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The map label for this gene is gcd

Identifier: 170683523

GI number: 170683523

Start: 148888

End: 151278

Strand: Reverse

Name: gcd

Synonym: EcSMS35_0134

Alternate gene names: 170683523

Gene position: 151278-148888 (Counterclockwise)

Preceding gene: 170680677

Following gene: 170680607

Centisome position: 2.98

GC content: 55.37

Gene sequence:

>2391_bases
ATGGCAATTAACAATACAGGCTCGCGACGATTACTCGTCACGCTAACAGCCCTTTTTGCAGCGCTTTGCGGGCTGTATCT
ACTCATTGGCGGAGGCTGGCTGGTCGCGATTGGCGGCTCCTGGTACTACCCTATCGCTGGCCTTGTGATGCTCGGCGTCG
CCTGGATGCTGTGGCGCAGTAAACGCGCCGCGCTTTGGCTGTACGCCGCCCTGCTGCTCGGCACCATGATTTGGGGCGTC
TGGGAAGTTGGTTTCGACTTCTGGGCGCTGACTCCGCGCAGCGACATTCTGGTCTTCTTCGGCATCTGGCTGATCCTGCC
GTTTGTCTGGCGTCGCCTGGTCATTCCTGCCAGCGGCGCAGTTGCCGCACTGGTGGTGGCATTGCTGATTAGTGGCGGAA
TCCTCACCTGGGCGGGATTTAACGATCCGCAGGAAATCAACGGCACCTTAAGCGCCGATGCCACACCTGCCGAAGCTATC
TCACCGGTTGCCGATCAGGACTGGCCTGCGTATGGTCGCAATCAGGAAGGTCAACGCTTTTCACCACTGAAGCAAATTAA
CGCCGATAACGTTCACAACCTGAAAGAAGCCTGGGTGTTCCGTACAGGCGATGTGAAACAGCCGAACGATCCGGGCGAAA
TCACCAATGAAGTGACGCCGATTAAAGTGGGCGATACGCTTTACTTATGTACCGCTCACCAGCGCCTGTTTGCGCTCGAT
GCCGCCAGCGGCAAAGAGAAATGGCATTACGATCCTGAACTGAAAACCAACGAGTCCTTCCAGCACGTAACCTGTCGTGG
CGTCTCTTATCATGAAGCCAAAGCAGAAACGGCCTCGCCAGAAGTGATGGCGGATTGCCCGCGTCGTATCATTCTTCCGG
TCAATGATGGTCGCCTGATTGCGATTAACGCTGAAAACGGCAAGCTGTGCGAAACCTTCGCTAATAAAGGCGTGCTCAAT
CTGCAAAGCAATATGCCGGACACCAAACCGGGTCTGTATGAGCCGACTTCGCCGCCAATTATCACCGATAAAACCATCGT
GATGGCCGGTTCAGTCACCGATAACTTCTCAACCCGCGAAACGTCTGGCGTGATCCGTGGTTTTGATGTCAACACCGGGG
AGCTGCTGTGGGCTTTTGATCCGGGCGCGAAAGATCCGAACGCAATCCCGTCTGACGAACACACCTTTACCTTTAACTCA
CCAAACTCGTGGGCACCAGCGGCCTATGACGCGAAGCTGGATCTGGTGTATCTGCCGATGGGCGTGACCACGCCGGATAT
CTGGGGCGGTAACCGTACACCGGAACAGGAACGTTATGCCAGCTCGATTCTGGCGCTGAATGCCACTACCGGGAAACTGG
CGTGGAGCTACCAGACCGTTCACCACGACCTGTGGGATATGGATCTTCCGGCACAGCCGACGCTGGCGGACATCACCGTT
AATGGTCAGAAAGTGCCAGTCATTTATGCTCCGGCGAAAACCGGCAACATTTTTGTGCTCGATCGTCGTAATGGCGAACT
GGTGGTTCCGGCACCGGAAAAACCGGTTCCCCAAGGTGCTGCCAAAGGCGATTACGTAACCCCAACTCAACCGTTTTCTG
AACTGAGCTTCCGTCCGACGAAAGATTTGAGCGGTGCGGATATGTGGGGAGCCACCATGTTTGACCAGCTGGTGTGCCGC
GTGATGTTCCACCAGATGCGCTATGAAGGCATTTTCACCCCGCCATCTGAACAGGGTACGCTGGTCTTCCCGGGTAACCT
GGGGATGTTCGAATGGGGCGGGATTTCCGTTGATCCGAATCGTGAAGTGGCGATTGCCAACCCAATGGCACTGCCGTTTG
TTTCGAAACTGATCCCACGCGGTCCTGGTAACCCGATGGAACAGCCGAAAGATGCCAAAGGCACGGGTACGGAATCCGGC
ATTCAGCCACAGTACGGTGTACCGTATGGTGTCACGCTCAACCCGTTCCTCTCACCGTTTGGTCTGCCATGTAAACAGCC
AGCATGGGGCTATATCTCGGCATTGGATCTGAAAACCAATGAAGTGGTGTGGAAGAAACGTATTGGTACGCCGCAGGACA
GTATGCCATTCCCGATGCCTGTACCGGTGCCGTTCAATATGGGTATGCCGATGCTGGGCGGGCCAATCTCCACAGCGGGT
AACGTACTGTTTATCGCCGCTACGGCAGATAACTACCTGCGCGCTTACAACATGAGCAACGGTGAAAAACTGTGGCAGGG
CCGTTTACCAGCGGGTGGTCAGGCTACGCCAATGACCTATGAAGTGAATGGCAAGCAGTATGTGGTGATCTCCGCAGGCG
GTCACGGTTCATTTGGTACGAAGATGGGCGACTATATTGTGGCTTATGCGCTGCCGGATGATGTGAAGTAA

Upstream 100 bases:

>100_bases
GAATCTGTTAATAAAGCTCGAATTGTGATGACGATCACACATGTTAAAACCTATAATTAATTGCTACTAAAATATTAATG
AATTGAAATGGTGTCTCTTT

Downstream 100 bases:

>100_bases
CCATAATGCCCGGAGAGATCCGGGCATATTTCCGAATACGGTCTTTTTATACCGTAAACCCTAACATCATCCCCGTATCT
TCATGCTCCAGCAGATGGCA

Product: quinoprotein glucose dehydrogenase

Products: NA

Alternate protein names: Glucose dehydrogenase [pyrroloquinoline-quinone]

Number of amino acids: Translated: 796; Mature: 795

Protein sequence:

>796_residues
MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGV
WEVGFDFWALTPRSDILVFFGIWLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTPIKVGDTLYLCTAHQRLFALD
AASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLN
LQSNMPDTKPGLYEPTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEHTFTFNS
PNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATTGKLAWSYQTVHHDLWDMDLPAQPTLADITV
NGQKVPVIYAPAKTGNIFVLDRRNGELVVPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCR
VMFHQMRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGNPMEQPKDAKGTGTESG
IQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEVVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAG
NVLFIAATADNYLRAYNMSNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK

Sequences:

>Translated_796_residues
MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGV
WEVGFDFWALTPRSDILVFFGIWLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTPIKVGDTLYLCTAHQRLFALD
AASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLN
LQSNMPDTKPGLYEPTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEHTFTFNS
PNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATTGKLAWSYQTVHHDLWDMDLPAQPTLADITV
NGQKVPVIYAPAKTGNIFVLDRRNGELVVPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCR
VMFHQMRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGNPMEQPKDAKGTGTESG
IQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEVVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAG
NVLFIAATADNYLRAYNMSNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK
>Mature_795_residues
AINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVW
EVGFDFWALTPRSDILVFFGIWLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAIS
PVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTPIKVGDTLYLCTAHQRLFALDA
ASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNL
QSNMPDTKPGLYEPTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEHTFTFNSP
NSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATTGKLAWSYQTVHHDLWDMDLPAQPTLADITVN
GQKVPVIYAPAKTGNIFVLDRRNGELVVPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRV
MFHQMRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGNPMEQPKDAKGTGTESGI
QPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEVVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGN
VLFIAATADNYLRAYNMSNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK

Specific function: GDH is probably involved in energy conservation rather than in sugar metabolism

COG id: COG4993

COG function: function code G; Glucose dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein; Periplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial PQQ dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1786316, Length=796, Percent_Identity=100, Blast_Score=1631, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHG_ECOLI (P15877)

Other databases:

- EMBL:   X51323
- EMBL:   D12651
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64735
- RefSeq:   AP_000785.1
- RefSeq:   NP_414666.1
- ProteinModelPortal:   P15877
- SMR:   P15877
- DIP:   DIP-9747N
- IntAct:   P15877
- MINT:   MINT-1267882
- STRING:   P15877
- EnsemblBacteria:   EBESCT00000002761
- EnsemblBacteria:   EBESCT00000002762
- EnsemblBacteria:   EBESCT00000015725
- GeneID:   944830
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0120
- KEGG:   eco:b0124
- EchoBASE:   EB0364
- EcoGene:   EG10369
- eggNOG:   COG4993
- GeneTree:   EBGT00050000009769
- HOGENOM:   HBG586154
- OMA:   LTGADMW
- ProtClustDB:   CLSK879560
- BioCyc:   EcoCyc:GLUCDEHYDROG-MONOMER
- BioCyc:   MetaCyc:GLUCDEHYDROG-MONOMER
- BRENDA:   1.1.5.2
- Genevestigator:   P15877
- InterPro:   IPR017511
- InterPro:   IPR019556
- InterPro:   IPR019551
- InterPro:   IPR018391
- InterPro:   IPR002372
- InterPro:   IPR011047
- InterPro:   IPR001479
- Gene3D:   G3DSA:2.140.10.10
- SMART:   SM00564
- TIGRFAMs:   TIGR03074

