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The map label for this gene is ada [H]

Identifier: 170684032

GI number: 170684032

Start: 2393061

End: 2394125

Strand: Reverse

Name: ada [H]

Synonym: EcSMS35_2362

Alternate gene names: 170684032

Gene position: 2394125-2393061 (Counterclockwise)

Preceding gene: 170681131

Following gene: 170682238

Centisome position: 47.24

GC content: 57.18

Gene sequence:

>1065_bases
ATGAAAAACGCCACATGCTTAACAGACGATCAACGCTGGCAATCTGTCTTAGCCCGCGACCCGAATGCCGACGGCGAATT
CGTTTTCGCCGTGCGCACCACAGGTATCTTTTGCCGTCCGTCTTGCCGCGCCAGACATGCCTTGCGGGAAAATGTCTCCT
TCTACGCAAATGCCAGCGAGGCACTCGCCGCCGGATTTCGCCCCTGCAAACGTTGTCAGCCAGACAAAGCCAATGCCCAG
CAACATCGCCTTGATAAAATTACCCACGCCTGCCGACTTCTGGAACAGGAAACGCCTGTAACGCTGGAAGCCTTAGCCGA
CCATGTGGCGATGAGTCCGTTCCATCTGCATCGGTTGTTTAAAGCGACTACCGGAATGACGCCTAAAGCCTGGCAACAGG
CCTGGCGCGCTCGCCGTTTGCGCGAATCGCTGGCGAAAGGGGAGAGCGTGACGACGTCTATTCTTAACGCCGGATTCCCC
GACAGCAGCAGTTACTACCGCAAAGCTGACGAAACGCTGGGCATGACGGCTAAACAATTCCGTCACGGTGGCGAAAATCT
GGCGGTGCGTTACGCGCTGGCTGATTGTGAGCTGGGCCGTTGCCTGGTGGCAGAAAGTGAGCGGGGGATTTGCGCGATAT
TACTGGGCGATGATGACGCGACACTAATCAGCGAGTTACAGCAGATGTTTCCCGCTGCCGACAGCGCGCCTGCCGATCTG
ACGTTTCAGCAACATGTGCGTGAAGTGATCGCCAGCCTCAATCAACGCGATACGCCGCTGACGTTACCGCTGGACATTCG
CGGCACTGCTTTTCAGCAACAAGTCTGGCAGGCACTGCGCACGATACCTTGCGGTGAAACCGTCAGTTATCAGCAACTGG
CTAATGCCATCGGCAAACCGAAAGCGGTACGGGCTGTTGCCAGCGCCTGTGCCGCCAACAAGCTAGCTATCGTTATACCT
TGTCATCGGGTGGTCCGTGGTGATGGCACACTTTCCGGTTACCGCTGGGGCGTGTCGCGCAAAGCGCAACTGCTGCGCCG
CGAAGCTGAGAATGAGGAGAGGTAA

Upstream 100 bases:

>100_bases
AGCTTCCTTATCAGCGAAAAAAATTAAAGCGCAAGATTGTTGGTTTTTGCGCGATGGTGACCGGGCAGACTAAAGGCTAT
CCTTAACCAGGGAGCTGATT

Downstream 100 bases:

>100_bases
TGTTGGATCTGTTTGCCGATGCTGAACCGTGGCAAGAGCCACTGGCGGCTGGTGCGGTAATTTTACGGCGTTTCGCTTTT
AACGCTGCGGAGCAACTAAT

Product: regulatory protein Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASEALAAGFRPCKRCQPDKANAQ
QHRLDKITHACRLLEQETPVTLEALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADSAPADL
TFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIP
CHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER

Sequences:

>Translated_354_residues
MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASEALAAGFRPCKRCQPDKANAQ
QHRLDKITHACRLLEQETPVTLEALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADSAPADL
TFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIP
CHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER
>Mature_354_residues
MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASEALAAGFRPCKRCQPDKANAQ
QHRLDKITHACRLLEQETPVTLEALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADSAPADL
TFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIP
CHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=31.4814814814815, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788542, Length=354, Percent_Identity=98.3050847457627, Blast_Score=722, Evalue=0.0,
Organism=Escherichia coli, GI1787596, Length=89, Percent_Identity=52.8089887640449, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=163, Percent_Identity=40.4907975460123, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115533070, Length=128, Percent_Identity=43.75, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320001, Length=179, Percent_Identity=31.2849162011173, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137554, Length=171, Percent_Identity=34.5029239766082, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39234; Mature: 39234

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASE
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEECCCHHHHHHHHHCCHHCCCCHH
ALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTLEALADHVAMSPFHLHRLF
HHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHH
KATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFPDSSSYYRKADETLGMTAKQF
HHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
RHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADSAPADL
HCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
TFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKP
CHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCH
KAVRAVASACAANKLAIVIPCHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER
HHHHHHHHHHHCCCEEEEEECHHHHCCCCCCCCEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASE
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEECCCHHHHHHHHHCCHHCCCCHH
ALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTLEALADHVAMSPFHLHRLF
HHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHH
KATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFPDSSSYYRKADETLGMTAKQF
HHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
RHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADSAPADL
HCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
TFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKP
CHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCH
KAVRAVASACAANKLAIVIPCHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER
HHHHHHHHHHHCCCEEEEEECHHHHCCCCCCCCEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]