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The map label for this gene is mdtA [H]

Identifier: 170683959

GI number: 170683959

Start: 999683

End: 1000930

Strand: Reverse

Name: mdtA [H]

Synonym: EcSMS35_0986

Alternate gene names: 170683959

Gene position: 1000930-999683 (Counterclockwise)

Preceding gene: 170682554

Following gene: 170680654

Centisome position: 19.75

GC content: 54.97

Gene sequence:

>1248_bases
ATGAAAGGCAGTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCA
AGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGGTGGTCGCCGTGGTA
TGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCTGTTCCGCGTTACCTCACCGGGCTTGGC
ACCATTACCGCCGCTAATACTGTTACGGTGCGCAGCCGCGTGGACGGTCAACTGATGGCGTTGCATTTCCAGGAAGGCCA
GCAGGTCAAAGCTGGCGATTTATTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCTGGGCCAAC
TGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGTAAAAACCAATCTCGTA
TCTCGTCAGGAACTGGATGCCCAACAAGCGCTGGTCAGTGAAACCGAAGGTACCATTAAGGCTGATGAAGCAAGCGTCGC
CAGCGCGCAATTGCAACTCGACTGGAGCCGTATCACCGCACCAGTTGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTG
GTAACCAAATCTCCAGTGGCGACACCACCGGGATTGTGGTGATCACCCAAACACATCCTATCGATCTGGTCTTTACTCTG
CCGGAAAGCGATATCGCCACCGTTGTACAGGCACAAAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAA
CTCGAAGAAGTTAAGTGAAGGCACGCTGTTAAGCCTTGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAG
CTCGCTTTAATAATCAGGATGATGCGCTTTTCCCTAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCGAACAAAAC
GCCGTGGTGATCCCCACCGCCGCTCTGCAAATGGGCAACGAAGGCCATTTTGTCTGGGTGCTGAATAGCGAAAACAAGGT
CAGCAAACATCTGGTGACACCGGGCATTCAGGACAGTCAGAGAGTGGTGATCCGCGCAGGTATTTCTGCGGGCGATCGCG
TGGTGACGGATGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACCACTTCGGAA
GAGAAAGCCACCAGCCGCGAATACGCGAAAAAAGGAGCACGCTCCTGA

Upstream 100 bases:

>100_bases
ATTCCCGATAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTC
AGGAAGAGAAACTCTTAACG

Downstream 100 bases:

>100_bases
TGCAGGTGTTACCCCCGAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTCGCCACCACGCTGCTGATG
GTGGCGATCTTACTCGCCGG

Product: multidrug efflux system subunit MdtA

Products: NA

Alternate protein names: Multidrug transporter mdtA [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLG
TITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQALGQLAKDKATLANARRDLARYQQLVKTNLV
SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTL
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQN
AVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQRVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTSE
EKATSREYAKKGARS

Sequences:

>Translated_415_residues
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLG
TITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQALGQLAKDKATLANARRDLARYQQLVKTNLV
SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTL
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQN
AVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQRVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTSE
EKATSREYAKKGARS
>Mature_415_residues
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLG
TITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQALGQLAKDKATLANARRDLARYQQLVKTNLV
SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTL
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQN
AVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQRVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTSE
EKATSREYAKKGARS

Specific function: The mdtABC tripartite complex confers resistance against novobiocin and deoxycholate [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=415, Percent_Identity=98.7951807228916, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1789929, Length=323, Percent_Identity=28.1733746130031, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786668, Length=321, Percent_Identity=29.595015576324, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1789665, Length=330, Percent_Identity=28.7878787878788, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081796, Length=394, Percent_Identity=26.1421319796954, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081951, Length=161, Percent_Identity=29.8136645962733, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022824
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44514; Mature: 44514

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQSPAGGRRGMRSGPLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
PVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
CHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEEEEEEECCCCEECCCCEEECCCH
SQFKVALAQALGQLAKDKATLANARRDLARYQQLVKTNLVSRQELDAQQALVSETEGTIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTL
ECCCHHEEEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCEEEEEEC
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQD
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCC
DALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQ
CCCCCHHHCCCEEEEECCCCEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCC
RVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTSEEKATSREYAKKGARS
EEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQSPAGGRRGMRSGPLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
PVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
CHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEEEEEEECCCCEECCCCEEECCCH
SQFKVALAQALGQLAKDKATLANARRDLARYQQLVKTNLVSRQELDAQQALVSETEGTIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTL
ECCCHHEEEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCCEEEEEEC
PESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQD
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCC
DALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQ
CCCCCHHHCCCEEEEECCCCEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCC
RVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTSEEKATSREYAKKGARS
EEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA