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The map label for this gene is artI [H]

Identifier: 170683620

GI number: 170683620

Start: 894654

End: 895385

Strand: Reverse

Name: artI [H]

Synonym: EcSMS35_0891

Alternate gene names: 170683620

Gene position: 895385-894654 (Counterclockwise)

Preceding gene: 170682260

Following gene: 170680675

Centisome position: 17.67

GC content: 49.45

Gene sequence:

>732_bases
ATGAAAAAAGTTCTGATTGCCGCGTTAATTGCAGGTTTTAGTCTTTCCGCCACAGCTGCCGAAACTATTCGTTTTGCTAC
CGAAGCCTCCTATCCTCCGTTTGAATCGATTGATGCAAACAACCAGATCGTTGGTTTTGACGTCGACCTGGCACAAGCGC
TGTGTAAAGAGATTGATGCAACCTGCACTTTCTCCAACCAGGCATTTGACAGGCTGATCCCAAGCCTGAAATTCCGTCGC
GTGGAAGCGGTGATGGCCGGGATGGATATCACCCCAGAACGTGAAAAGCAGGTGCTGTTTACCACCCCTTACTATGACAA
CTCTGCCCTGTTTGTGGGTCAGCAGGGTAAATACACCAGTGTTGATCAGCTGAAAGGCAAAAAAGTCGGCGTTCAGAACG
GGACGACACACCAGAAGTTCATTATGGATAAGCACCCGGAAATCACCACCGTTCCGTATGACAGCTACCAGAACGCAAAA
CTGGATCTGCAAAACGGTCGTATCGACAGCGTATTCGGTGACACCGCAGTGGTCACCGAGTGGCTGAAAGACAACCCGAA
ACTGGCGGCAGTGGGCGATAAAGTGACCGATAAAGATTACTTCGGTACTGGTCTCGGCATCGCGGTACGTCAGGGCAACA
CTGAACTGCAGCAGAAACTCAACACTGCGCTGGAAAAAGTGAAGAAAGATGGCACTTACGAAACCATCTACAACAAATGG
TTCCAGAAGTAA

Upstream 100 bases:

>100_bases
ACGGTCATATCGTAGAACAAGGCGACGCGAGCTGCTTTACCGAGCCGCAAACCGAAGCATTTAAAAACTATCTCTCTCAC
TAATATTCGGGATAACAACA

Downstream 100 bases:

>100_bases
TTCCTGATGAATGAATTTTTTCCTTTAGCAAGCGCCGCCGGGATGACCGTCGGCCTTGCCGTTTGTGCATTGATTGTCGG
GCTGGCGCTGGCGATGTTCT

Product: arginine ABC transporter periplasmic arginine-binding protein ArtI

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDATCTFSNQAFDRLIPSLKFRR
VEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
FQK

Sequences:

>Translated_243_residues
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDATCTFSNQAFDRLIPSLKFRR
VEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
FQK
>Mature_243_residues
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDATCTFSNQAFDRLIPSLKFRR
VEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
FQK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=99.1769547325103, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1787085, Length=241, Percent_Identity=63.9004149377593, Blast_Score=339, Evalue=9e-95,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=33.596837944664, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=38.4279475982533, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1788228, Length=252, Percent_Identity=30.1587301587302, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=33.6065573770492, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27029; Mature: 27029

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDA
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCC
TCTFSNQAFDRLIPSLKFRRVEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTS
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCC
VDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAKLDLQNGRIDSVFGDTAVVTE
HHHHCCCEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCHHHHHH
WLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
HHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
FQK
HCC
>Mature Secondary Structure
MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDA
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCC
TCTFSNQAFDRLIPSLKFRRVEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGKYTS
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCC
VDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAKLDLQNGRIDSVFGDTAVVTE
HHHHCCCEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCHHHHHH
WLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYETIYNKW
HHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
FQK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]