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The map label for this gene is cmoA [H]

Identifier: 170684168

GI number: 170684168

Start: 1306785

End: 1307528

Strand: Reverse

Name: cmoA [H]

Synonym: EcSMS35_1316

Alternate gene names: 170684168

Gene position: 1307528-1306785 (Counterclockwise)

Preceding gene: 170680079

Following gene: 170683171

Centisome position: 25.8

GC content: 49.87

Gene sequence:

>744_bases
ATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGT
CTTCCCGGATATGATCCAGCGTTCCGTTCCCGGCTATTCCAATATTATTTCCATGATTGGTATGTTAGCCGAGCGCTTCG
TTCAACCTGGTACGCAGGTTTACGATCTGGGTTGTTCTCTGGGCGCGGCGACGCTCTCGGTGCGTCGCAACATTCATCAT
GATAATTGCAAAATTATTGCCATCGATAACTCCCCGGCGATGATTGAACGCTGCCGTCGTCATATTGACGCCTATAAAGC
TCCTACGCCAGTAGACGTTATTGAAGGTGATATTCGCGATATCGCCATTGAAAATGCATCGATGGTGGTGCTGAATTTTA
CCCTGCAATTCCTGGAACCTTCCGAGCGCCAGGCGTTACTGGATAAAATTTATCAAGGACTGAACCCGGGCGGCGCTCTG
GTGCTTTCGGAAAAATTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGCTGTTCAACATGCACCACGACTTTAAACGTGC
CAACGGTTACAGCGAACTGGAGATCAGCCAGAAACGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCC
ATAAAGCACGCCTGCATAAAGCCGGTTTTGAGCATAGCGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCA
TTAAAAGCAGAGGACGCTGCATGA

Upstream 100 bases:

>100_bases
CTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCT
TTTTCCCGGATTTTTACGTT

Downstream 100 bases:

>100_bases
TCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTGGAAACGCTGCCCGCGCAGATTGCT
AACTGGCAGCGCGAACAGCA

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHH
DNCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVA
LKAEDAA

Sequences:

>Translated_247_residues
MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHH
DNCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVA
LKAEDAA
>Mature_246_residues
SHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHD
NCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGALV
LSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVAL
KAEDAA

Specific function: Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoA family [H]

Homologues:

Organism=Escherichia coli, GI1788177, Length=247, Percent_Identity=99.5951417004049, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR005271 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: NA

Molecular weight: Translated: 27791; Mature: 27660

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQV
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
YDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRD
EECCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHH
IAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGALVLSEKFSFEDAKVGELLFNM
EEECCCCEEEEEEEHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
HHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
LKAEDAA
EECCCCC
>Mature Secondary Structure 
SHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQV
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
YDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRD
EECCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHH
IAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGALVLSEKFSFEDAKVGELLFNM
EEECCCCEEEEEEEHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
HHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
LKAEDAA
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA