1299_bases
GTGCCCGTCCTGAACCTGAGATCGGACTTCGCCGAGGCCGATGCCCTCCCGGAGCAGATGGGGGCGATCGGCCGTCGCGC
CCGGGCGGCGGCGCGGCGCATGGCGCTGGCCTCGGCGCGGACCAAGGACGCGGCGCTCAAGCTGATCGCCGAGCGGATCC
GCGCGAGCCGCGACGAGATCCTGAGCGAGAACGCGCGGGACGTGGCCGCGGCCCGGTGCGCGGGTCAGACCGCCGCGCTG
ATCGACCGGCTCACCCTCGACCCTGGGCGCCTCGCGGCCATTGCGGACGCCGTCGAGAAGGTCGGGTCGCTGGCCGACCC
GGTCGGTCGCCAGCTCGCCGCGATCGAGCGGCCGAACGGCCTGCTGATCGAGCGCATCGCGGTGCCGCTCGGGGTCGTCG
GCGTGATCTTCGAGGCGCGGCCCAACGTGACGGCGGATGCCGGCGCGCTCTGCCTCAAGGCCGGCAACGCGGCGATCCTT
CGCGCCGGTTCGGACAGCCATCGCAGCGCCATGGCGATCGCGAGGGCCATGAGCCGCGGGCTCGCCGATGCCGGCCTGCC
GGAGGACGCGATCCAGCTGGTGCCGACCCGCGACCGCGCCGCGGTCGGGCTGATGCTCGCCGGCCTCGACGGCTGCGTGG
ACGTGATCGTCCCGCGCGGCGGCCGCGGCCTCGTGGAGCGCGTCCAGGCCGAGGCGCGCGTTCCGGTCTTCGCCCATCTC
GACGGCATCAACCACGTCTACGTCGCCGCGGCGGCCGACCTCGACATGGCCCGCACCGTGCTTCTCAACAGCAAGATGCG
CCGGACCAGCGTCTGCGGCGCCGCCGAGACCCTGCTGGTCGACCGTGCCTGCGCGGCGACGCACCTCGCGCCGCTGGTCC
AGGCCCTGCTCGACGCCGGCTGCGCGGTCCGGGGCGATGCCGCGACGCGCGCGGTCGATCCGCGCGTGACGGCCGCTTCC
GAGGAGGATTGGCACACCGAGTACCTCGACGCGATCATCGCGGTGCGGGTGGTGGACGGGATCGAGGCCGCCATCGAGCA
CATCGAGACCTACGGATCGCATCACACGGACGCGATCATCACCGAGAACGACGCCGAGGCGACCCGTTTCCTGGCCGAAG
TCGACTCGGCGATCGTCACGCACAACGCCTCCACGCAGTTCGCCGACGGCGGCGAGTTCGGCTTCGGGGCCGAGATCGGC
ATCGCCACGGGGCGGATGCACGCGCGCGGGCCGGTGGGCGTCGAGCAGCTCACCACGTTCAAGTACCGGGTCCACGGCAC
CGGCCAGATTCGCCCGTGA

Upstream 100 bases:

>100_bases
CCGGCCCGGCACTCTTGTCGGGACGGCGCTCGATGTGCAAATACGGCGCTCTCCCCCGTCCGGGTCGCGCGTGCGGCCGT
CGACAACCGAGGACGCCAGC

Downstream 100 bases:

>100_bases
TCCGACCTGAAGGTCCGGCGTGCGGCTGACCCTGCCGCCGAGCGCCCCCGGCATGCGGATCGGCCTCTACGGCGGATCCT
TCAATCCGGCCCATCTGGGC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPVLNLRSDFAEADALPEQMGAIGRRARAAARRMALASARTKDAALKLIAERIRASRDEILSENARDVAAARCAGQTAAL
IDRLTLDPGRLAAIADAVEKVGSLADPVGRQLAAIERPNGLLIERIAVPLGVVGVIFEARPNVTADAGALCLKAGNAAIL
RAGSDSHRSAMAIARAMSRGLADAGLPEDAIQLVPTRDRAAVGLMLAGLDGCVDVIVPRGGRGLVERVQAEARVPVFAHL
DGINHVYVAAAADLDMARTVLLNSKMRRTSVCGAAETLLVDRACAATHLAPLVQALLDAGCAVRGDAATRAVDPRVTAAS
EEDWHTEYLDAIIAVRVVDGIEAAIEHIETYGSHHTDAIITENDAEATRFLAEVDSAIVTHNASTQFADGGEFGFGAEIG
IATGRMHARGPVGVEQLTTFKYRVHGTGQIRP

Sequences:

>Translated_432_residues
MPVLNLRSDFAEADALPEQMGAIGRRARAAARRMALASARTKDAALKLIAERIRASRDEILSENARDVAAARCAGQTAAL
IDRLTLDPGRLAAIADAVEKVGSLADPVGRQLAAIERPNGLLIERIAVPLGVVGVIFEARPNVTADAGALCLKAGNAAIL
RAGSDSHRSAMAIARAMSRGLADAGLPEDAIQLVPTRDRAAVGLMLAGLDGCVDVIVPRGGRGLVERVQAEARVPVFAHL
DGINHVYVAAAADLDMARTVLLNSKMRRTSVCGAAETLLVDRACAATHLAPLVQALLDAGCAVRGDAATRAVDPRVTAAS
EEDWHTEYLDAIIAVRVVDGIEAAIEHIETYGSHHTDAIITENDAEATRFLAEVDSAIVTHNASTQFADGGEFGFGAEIG
IATGRMHARGPVGVEQLTTFKYRVHGTGQIRP
>Mature_431_residues
PVLNLRSDFAEADALPEQMGAIGRRARAAARRMALASARTKDAALKLIAERIRASRDEILSENARDVAAARCAGQTAALI
DRLTLDPGRLAAIADAVEKVGSLADPVGRQLAAIERPNGLLIERIAVPLGVVGVIFEARPNVTADAGALCLKAGNAAILR
AGSDSHRSAMAIARAMSRGLADAGLPEDAIQLVPTRDRAAVGLMLAGLDGCVDVIVPRGGRGLVERVQAEARVPVFAHLD
GINHVYVAAAADLDMARTVLLNSKMRRTSVCGAAETLLVDRACAATHLAPLVQALLDAGCAVRGDAATRAVDPRVTAASE
EDWHTEYLDAIIAVRVVDGIEAAIEHIETYGSHHTDAIITENDAEATRFLAEVDSAIVTHNASTQFADGGEFGFGAEIGI
ATGRMHARGPVGVEQLTTFKYRVHGTGQIRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=36.8038740920097, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=36.8038740920097, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786438, Length=418, Percent_Identity=41.866028708134, Blast_Score=308, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17569967, Length=424, Percent_Identity=35.6132075471698, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17569969, Length=424, Percent_Identity=35.6132075471698, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=35.1981351981352, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI21357643, Length=423, Percent_Identity=34.2789598108747, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_METRJ (B1M695)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001752813.1
- ProteinModelPortal:   B1M695
- SMR:   B1M695
- GeneID:   6136403
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0103
- HOGENOM:   HBG318080
- OMA:   GICGAME
- ProtClustDB:   PRK00197
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45312; Mature: 45181

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLNLRSDFAEADALPEQMGAIGRRARAAARRMALASARTKDAALKLIAERIRASRDEI
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LSENARDVAAARCAGQTAALIDRLTLDPGRLAAIADAVEKVGSLADPVGRQLAAIERPNG
HHCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LLIERIAVPLGVVGVIFEARPNVTADAGALCLKAGNAAILRAGSDSHRSAMAIARAMSRG
HHHHHHHHHHHHHHHHEECCCCCCCCCCHHEEECCCEEEEECCCCCHHHHHHHHHHHHHC
LADAGLPEDAIQLVPTRDRAAVGLMLAGLDGCVDVIVPRGGRGLVERVQAEARVPVFAHL
CCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEE
DGINHVYVAAAADLDMARTVLLNSKMRRTSVCGAAETLLVDRACAATHLAPLVQALLDAG
CCCCEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CAVRGDAATRAVDPRVTAASEEDWHTEYLDAIIAVRVVDGIEAAIEHIETYGSHHTDAII
CEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TENDAEATRFLAEVDSAIVTHNASTQFADGGEFGFGAEIGIATGRMHARGPVGVEQLTTF
ECCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHEEE
KYRVHGTGQIRP
EEEEECCCCCCC
>Mature Secondary Structure 
PVLNLRSDFAEADALPEQMGAIGRRARAAARRMALASARTKDAALKLIAERIRASRDEI
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LSENARDVAAARCAGQTAALIDRLTLDPGRLAAIADAVEKVGSLADPVGRQLAAIERPNG
HHCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LLIERIAVPLGVVGVIFEARPNVTADAGALCLKAGNAAILRAGSDSHRSAMAIARAMSRG
HHHHHHHHHHHHHHHHEECCCCCCCCCCHHEEECCCEEEEECCCCCHHHHHHHHHHHHHC
LADAGLPEDAIQLVPTRDRAAVGLMLAGLDGCVDVIVPRGGRGLVERVQAEARVPVFAHL
CCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEE
DGINHVYVAAAADLDMARTVLLNSKMRRTSVCGAAETLLVDRACAATHLAPLVQALLDAG
CCCCEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CAVRGDAATRAVDPRVTAASEEDWHTEYLDAIIAVRVVDGIEAAIEHIETYGSHHTDAII
CEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TENDAEATRFLAEVDSAIVTHNASTQFADGGEFGFGAEIGIATGRMHARGPVGVEQLTTF
ECCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHEEE
KYRVHGTGQIRP
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA