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The map label for this gene is metK

Identifier: 170762349

GI number: 170762349

Start: 474876

End: 476030

Strand: Direct

Name: metK

Synonym: UPA3_0429

Alternate gene names: 170762349

Gene position: 474876-476030 (Clockwise)

Preceding gene: 170762421

Following gene: 170762417

Centisome position: 63.18

GC content: 29.78

Gene sequence:

>1155_bases
ATGGTAGAAAGAAAAGAATTTAGTATGAAATATAAAAAAATTATAACGAGCGAATCTGTTGGTGCAGGCCATCCAGATAA
AATATGTGATCAGATTAGTGATGCAATTTTAGATGAATGCTTAGCACAAGATAAAAATTCACGTGTAGCTTGTGAAGTTT
TAGCTTGTAATCGTTTAATTGTTATTGCTGGGGAAATTACCACGCATGCATATGTTGATGTAGTAAAAACCGCTTGAGAA
ATTATTAAACCTTTAGGCTATAATGAAAATGATTTTACGATTATTTCTAATGTTAATAAACAATCAGTAGATATAGCGCA
AAGTGTTAATAAAGCAAATAAAGATTTAATTGGAGCTGGTGACCAAGGAATTGTTTTTGGCTATGCTTGTGATGAAACAC
CTCAATATATGCCATTAACAACAGTGTTAGCTCATGAATTACTAAAAGAAATCGAAAAACAAAGACGTAATAAAGAATTT
GTTAAAATTCAAGCTGATATGAAATCACAAGTAAGTATTGATTATTCTAATTGTACGCCTGTTATTGAGACAATGTTGGT
AAGTATTCAACATGATGAAGATTATAACATTGAATATTTTAATAAAAAAGTTAGCACAATTATGGATCAAATTGCTATAA
AATATGGTTTAAATACCGATTTTAAAAAAATTATTAATTCCTCAGGGAGATTTGTAATTGGTGGTCCAATTGGTGACACT
GGTTTAACTGGAAGAAAAATTATCGTAGATACTTATGGAGGAATAGGTCATCATGGTGGTGGTGCTTTTAGTGGCAAAGA
CCCTACTAAAGTTGATCGTAGTGCTTCATACTTTGCACGTTGAATAGCTAAAAATGTTGTTGCAGCTAAGCTTGCTAAAC
AGTGTGAAATTCAATTAGCTTTTGCAATTGGAGAACCACAACCTGTGGCAATGTTTGTTAATACATTCAAGACTAATTTG
GTTGATGAAAAGAAAATTTTTGAAGCAATTAATAAAAGTTTTAATTTTGATATCAAAACGTTTATTAATGATTTAGAATT
GTGAACAACTAAATATTTACCAGTAGCAACTTATGGTCATTTCGGTCGTGATGATTTAAACTTAAGTTGAGAAAAATTAA
ATAAGGTTCAAGATTTAATAAAATATAGTAAATAA

Upstream 100 bases:

>100_bases
ATATTCCAGAAATGATTTATACAGGTGATAATGCTGCTATGATTGGGGCTTATGCTTATGCTTTAATTAAAAATCATAAG
AAATCAATATTAATAAAATA

Downstream 100 bases:

>100_bases
ATTTAAAAAACGATACGCACAATAAAGGGCATATCGTTTTTTTAATAAATAATATAATGGTGCTCAAGAAGGGACTCGAA
CCCTTATGATTTCTCGGTTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTAWE
IIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEF
VKIQADMKSQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDT
GLTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFARWIAKNVVAAKLAKQCEIQLAFAIGEPQPVAMFVNTFKTNL
VDEKKIFEAINKSFNFDIKTFINDLELWTTKYLPVATYGHFGRDDLNLSWEKLNKVQDLIKYSK

Sequences:

>Translated_384_residues
MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTA*E
IIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEF
VKIQADMKSQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDT
GLTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFAR*IAKNVVAAKLAKQCEIQLAFAIGEPQPVAMFVNTFKTNL
VDEKKIFEAINKSFNFDIKTFINDLEL*TTKYLPVATYGHFGRDDLNLS*EKLNKVQDLIKYSK
>Mature_384_residues
MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTA*E
IIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEF
VKIQADMKSQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDT
GLTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFAR*IAKNVVAAKLAKQCEIQLAFAIGEPQPVAMFVNTFKTNL
VDEKKIFEAINKSFNFDIKTFINDLEL*TTKYLPVATYGHFGRDDLNLS*EKLNKVQDLIKYSK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=366, Percent_Identity=45.9016393442623, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI4557737, Length=368, Percent_Identity=44.8369565217391, Blast_Score=275, Evalue=6e-74,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=44.9468085106383, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=43.9897698209719, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=43.3510638297872, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17538498, Length=379, Percent_Identity=43.2717678100264, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17551082, Length=377, Percent_Identity=41.9098143236074, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI32565905, Length=368, Percent_Identity=40.4891304347826, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI25145635, Length=342, Percent_Identity=41.812865497076, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=41.2121212121212, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI32565903, Length=293, Percent_Identity=41.6382252559727, Blast_Score=207, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=50.6172839506173, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323209, Length=363, Percent_Identity=46.2809917355372, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=44.7089947089947, Blast_Score=280, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=45.0402144772118, Blast_Score=270, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=45.0402144772118, Blast_Score=270, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=44.0443213296399, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=45.7627118644068, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42179; Mature: 42179

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLI
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEE
VIAGEITTHAYVDVVKTAEIIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGD
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
QGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMKSQVSIDYSNCTPV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHH
IETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTG
HHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCC
LTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFARIAKNVVAAKLAKQCEIQLAFA
CCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
IGEPQPVAMFVNTFKTNLVDEKKIFEAINKSFNFDIKTFINDLELTTKYLPVATYGHFGR
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCC
DDLNLSEKLNKVQDLIKYSK
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLI
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEE
VIAGEITTHAYVDVVKTAEIIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGD
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
QGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMKSQVSIDYSNCTPV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHH
IETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTG
HHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCC
LTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFARIAKNVVAAKLAKQCEIQLAFA
CCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
IGEPQPVAMFVNTFKTNLVDEKKIFEAINKSFNFDIKTFINDLELTTKYLPVATYGHFGR
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCC
DDLNLSEKLNKVQDLIKYSK
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]