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The map label for this gene is yfiF

Identifier: 170683534

GI number: 170683534

Start: 2812535

End: 2813572

Strand: Reverse

Name: yfiF

Synonym: EcSMS35_2734

Alternate gene names: 170683534

Gene position: 2813572-2812535 (Counterclockwise)

Preceding gene: 170682366

Following gene: 170680667

Centisome position: 55.51

GC content: 56.36

Gene sequence:

>1038_bases
ATGAACGATGAAATGAAAGGTAAAAGCGGCAAGGTCAAAGTGATGTATGTCCGCAGTGATGATGATTCTGATAAACGTAC
CCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATG
ACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCACGGTTTCCCGCGCGCCGGGTGATGAGACG
CCGGAAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTTTTGCGTCGTCAGCGTGCGGAAGA
AACCCGCGTCTACGGAGAAAACGCCTGTCAGGCACTGTTCCAGAGCCGTCCGGAAGCGATTGTTCGCGCCTGGTTTATCC
AGAGCGTAACGCCACGTTTTAAAGAAGCCTTGCGCTGGATGGCGGCAAACCGCAAAGCGTACCATGTGGTGGATGAAGCG
GAACTGACAAAAGCGTCAGGCACGGAACATCACGGCGGCGTTTGCTTCCTGATCAAAAAGCGTAACGGTACAACCGTGCA
GCAGTGGGTAAGCCAGGCTGGCGCGCAGGATTGTGTACTGGCCCTGGAAAACGAATCTAACCCGCATAACCTGGGCGGCA
TGATGCGTAGCTGTGCGCACTTCGGCGTGAAAGGTGTTGTGGTGCAGGATGCAGCACTGCTGGAGTCTGGGGCGGCTATC
CGTACCGCAGAAGGCGGCGCAGAGCACGTTCAGCCGATTACTGGCGACAACATTGTTAACGTGCTGGATGATTTCCGTCA
GGCGGGTTACACCGTAGTGACAACTTCCAGCGAGCAGGGTAAACCGCTGTTCAAAACCAGTCTGCCAGCGAAAATGGTAC
TGGTGCTGGGTCAGGAATATGAAGGGTTACCGGATGCCGCACGCGATCCGAACGATCTGCGCGTGAAGATTGATGGTACT
GGCAACGTTGCCGGGCTGAATATCTCTGTCGCAACCGGCGTTCTTCTTGGCGAATGGTGGCGTCAGAATAAAGCCTGA

Upstream 100 bases:

>100_bases
TCAATGGGTATGATAGACACAGGCTTTGGAACGAAATCATGGCGAACCGCGCGGACTTCGGGTAATCTGCGCGCTTCGCG
CAGCGCTGGTGGAGAAAAGC

Downstream 100 bases:

>100_bases
TAAATCAGCCGGGTGGCAACTCTGCCATCCGGCATTTTCCCGCAAATTTACTCACTCTCTGCCGGTAATACTGGCATCCA
GTCAATCGGCGTCTCGCCAC

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Sequences:

>Translated_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA
>Mature_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1788935, Length=345, Percent_Identity=100, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1790623, Length=247, Percent_Identity=27.9352226720648, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFIF_ECO57 (P0AGJ6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G91059
- RefSeq:   NP_289139.1
- RefSeq:   NP_311474.1
- ProteinModelPortal:   P0AGJ6
- SMR:   P0AGJ6
- MINT:   MINT-1218406
- PRIDE:   P0AGJ6
- EnsemblBacteria:   EBESCT00000029082
- EnsemblBacteria:   EBESCT00000059981
- GeneID:   914879
- GeneID:   957978
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3865
- KEGG:   ecs:ECs3447
- GeneTree:   EBGT00050000008907
- HOGENOM:   HBG714057
- OMA:   HRDRKWE
- ProtClustDB:   PRK10864
- BioCyc:   ECOL83334:ECS3447-MONOMER
- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479
- PIRSF:   PIRSF006280
- SMART:   SM00967

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 37785; Mature: 37785

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796