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The map label for this gene is garK [H]

Identifier: 170683586

GI number: 170683586

Start: 3500461

End: 3501606

Strand: Reverse

Name: garK [H]

Synonym: EcSMS35_3419

Alternate gene names: 170683586

Gene position: 3501606-3500461 (Counterclockwise)

Preceding gene: 170683197

Following gene: 170681106

Centisome position: 69.09

GC content: 53.05

Gene sequence:

>1146_bases
ATGAAAATCGTAATCGCCCCAGACTCTTATAAAGAAAGTTTATCTGCCAGCGAGGTTGCGCAGGCGATAGAAAAAGGATT
TCGGGAAATTTTTCCTGATGCACAGTACGTTTCTGTTCCTGTTGCCGATGGTGGCGAAGGAACGGTGGAAGCGATGATTG
CAGCCACCCAGGGTTCCGAACGTCACGCCTGGGTTACAGGGCCGCTGGGCGAGAAGGTGAATGCCAGTTGGGGGATCTCC
GGCGATGGCAAAACCGCGTTTATTGAAATGGCGGCGGCCAGTGGGCTGGAGCTGGTACCTGCGGAAAAACGTGATCCACT
CGTGACCACTTCACGCGGCACAGGCGAGTTAATTCTGCAGGCGCTGGAGAGCGGCGCGACAAACATTATTATCGGCATTG
GCGGTAGCGCTACAAATGATGGCGGTGCAGGCATGGTACAGGCGCTGGGGGCGAAATTATGCGACGCCAACGGCAATGAA
ATTGGTTTTGGCGGCGGTAGTCTTAATACTCTGAATGATATTGATATTTCCGGCCTCGATCCGCGCTTAAAAGATTGCGT
CATTCGCGTCGCTTGTGATGTCACCAATCCGCTGGTGGGCGATAACGGCGCATCGCGCATCTTTGGCCCACAAAAGGGAG
CCAGTGAAGCGATGATTGTTGAGCTGGACAATAACCTTTCTCACTATGCCGATGTCATTAAAAAAGCGCTGCATGTTGAT
GTGAAAGATGTCCCCGGCGCAGGAGCTGCGGGTGGTATGGGCGCGGCGCTAATGGCGTTTCTTGGTGCGGAACTGAAAAG
CGGTATTGAAATCGTCACGACGGCGCTGAATCTGGAGGAACATATTCACGATTGTACGCTGGTGATCACCGGTGAAGGGC
GTATTGACAGCCAGAGTATACACGGCAAGGTTCCGATTGGCGTCGCAAACGTGGCGAAAAAGTACCATAAACCGGTGATT
GGCATTGCGGGTAGCCTGACCAATGATGTTGGCGTTGTACATCAGCATGGCATTGATGCGGTCTTCAGCGTATTGACCAG
TATTGGTACGTTGGACGAAGCATTCCGCGGGGCTTATGACAATATTTACCGTGCTTCACGTAATATCGCCGCGACACTGG
CGATTGGAATGCGCAACGCGGGGTGA

Upstream 100 bases:

>100_bases
TTAATGACGTCGTGCCCGGATGGCGTATTGCAATCCGGGCCTGGAATCCAGGCCGAATAAGAGAAACGCCCTCCGGCGTC
ATGTGGTAACAGGCATAGGT

Downstream 100 bases:

>100_bases
CAAGGGCGCGCAAACCCTCTATACTGCGCGCCGAAGCTGACCAGACAGTCGCCGCTTCGTCGTCGTCCTCTTCGGGGGAG
ACGGGCGGAGGGGAGGAAAG

Product: glycerate kinase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGIS
GDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYADVIKKALHVD
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVI
GIAGSLTNDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNAG

Sequences:

>Translated_381_residues
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGIS
GDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYADVIKKALHVD
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVI
GIAGSLTNDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNAG
>Mature_381_residues
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGIS
GDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYADVIKKALHVD
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVI
GIAGSLTNDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNAG

Specific function: Unknown

COG id: COG1929

COG function: function code G; Glycerate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycerate kinase type-1 family [H]

Homologues:

Organism=Escherichia coli, GI145693185, Length=381, Percent_Identity=98.9501312335958, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI1786724, Length=376, Percent_Identity=56.1170212765958, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018193
- InterPro:   IPR004381 [H]

Pfam domain/function: PF02595 Gly_kinase [H]

EC number: =2.7.1.31 [H]

Molecular weight: Translated: 39166; Mature: 39166

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGSE
CEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCC
RHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQ
CCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCEEEECCCHHHHHHH
ALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNEIGFGGGSLNTLNDIDISGLD
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
PRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYADVIKKALHVD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCEEEEEEECCCCCCCCCC
HGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYD
CCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
NIYRASRNIAATLAIGMRNAG
HHHHHCCCEEEEEEEECCCCC
>Mature Secondary Structure
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGSE
CEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCC
RHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQ
CCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCEEEECCCHHHHHHH
ALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNEIGFGGGSLNTLNDIDISGLD
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
PRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYADVIKKALHVD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCEEEEEEECCCCCCCCCC
HGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYD
CCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
NIYRASRNIAATLAIGMRNAG
HHHHHCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705543; 9278503 [H]