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The map label for this gene is yfiE [H]

Identifier: 170683897

GI number: 170683897

Start: 2809484

End: 2810365

Strand: Reverse

Name: yfiE [H]

Synonym: EcSMS35_2730

Alternate gene names: 170683897

Gene position: 2810365-2809484 (Counterclockwise)

Preceding gene: 170680667

Following gene: 170679700

Centisome position: 55.45

GC content: 49.09

Gene sequence:

>882_bases
ATGGATCTGCGCCGTTTTATTACACTTAAAACCGTGGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTG
CACACAATCGACGGTGACTTTTCATATTCAGCAGCTTGAGCAGGAGTTCTCAGTCCAGTTATTTGAGAAAATTGGTCGAC
GAATGTGCCTTACCCGCGAAGGAAAAAAGCTGCTGCCGCACATCTATGAACTCACCAGAGTGATGGATACCCTTCGTGAG
GCTGCCAAAAAAGAGTCGGATCCGGACGGGGAACTACGCGTTGTTTCAGGCGAAACGTTGCTTTCTTACCGAATGCCGCA
GGTGTTGCAGCGTTTTCGGCAACGCGCACCAAAAGTGTGTTTATCGTTGCAGTCTCTGAACTGTTACGTGATCCGTGATG
CGCTACTGAATGATGAGGCTGATGTCGGCGTCTTTTACCGCGTAGGGAATGATGATGCTCTGAACCGACGAGAGTTGGGT
GAACAATCGCTGGTGCTGGTAGCTTCACCGCAAATTGCAGATGTCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAG
TTTTATTATCAATGAACCGCAATGTGTCTTCCGCCAGATATTTGAGAGCACGCTGCGCCAGCGACGGATCACGGTGGAAA
ACACCATTGAGCTTATAAGTATCGAAAGCATCAAGCGTTGTGTCGCGGCAAATATCGGGGTCAGTTACCTGCCGCGCTTT
ACAGTGGCAAATGAACTTGCGAGTGGCGAATTAATCGAATTGCCTTTTGGCGAACAGTCGCAGACCATTACGGCAATGTG
CGCTCACCATGCCGGAAAAGCGGTTAGCCCGGCGATGCACATTTTTATTCAGTGTGTTGAAGAGTGTTTTTTGCCGGGAT
AA

Upstream 100 bases:

>100_bases
GTTATCTCCTCTGTTTTGGTAGACAGTACCCGACACGAAACATTTAAAAAAATGAATGTTTTAATATTCTAAATTCGATA
TTTTCGATAGGTTTGGGGTT

Downstream 100 bases:

>100_bases
AAATAATGCCGGGCTTAAACCCGGCATTGAGATTACTCTTCTGTCGTTTGCGGTGGCACGCCCGTTCCGCTCGGTTTACG
GCGCTTACCAATATTTTTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVCLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
TVANELASGELIELPFGEQSQTITAMCAHHAGKAVSPAMHIFIQCVEECFLPG

Sequences:

>Translated_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVCLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
TVANELASGELIELPFGEQSQTITAMCAHHAGKAVSPAMHIFIQCVEECFLPG
>Mature_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVCLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
TVANELASGELIELPFGEQSQTITAMCAHHAGKAVSPAMHIFIQCVEECFLPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=96.9283276450512, Blast_Score=579, Evalue=1e-167,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=25.4125412541254, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=24.0418118466899, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786401, Length=313, Percent_Identity=26.8370607028754, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30.625, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=25.7042253521127, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=21.3991769547325, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=22.9681978798587, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=24, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33201; Mature: 33201

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLVLVASPQIADVDFTEP
HHHHCCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHH
TVANELASGELIELPFGEQSQTITAMCAHHAGKAVSPAMHIFIQCVEECFLPG
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLVLVASPQIADVDFTEP
HHHHCCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHH
TVANELASGELIELPFGEQSQTITAMCAHHAGKAVSPAMHIFIQCVEECFLPG
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]