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The map label for this gene is insD1 [C]

Identifier: 170683732

GI number: 170683732

Start: 3206914

End: 3207357

Strand: Reverse

Name: insD1 [C]

Synonym: EcSMS35_3118

Alternate gene names: 170683732

Gene position: 3207357-3206914 (Counterclockwise)

Preceding gene: 170683540

Following gene: 170681617

Centisome position: 63.28

GC content: 53.6

Gene sequence:

>444_bases
ATGCCGGGCAACTGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGCTATGTCGGGGCTAAAT
CGCGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGCATAGCAGAG
AGCTTCGTGAAAACGATAAAGCGTGATTACATCAGTATCATGCCCAAACCAGACGGGTTAATGGCAGCAAAGAACCTTGC
AGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCACTGGGTTATCGCTCGCCACGGGAATATCTACGGCAGC
GGGCCAGTAATGGGTTAAGTGATAACAGGTGTCTGGAAATATAG

Upstream 100 bases:

>100_bases
GCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTTGG

Downstream 100 bases:

>100_bases
GGGCAAATCCACTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGAC
GAAGCGATAGCGATAATGAC

Product: IS2 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MPGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQFARMLGLEPKNTAVRSPESNGIAE
SFVKTIKRDYISIMPKPDGLMAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRASNGLSDNRCLEI

Sequences:

>Translated_147_residues
MPGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQFARMLGLEPKNTAVRSPESNGIAE
SFVKTIKRDYISIMPKPDGLMAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRASNGLSDNRCLEI
>Mature_146_residues
PGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQFARMLGLEPKNTAVRSPESNGIAES
FVKTIKRDYISIMPKPDGLMAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRASNGLSDNRCLEI

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=99, Percent_Identity=92.9292929292929, Blast_Score=193, Evalue=5e-51,
Organism=Escherichia coli, GI1789424, Length=99, Percent_Identity=92.9292929292929, Blast_Score=193, Evalue=5e-51,
Organism=Escherichia coli, GI1789224, Length=99, Percent_Identity=92.9292929292929, Blast_Score=193, Evalue=5e-51,
Organism=Escherichia coli, GI1788305, Length=99, Percent_Identity=92.9292929292929, Blast_Score=193, Evalue=5e-51,
Organism=Escherichia coli, GI1787669, Length=99, Percent_Identity=92.9292929292929, Blast_Score=193, Evalue=5e-51,
Organism=Escherichia coli, GI1786558, Length=99, Percent_Identity=92.9292929292929, Blast_Score=193, Evalue=5e-51,
Organism=Escherichia coli, GI1789853, Length=56, Percent_Identity=98.2142857142857, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1788203, Length=56, Percent_Identity=98.2142857142857, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1786203, Length=56, Percent_Identity=98.2142857142857, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1787223, Length=56, Percent_Identity=92.8571428571429, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1786470, Length=56, Percent_Identity=92.8571428571429, Blast_Score=115, Evalue=9e-28,
Organism=Escherichia coli, GI1786459, Length=56, Percent_Identity=92.8571428571429, Blast_Score=115, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 16720; Mature: 16589

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQFARM
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLMAAKNLAEAFEHYNEWHPHS
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ALGYRSPREYLRQRASNGLSDNRCLEI
CCCCCCHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
PGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQFARM
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLMAAKNLAEAFEHYNEWHPHS
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ALGYRSPREYLRQRASNGLSDNRCLEI
CCCCCCHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA