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The map label for this gene is potE [H]

Identifier: 170683462

GI number: 170683462

Start: 716182

End: 717501

Strand: Reverse

Name: potE [H]

Synonym: EcSMS35_0714

Alternate gene names: 170683462

Gene position: 717501-716182 (Counterclockwise)

Preceding gene: 170680662

Following gene: 170681346

Centisome position: 14.16

GC content: 50.98

Gene sequence:

>1320_bases
ATGAGTCAGACTAAATCGAACAAGATGGGCGTCGTTCAGTTAACCATACTGACGATGGTCAACATGATGGGCTCCGGTAT
CATCATGCTGCCGACAAAGCTTGCCGAAGTTGGGACAATCTCAATTATCTCCTGGCTGGTGACAGCCGTAGGCTCAATGG
CACTGGCGTGGGCATTCGCAAAATGCGGTATGTTCAGCCGTAAGGCAGGTGGTATGGGCGGTTATGCGGAGTATGCTTTC
GGTAAATCCGGTAACTTTATGGCGAACTACACCTACGGTGTCTCGCTGCTGATTGCTAACGTCGCGATTGCTATTTCGGC
GGTTGGTTACGGCACCGAACTGCTCGGCGCGAGTTTGTCGCCAGTGCAGATTGGTCTTGCGACCATCGGGGTGCTGTGGA
TTTGTACCGTGGCTAACTTCGGTGGTGCGCGCATTACCGGGCAAATCAGTAGCATTACTGTGTGGGGGGTAATCATTCCG
GTCGTCGGTCTGTGCATTATCGGCTGGTTCTGGTTTAGCCCGACGCTGTACGCTAACTCATGGAACCCTCATCATGTACC
GTTCTTTAGCGCTGTAGGTTCCTCCATCGCCATGACGTTGTGGGCTTTCCTCGGTCTGGAGTCTGCCTGTGCCAACGCCG
AAGTAGTGGAAAACCCGGAACGTAACGTGCCAATAGCAGTCCTTGGCGGTACGTTAGGTGCGGCAGTGATTTATATCGTC
TCCACCAACGTGATTGCCGGGATTGTGCCCAATATGGAGCTGGCAAATTCAACGGCACCATTTGGCCTGGCCTTCGCACA
GATGTTCACACCAGAAGTGGGTAAAGTCATTATGGCGCTGATGGTGATGTCCTGCTGCGGTTCACTGCTTGGCTGGCAGT
TCACCATTGCCCAGGTGTTTAAATCTTCATCTGATGAAGGCTACTTCCCTAAAATTTTCTCCCGTGTAACCAAAGTGGAT
GCACCGGTTCAGGGAATGTTGATCATTGTGATTATTCAGAGTGGATTGGCACTGATGACCATTAGCCCGTCACTGAACAG
CCAGTTCAACGTGCTGGTTAACCTGGCCGTGGTGACCAATATCATCCCGTATATTCTGTCGATGGCAGCATTAGTTATTA
TTCAGCAGGTCGCCAATGTGCCACCGTCAAAAGCGAAAGTAGCAAACTTTGTTGCCTTTGTTGGTGCGATGTACAGTTTC
TATGCGCTGTACTCATCCGGGGAAGAAGCCATGCTGTACGGTTCCATCGTGACCTTCCTTGGTTGGACACTGTATGGTCT
GGTATCTCCACGCTTTGAACTGAAAAATAAACACGGTTAA

Upstream 100 bases:

>100_bases
AGGGTGTCTATATCGAAGAACATGATGGTCGTAAGCAAGTTTGGTGCTATGTCATCAAGCCTCGTGGTGCGCAAAGCACC
CTGCTGAAAGGGGAAAAATT

Downstream 100 bases:

>100_bases
AAAAAGCGAAATAAAAAAGGGCGATTGAATGATCGCCCTTTCTGTTTGTTGACAAAGAGTTCGTTATTTATGCCGCATCC
GGCAGGTGTAGGGTCCAAAC

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSQTKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKAGGMGGYAEYAF
GKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIV
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
APVQGMLIIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQQVANVPPSKAKVANFVAFVGAMYSF
YALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG

Sequences:

>Translated_439_residues
MSQTKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKAGGMGGYAEYAF
GKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIV
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
APVQGMLIIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQQVANVPPSKAKVANFVAFVGAMYSF
YALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG
>Mature_438_residues
SQTKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKAGGMGGYAEYAFG
KSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIPV
VGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIVS
TNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDA
PVQGMLIIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQQVANVPPSKAKVANFVAFVGAMYSFY
ALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=439, Percent_Identity=97.9498861047836, Blast_Score=859, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=437, Percent_Identity=32.9519450800915, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1790575, Length=414, Percent_Identity=31.6425120772947, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787890, Length=422, Percent_Identity=26.7772511848341, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540018, Length=338, Percent_Identity=24.8520710059172, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46504; Mature: 46373

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFA
CCCCCCCCCCCEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCGMFSRKAGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLS
HHCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYANS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
WNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCHHHHHHHHHH
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVF
HHHHHHHCCCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSSSDEGYFPKIFSRVTKVDAPVQGMLIIVIIQSGLALMTISPSLNSQFNVLVNLAVVTN
HCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
IIPYILSMAALVIIQQVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GWTLYGLVSPRFELKNKHG
HHHHHHHCCCCHHCCCCCC
>Mature Secondary Structure 
SQTKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFA
CCCCCCCCCCEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCGMFSRKAGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLS
HHCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYANS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
WNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCHHHHHHHHHH
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVF
HHHHHHHCCCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSSSDEGYFPKIFSRVTKVDAPVQGMLIIVIIQSGLALMTISPSLNSQFNVLVNLAVVTN
HCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
IIPYILSMAALVIIQQVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GWTLYGLVSPRFELKNKHG
HHHHHHHCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]