Pfam domain/function: PF01011 PQQ; PF10535 PQQ_C; PF10527 PQQ_N; SSF50998 Quin_alc_DH_like

EC number: =1.1.5.2

Molecular weight: Translated: 86748; Mature: 86617

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00363 BACTERIAL_PQQ_1; PS00364 BACTERIAL_PQQ_2

Important sites: ACT_SITE 466-466

Signals:

None

Transmembrane regions:

HASH(0x16c7e174)-; HASH(0x16ade458)-; HASH(0x16aa2f60)-; HASH(0x16069990)-; HASH(0x15839858)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIAGLVMLGVAWMLWRS
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHC
KRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGIWLILPFVWRRLVIPASGA
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
VAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAISPVADQDWPAYGRNQEGQRF
HHHHHHHHHHCCCEEEECCCCCHHHCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCC
SPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTPIKVGDTLYLCTAHQRLFALD
CHHHHCCCCCHHHHHHHEEEECCCCCCCCCCCHHCCCCCEEEECCEEEEEECCCEEEEEE
AASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETASPEVMADCPRRIILPVNDGRLI
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCCHHHHCCCCEEEEEECCCEEE
AINAENGKLCETFANKGVLNLQSNMPDTKPGLYEPTSPPIITDKTIVMAGSVTDNFSTRE
EEECCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCC
TSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEHTFTFNSPNSWAPAAYDAKLDLVYLPM
CCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCEECCEEEEEEEEC
GVTTPDIWGGNRTPEQERYASSILALNATTGKLAWSYQTVHHDLWDMDLPAQPTLADITV
CCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCEEEEEEHHHHHHHCCCCCCCCCEEEEEE
NGQKVPVIYAPAKTGNIFVLDRRNGELVVPAPEKPVPQGAAKGDYVTPTQPFSELSFRPT
CCCEEEEEEECCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCC
KDLSGADMWGATMFDQLVCRVMFHQMRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEECCCCCEEECCCEEECCC
REVAIANPMALPFVSKLIPRGPGNPMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPF
CCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLPCKQPAWGYISALDLKTNEVVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAG
CCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NVLFIAATADNYLRAYNMSNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGT
CEEEEEECCCCEEEEECCCCCHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCC
KMGDYIVAYALPDDVK
CCCCEEEEEECCCCCH
>Mature Secondary Structure 
AINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIAGLVMLGVAWMLWRS
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHC
KRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGIWLILPFVWRRLVIPASGA
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
VAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAISPVADQDWPAYGRNQEGQRF
HHHHHHHHHHCCCEEEECCCCCHHHCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCC
SPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTPIKVGDTLYLCTAHQRLFALD
CHHHHCCCCCHHHHHHHEEEECCCCCCCCCCCHHCCCCCEEEECCEEEEEECCCEEEEEE
AASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETASPEVMADCPRRIILPVNDGRLI
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCCHHHHCCCCEEEEEECCCEEE
AINAENGKLCETFANKGVLNLQSNMPDTKPGLYEPTSPPIITDKTIVMAGSVTDNFSTRE
EEECCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCC
TSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEHTFTFNSPNSWAPAAYDAKLDLVYLPM
CCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCEECCEEEEEEEEC
GVTTPDIWGGNRTPEQERYASSILALNATTGKLAWSYQTVHHDLWDMDLPAQPTLADITV
CCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCEEEEEEHHHHHHHCCCCCCCCCEEEEEE
NGQKVPVIYAPAKTGNIFVLDRRNGELVVPAPEKPVPQGAAKGDYVTPTQPFSELSFRPT
CCCEEEEEEECCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCC
KDLSGADMWGATMFDQLVCRVMFHQMRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEECCCCCEEECCCEEECCC
REVAIANPMALPFVSKLIPRGPGNPMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPF
CCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLPCKQPAWGYISALDLKTNEVVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAG
CCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NVLFIAATADNYLRAYNMSNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGT
CEEEEEECCCCEEEEECCCCCHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCC
KMGDYIVAYALPDDVK
CCCCEEEEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2228962; 8419307; 8202364; 9278503; 8509415; 8554505