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The map label for this gene is iucA [H]

Identifier: 170683655

GI number: 170683655

Start: 3263480

End: 3265204

Strand: Reverse

Name: iucA [H]

Synonym: EcSMS35_3182

Alternate gene names: 170683655

Gene position: 3265204-3263480 (Counterclockwise)

Preceding gene: 170681895

Following gene: 170681805

Centisome position: 64.42

GC content: 54.14

Gene sequence:

>1725_bases
ATGATCCTGCCCTCTGAAAAATCCGCCACAGATGTGGCTGCGCAGTGCTTCCTGAATGCCTTGATTCGTGAAACCAAAGA
CTGGCAACTGGCGGAATACCCGCCAGACGAATTGATTATCCCGCTGGACGAACAGAAGTCGCTCCATTTCAGAGTGGCTT
ATTTCTCCCCAACCCAGCATCACCGCTTTGCATTTCCTGCTCATCTGGTCACGGCATCAGGCAGTTATCCTGTCGATTTT
ACCACTCTCTCCCGGCTTATTATTGACAAGCTCCGGCATCAACTTTTTCTGCCAGTTCCCCTCTGCGAAACTTTCCACCA
ACGCGTGCTGGAAAGCTACGCCCATACGCAACAGACAATTGATGCCCGCCATGACTGGGCCATCCTGCGTGAGAAAGCGT
TGAATTTTGGCGAGGCTGAGCAGGCACTGCTGACAGGACACGCTTTCCACCCTGCACCTAAGTCTCATGAACCGTTTAAC
CGCCAGGAAGCTGAACGATACCTGCCTGACATGGCACCTCACTTCCCGCTGCGGTGGTTTTCGGTGGATAAAACGCAAAT
CGCTGGTGAAAGTCTGCATCTTAACCTTCAACAGCGGTTGACGCGATTTGCCGCAGAAAATGCGCCCCAGTTACTCAACG
AATTAAGTGACAATCAATGGCTGTTCCCGCTGCACCCGTGGCAGGGAGAATATCTTTTGCAGCAGGTGTGGTGCCAGGCA
CTTTTTGCTAAAGGACTTATCAGAGACTTAGGCGAGGCCGGCACGTCGTGGCTGCCGACCACCTCTTCCCGCTCTCTCTA
CTGTGCTACCAGCCGCGATATGATCAAGTTCTCCCTGAGCGTTCGGCTGACCAACTCCGTCCGTACTCTGTCTGTGAAAG
AGGTGGAGCGAGGAATGCGCCTGGCACGTCTGGCGCAAACCGACGGCTGGCAGATGCTACAGGCCCGCTTCCCTACTTTC
CGGGTAATGCAGGAGGACGGCTGGGCCGGGCTGCGCGATCTTAACGGCAACATCATGCAGGAAAGTTTGTTTTCCCTGCG
TGAAAACCTGCTGCTGGAGCAGCCGCAAAGCCAGACCAACGTTCTGGTTTCCCTGACTCAGGCCGCACCGGATGGCGGTG
ATTCGCTGCTGGTTTCGGCGGTAAAACGCCTGAGCGATCGCCTCGGTATCACTGTGCAACAGGCCGCCCACGCATGGGTC
GATGCTTACTGTCAGCAGGTGCTGAAGCCTCTGTTTACGGCTGAAGCGGATTACGGCCTGGTGCTGCTGGCGCATCAGCA
AAATATTCTTGTGCAGATGCTTGGGGATCTGCCGGTCGGATTTATTTACCGTGACTGCCAGGGCAGCGCGTTTATGCCTC
ACGCGACAGAGTGGCTCGATACCATTGACGAGGCGCAGGCGGAAAATATTTTCACCCGTGAGCAGTTGCTGCGCTATTTC
CCTTATTACCTGCTGGTTAACTCCACTTTTGCCGTAACTGCTGCGCTGGGTGCTGCCGGGCTGGACAGCGAAGCGAATCT
GATGGCTCGTGTACGAACATTGCTGGCTGAAGTGCGTGACCAGGTGACTCATAAAACCTGTCTCAACTATGTTCTGGAAA
GTCCGTACTGGAACGTAAAAGGTAACTTTTTCTGTTATCTGAACGATCATAACGAGAACACCATCGTTGACCCTTCGGTG
ATCTATTTTGATTTCGCTAACCCGCTGCAGGCTCAGGAGGTCTGA

Upstream 100 bases:

>100_bases
TTTGTAAAATTAATTTTTCTGACAATAATATTTCTCATTGATAATGATAATCATTATTGACATAATTGTTATTATTTTAC
TGTGTAGGAGCTGTTTGATT

Downstream 100 bases:

>100_bases
ATGTCTGGGGCAAACATTGTTCACAGCGGATATGGACTGCGCTGTGAAAAACTCGACAAGCCTCTGAATCTTGGCTGGGG
GCTGGACAATAGCGCGGTGT

Product: aerobactin siderophore biosynthesis protein IucA

Products: ADP; phosphate; aerobactin

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDF
TTLSRLIIDKLRHQLFLPVPLCETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPLHPWQGEYLLQQVWCQA
LFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTF
RVMQEDGWAGLRDLNGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWV
DAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATEWLDTIDEAQAENIFTREQLLRYF
PYYLLVNSTFAVTAALGAAGLDSEANLMARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSV
IYFDFANPLQAQEV

Sequences:

>Translated_574_residues
MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDF
TTLSRLIIDKLRHQLFLPVPLCETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPLHPWQGEYLLQQVWCQA
LFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTF
RVMQEDGWAGLRDLNGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWV
DAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATEWLDTIDEAQAENIFTREQLLRYF
PYYLLVNSTFAVTAALGAAGLDSEANLMARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSV
IYFDFANPLQAQEV
>Mature_574_residues
MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDF
TTLSRLIIDKLRHQLFLPVPLCETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPLHPWQGEYLLQQVWCQA
LFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTF
RVMQEDGWAGLRDLNGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWV
DAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATEWLDTIDEAQAENIFTREQLLRYF
PYYLLVNSTFAVTAALGAAGLDSEANLMARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSV
IYFDFANPLQAQEV

Specific function: Unknown

COG id: COG4264

COG function: function code Q; Siderophore synthetase component

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iucA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007310
- InterPro:   IPR022770 [H]

Pfam domain/function: PF06276 FhuF; PF04183 IucA_IucC [H]

EC number: 6.3.2.27

Molecular weight: Translated: 65350; Mature: 65350

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQKSLHFRVAYFSPTQH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEECCCCCCEEEEEEEECCCCC
HRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFHQRVLESYAHTQQTI
CCEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
DARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFNRQEAERYLPDMAPHFPLRWF
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEE
SVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPLHPWQGEYLLQQVWCQA
ECCHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
LFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSVRTLSVKEVERGMR
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCCEEEHHHHHHHHH
LARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDLNGNIMQESLFSLRENLLLEQPQSQTN
HHHHHHCCCHHHHHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
VLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGL
EEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATEWLDTIDEAQAENIFTREQLLRYF
EEEEECHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PYYLLVNSTFAVTAALGAAGLDSEANLMARVRTLLAEVRDQVTHKTCLNYVLESPYWNVK
CHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
GNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV
CCEEEEEECCCCCEEECCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQKSLHFRVAYFSPTQH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEECCCCCCEEEEEEEECCCCC
HRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFHQRVLESYAHTQQTI
CCEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
DARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFNRQEAERYLPDMAPHFPLRWF
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEE
SVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPLHPWQGEYLLQQVWCQA
ECCHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
LFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNSVRTLSVKEVERGMR
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCCEEEHHHHHHHHH
LARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDLNGNIMQESLFSLRENLLLEQPQSQTN
HHHHHHCCCHHHHHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
VLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGL
EEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATEWLDTIDEAQAENIFTREQLLRYF
EEEEECHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PYYLLVNSTFAVTAALGAAGLDSEANLMARVRTLLAEVRDQVTHKTCLNYVLESPYWNVK
CHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
GNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV
CCEEEEEECCCCCEEECCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; citrate; N6-acetyl-N6-hydroxylysine

Specific reaction: 4 ATP + citrate + N6-acetyl-N6-hydroxylysine = 4 ADP + 4 phosphate + aerobactin

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8003107 [H]