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The map label for this gene is guaB [H]

Identifier: 170684009

GI number: 170684009

Start: 2716901

End: 2718436

Strand: Reverse

Name: guaB [H]

Synonym: EcSMS35_2657

Alternate gene names: 170684009

Gene position: 2718436-2716901 (Counterclockwise)

Preceding gene: 170680333

Following gene: 170683481

Centisome position: 53.64

GC content: 54.62

Gene sequence:

>1536_bases
ATGCAATCGGTTACGCTCTGTATAATGCCGCGGCAATATTTATTAACCACTCTGGTCGAGATATTGCCCATGCTACGTAT
CGCTAAAGAAGCTCTGACGTTTGACGACGTTCTCCTCGTTCCTGCTCATTCTACCGTTCTGCCGAATACTGCTGACCTCA
GCACCCAGCTGACGAAAACTATTCGTCTGAATATCCCTATGCTTTCCGCAGCAATGGATACCGTAACGGAAGCGCGCCTG
GCTATTGCTCTGGCTCAGGAAGGCGGTATCGGCTTTATCCACAAAAACATGTCCATTGAACGCCAGGCAGAAGAAGTTCG
CCGTGTGAAAAAACACGAATCTGGCGTGGTAACTGATCCGCAGACCGTGCTGCCGACAACCACCCTGCGTGAAGTGAAAG
AACTGACCGAGCGTAACGGCTTTGCGGGCTACCCGGTGGTTACCGAAGAAAACGAACTGGTCGGCATCATCACCGGTCGT
GACGTGCGTTTTGTGACCGATCTGAATCAACCGGTTAGCGTTTACATGACGCCGAAAGAGCGTCTGGTAACCGTGCGTGA
AGGCGAAGCCCGTGAAGTGGTGCTGGCAAAAATGCACGAAAAACGCGTTGAAAAGGCGCTGGTGGTTGATGACGAATTCC
ACCTGATCGGCATGATCACTGTGAAGGACTTCCAGAAAGCGGAACGTAAACCGAACGCGTGTAAAGACGAACAAGGCCGT
CTGCGTGTAGGTGCAGCGGTTGGCGCGGGTGCGGGTAACGAAGAGCGTGTTGATGCGCTGGTTGCCGCAGGCGTTGACGT
TCTGCTGATCGACTCCTCTCACGGTCACTCTGAAGGTGTTCTGCAGCGTATCCGTGAAACTCGCGCTAAATATCCTGACC
TGCAAATCATCGGCGGCAACGTGGCAACAGCAGCAGGTGCCCGCGCGCTGGCAGAAGCCGGTTGCAGTGCCGTTAAAGTG
GGTATCGGCCCTGGTTCTATTTGTACTACCCGCATTGTTACTGGCGTCGGTGTTCCGCAGATCACCGCCGTTGCTGACGC
AGTAGAAGCCCTGGAAGGCACCGGAATTCCGGTTATCGCTGACGGTGGTATTCGTTTCTCCGGCGACATCGCCAAAGCTA
TCGCCGCTGGCGCAAGCGCGGTAATGGTGGGTTCCATGCTGGCAGGTACTGAAGAATCTCCGGGTGAAATTGAACTCTAC
CAGGGCCGTTCTTACAAATCTTACCGTGGTATGGGTTCCCTGGGCGCGATGTCCAAAGGTTCTTCTGACCGTTACTTCCA
GAGCGACAACGCTGCCGACAAACTGGTGCCGGAAGGTATCGAAGGTCGCGTGGCCTATAAAGGTCGCCTGAAAGAGATCA
TTCACCAGCAGATGGGCGGCCTGCGCTCCTGTATGGGCCTGACCGGCTGTGGTACTATCGACGAACTGCGTACTAAAGCG
GAGTTTGTACGTATCAGCGGTGCGGGCATTCAGGAAAGCCACGTTCACGACGTGACCATTACTAAAGAGTCCCCGAACTA
CCGTCTGGGCTCCTGA

Upstream 100 bases:

>100_bases
GGTAACATGTGAGCGAGATCAAATTCTAAATCAGCAGGTTATTCAGTCGATAGTAACCCGCCCTCCGGGGATAGCAAGCA
TTTTTTGCAAAAAGGGGTAG

Downstream 100 bases:

>100_bases
TTCTCTTCGCCCGACTTCATGTCGGGCGATTTATATTATCTGTTTCACTTGCCTCGGAATAAGCGTCAATGACGGAAAAC
ATTCATAAGCATCGCATCCT

Product: inosine 5'-monophosphate dehydrogenase

Products: NA

Alternate protein names: IMP dehydrogenase; IMPD; IMPDH [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MQSVTLCIMPRQYLLTTLVEILPMLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARL
AIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGR
DVRFVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGR
LRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKV
GIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELY
QGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKA
EFVRISGAGIQESHVHDVTITKESPNYRLGS

Sequences:

>Translated_511_residues
MQSVTLCIMPRQYLLTTLVEILPMLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARL
AIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGR
DVRFVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGR
LRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKV
GIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELY
QGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKA
EFVRISGAGIQESHVHDVTITKESPNYRLGS
>Mature_511_residues
MQSVTLCIMPRQYLLTTLVEILPMLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARL
AIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGR
DVRFVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGR
LRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKV
GIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELY
QGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKA
EFVRISGAGIQESHVHDVTITKESPNYRLGS

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains [H]

Homologues:

Organism=Homo sapiens, GI66933016, Length=460, Percent_Identity=41.9565217391304, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI217035146, Length=466, Percent_Identity=41.4163090128755, Blast_Score=326, Evalue=4e-89,
Organism=Homo sapiens, GI34328928, Length=466, Percent_Identity=41.2017167381974, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI34328930, Length=466, Percent_Identity=41.2017167381974, Blast_Score=325, Evalue=8e-89,
Organism=Homo sapiens, GI156616279, Length=466, Percent_Identity=41.2017167381974, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI217035152, Length=455, Percent_Identity=41.5384615384615, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI217035148, Length=466, Percent_Identity=40.343347639485, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI217035150, Length=466, Percent_Identity=37.7682403433476, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI156104880, Length=267, Percent_Identity=34.4569288389513, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI50541956, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50541954, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50541952, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50541948, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1788855, Length=488, Percent_Identity=100, Blast_Score=984, Evalue=0.0,
Organism=Escherichia coli, GI1786293, Length=221, Percent_Identity=36.6515837104072, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994385, Length=478, Percent_Identity=36.4016736401674, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71994389, Length=423, Percent_Identity=38.7706855791962, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17560440, Length=219, Percent_Identity=36.986301369863, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6322012, Length=486, Percent_Identity=39.3004115226337, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6323585, Length=465, Percent_Identity=40, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6323464, Length=448, Percent_Identity=41.5178571428571, Blast_Score=313, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6319352, Length=342, Percent_Identity=39.766081871345, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6319353, Length=118, Percent_Identity=40.6779661016949, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24641071, Length=487, Percent_Identity=41.2731006160164, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24641073, Length=487, Percent_Identity=41.2731006160164, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI28571163, Length=445, Percent_Identity=41.7977528089888, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR005990
- InterPro:   IPR018529
- InterPro:   IPR015875
- InterPro:   IPR001093 [H]

Pfam domain/function: PF00571 CBS; PF00478 IMPDH [H]

EC number: =1.1.1.205 [H]

Molecular weight: Translated: 54669; Mature: 54669

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVTLCIMPRQYLLTTLVEILPMLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKT
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHEEEE
IRLNIPMLSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDP
EEECCCHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
QTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKE
CHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEEECCHH
RLVTVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGR
HEEEEECCCCHHHHHHHHHHHHHHHEEEECCCEEEEEEEEEHHHHHHHCCCCCCCCCCCC
LRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGN
EEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCC
VATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIA
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
DGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKG
CCCEEECCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCC
SSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKA
CCCCCCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHH
EFVRISGAGIQESHVHDVTITKESPNYRLGS
HEEEEECCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MQSVTLCIMPRQYLLTTLVEILPMLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKT
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHEEEE
IRLNIPMLSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDP
EEECCCHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
QTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKE
CHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEEECCHH
RLVTVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGR
HEEEEECCCCHHHHHHHHHHHHHHHEEEECCCEEEEEEEEEHHHHHHHCCCCCCCCCCCC
LRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGN
EEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCC
VATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIA
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
DGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKG
CCCEEECCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCC
SSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKA
CCCCCCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHH
EFVRISGAGIQESHVHDVTITKESPNYRLGS
HEEEEECCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]