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The map label for this gene is ybfF [H]

Identifier: 170683869

GI number: 170683869

Start: 711283

End: 712047

Strand: Reverse

Name: ybfF [H]

Synonym: EcSMS35_0708

Alternate gene names: 170683869

Gene position: 712047-711283 (Counterclockwise)

Preceding gene: 170680055

Following gene: 170681944

Centisome position: 14.05

GC content: 50.59

Gene sequence:

>765_bases
ATGAAATTGAATATCCGCGCGCAAACTGCACAAAACCAGCACAATAATTCTCCCATCGTTCTTGTCCATGGTCTGTTTGG
CAGCCTCGACAACCTTGGCGTACTGGCTCGCGATCTGGTAAACGATCACAATATCATCCAGGTTGATATGCGTAACCACG
GTCTTTCACCGAGAGATCCGGTAATGAATTACCCGGCGATGGCGCAGGATCTTGTTGATACCCTGGATGCACAGCAGATC
GACAAAGCAACATTTATCGGTCACTCCATGGGCGGTAAAGCGGTAATGGCTCTTACTGCACTAGCCCCCGATCGCATTGA
CAAGCTGGTGGCGATTGATATTGCGCCGGTCGACTATCACGTACGCCGTCATGATGAGATTTTCGCGGCCATCAACGCAG
TCAGTGAATCAGACGCACAAACACGCCAGCAAGCAGCGGCAATAATGCGCCAGCATCTTAATGAAGAAGGGGTGATTCAG
TTTCTGTTGAAATCTTTTGTTGACGGGGAGTGGCGCTTTAACGTGCCAGTATTGTGGGATCAGTATCCGCATATTGTGGG
TTGGGAGAAAATCCCGGCATGGGATCATCCTACCCTGTTTATCCCTGGCGGCAATTCTCCGTATGTTAGCGAGCAGTACC
GTGATGATTTACTGGCACAATTTCCACAGGCTCGGGCGCATGTGATTGCAGGCGCGGGTCACTGGGTCCATGCTGAAAAA
CCGGATGCGGTATTACGCGCTATCCGTCGCTATCTCAATGATTAA

Upstream 100 bases:

>100_bases
CTTGCAGAAAAACTCGGAATAAACCTTATTTGTTGCAGCCCTGTGGCACAATGGTCAACATAATACCCTAACCCGACAAC
GCGAAAAAAAGAACTTTGCC

Downstream 100 bases:

>100_bases
CGCAACTGATTAAAAACCCAGCGTCCGCGTCGCTTTCCGCGCGTGGGCGTTGGCGTGACTCATCGCGCTGATGTATTATG
GCGCGCTATCCATCCGGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQI
DKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEK
PDAVLRAIRRYLND

Sequences:

>Translated_254_residues
MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQI
DKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEK
PDAVLRAIRRYLND
>Mature_254_residues
MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQI
DKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEK
PDAVLRAIRRYLND

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=246, Percent_Identity=34.9593495934959, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI23200008, Length=246, Percent_Identity=34.9593495934959, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI223941839, Length=239, Percent_Identity=31.3807531380753, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786902, Length=254, Percent_Identity=99.2125984251969, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17508535, Length=254, Percent_Identity=33.8582677165354, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17507063, Length=256, Percent_Identity=27.734375, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321468, Length=261, Percent_Identity=31.4176245210728, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640348, Length=272, Percent_Identity=29.0441176470588, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646055, Length=263, Percent_Identity=24.3346007604563, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28478; Mature: 28478

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDP
CEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
VMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYH
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCEEEEEEECCCHH
VRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQFLLKSFVDGEWRFNVPVLWD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEC
QYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEK
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHEEEEECCCCEEECCC
PDAVLRAIRRYLND
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDMRNHGLSPRDP
CEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
VMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDYH
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCEEEEEEECCCHH
VRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQFLLKSFVDGEWRFNVPVLWD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEC
QYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEK
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHEEEEECCCCEEECCC
PDAVLRAIRRYLND
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]