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The map label for this gene is thiG [H]

Identifier: 170683844

GI number: 170683844

Start: 4536396

End: 4537211

Strand: Reverse

Name: thiG [H]

Synonym: EcSMS35_4438

Alternate gene names: 170683844

Gene position: 4537211-4536396 (Counterclockwise)

Preceding gene: 170682128

Following gene: 170681122

Centisome position: 89.52

GC content: 57.23

Gene sequence:

>816_bases
ATGGCGACCAGATCCTGCTTTTTCAAGTTATTGCAGGGGGTTGAGATGTTACATATTGCGAACAAAACGTTTGATTCACA
TCTGTTTACTGGCACCGGAAAATTCGCCTCTTCACAACTGATGGTGGAGTCTATTCGCGCTTCCGGCAGCCAACTGGTGA
CGCTGGCGATGAAACGCGTCAACTTGCGCCAGCACAACGACGCTATCCTCGAACCGCTTATCGCGGCGGGTGTGACCCTG
CTGCCAAATACATCCGGGGCGAAAACAGCGGAAGAAGCCATTTTCGCCGCCCATCTGGCTCGTGAAGCGTTAGGCACAAA
CTGGTTAAAATTAGAGATTCACCCTGACGCCCGCTGGCTGTTGCCCGATCCCATCGAAACCCTGAAAGCCGCCGAAACGC
TGGTACAACAGGGATTTGTCGTGCTGCCTTACTGCGGGGCCGATCCGGTATTGTGTAAACGTCTGGAAGAAGTCGGCTGT
GCAGCGGTGATGCCGCTCGGCGCGCCGATTGGCTCGAATCAGGGACTGGAAACCCGTGCCATGCTGGAGATTATTATCCA
GCAAGCCACGGTTCCGGTGGTTGTCGATGCTGGCATCGGCGTTCCCAGCCATGCCGCGCAGGCGCTGGAAATGGGGGCCG
ACGCGGTGTTAGTGAATACGGCGATTGCCGTCGCGGACGATCCCGTCAACATGGCGAAGGCATTTCGTCTGGCAGTAGAA
GCAGGCCTGCTGGCACGTCAGTCCGGACCGGGCAGCCGCAGTCATTTTGCTCATGCCACCAGCCCGCTGACCGGATTTCT
GGAGGCATCGGCATGA

Upstream 100 bases:

>100_bases
GAACTGCTGGAGCAACTCGATCAACAACAAGCAGGTGCGGCACTGGCGATTAATCAGCAAATCGTCCCGCGGGAACAATG
GGCGCAACATATCGTGCAGG

Downstream 100 bases:

>100_bases
AAAACTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTA
GAGCGGGCGCTAAATGCCTC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MATRSCFFKLLQGVEMLHIANKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKRVNLRQHNDAILEPLIAAGVTL
LPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGC
AAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
AGLLARQSGPGSRSHFAHATSPLTGFLEASA

Sequences:

>Translated_271_residues
MATRSCFFKLLQGVEMLHIANKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKRVNLRQHNDAILEPLIAAGVTL
LPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGC
AAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
AGLLARQSGPGSRSHFAHATSPLTGFLEASA
>Mature_270_residues
ATRSCFFKLLQGVEMLHIANKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKRVNLRQHNDAILEPLIAAGVTLL
PNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCA
AVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVEA
GLLARQSGPGSRSHFAHATSPLTGFLEASA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=256, Percent_Identity=98.046875, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28577; Mature: 28446

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRSCFFKLLQGVEMLHIANKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKRV
CCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
NLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWL
HHHHCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
LPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQGLETRA
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHH
MLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHH
AGLLARQSGPGSRSHFAHATSPLTGFLEASA
HHHHHHCCCCCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
ATRSCFFKLLQGVEMLHIANKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKRV
CHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
NLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWL
HHHHCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
LPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQGLETRA
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHH
MLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHH
AGLLARQSGPGSRSHFAHATSPLTGFLEASA
HHHHHHCCCCCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA