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The map label for this gene is rhmD [H]

Identifier: 170683394

GI number: 170683394

Start: 2449589

End: 2450794

Strand: Reverse

Name: rhmD [H]

Synonym: EcSMS35_2401

Alternate gene names: 170683394

Gene position: 2450794-2449589 (Counterclockwise)

Preceding gene: 170683760

Following gene: 170682702

Centisome position: 48.35

GC content: 52.16

Gene sequence:

>1206_bases
ATGACCCTACCAAAAATCAAACAGGTTCGCGCCTGGTTTACTGGCGGTGCGACAGCAGAAAAAGGCGCTGGCGGTGGTGA
TTATCACGACCAGGGGGCGAATCACTGGATTGACGATCATATTGCCACCCCGATGAGTAAATACCGCGATTACGAGCAGT
CACGCCAGTCATTTGGCATTAACGTTCTTGGCACGTTGATTGTTGAAGTCGAAGCAGAAAACGGCCAGACCGGATTCGCC
GTTTCGACAGCCGGTGAAATGGGCTGTTTTATTGTCGAAAAACACCTTAACCGTTTCATTGAGGGTAAATGTGTCAGTGA
TATCAAACTGATCCACGATCAAATGCTCAATGCCACCCTGTATTACTCCGGCTCTGGTGGCCTGGTGATGAATACGATTT
CCTGTGTCGATCTGGCTCTGTGGGATCTGTTCGGCAAAGTGGTCGGGCTTCCGGTTTATAAACTTTTAGGCGGCGCAGTT
CGTGATGAGATTCAGTTCTACGCCACAGGTGCGCGTCCGGATCTGGCAAAAGAGATGGGCTTTATCGGTGGCAAAATGCC
GACGCACTGGGGGCCACATGATGGCGATGCGGGGATCCGCAAAGATGCCGCTATGGTCGCGGATATGCGTGAAAAATGCG
GTGAGGATTTCTGGTTAATGCTCGACTGCTGGATGAGTCAGGACGTGAACTATGCCACCAAACTGGCCCACGCTTGCGCG
CCCTATAACCTGAAATGGATCGAAGAGTGCCTGCCGCCACAGCAGTATGAAGGTTATCGCGAACTGAAACGCAACGCACC
AGCGGGGATGATGGTCACCAGCGGTGAGCACCACGGCACATTGCAATCTTTCCGTACGCTTTCAGAAACCGGTATCGACA
TTATGCAGCCGGATGTTGGCTGGTGCGGTGGCTTAACCACGCTGGTGGAAATTGCCGCAATCGCCAAATCCAGGGGGCAA
CTGGTGGTGCCGCACGGTTCGTCTGTTTACTCCCACCATGCGGTGATCACCTTCACCAATACGCCATTCAGTGAATTCCT
GATGACCAGCCCGGATTGTTCAACGATGCGTCCGCAATTTGATCCGATTCTGCTTAATGAGCCGGTTCCGGTGAATGGTC
GTATTCATAAATCAGTGCTTGATAAACCCGGTTTCGGCGTTGAACTCAATCGTGACTGCAATTTGAAACGCCCCTACAGC
CACTAA

Upstream 100 bases:

>100_bases
ATCTGGCGGGCAAGGCCATTGCCTGCGCCAGGGATATTTCACGTCTGTTGGGATGGAAAAGTCCCTTTGACTTACAGGCC
TCATAACAATGGAGAACAGC

Downstream 100 bases:

>100_bases
TCACTCCGGGCGTTGCTGTGACAACGGTAACGCCCACATATTCTAAGGAGCTTTTTATGAGCACCGCTTTGCTTGACGCC
GTGGTGAAGAAAAACCGCGC

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFA
VSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACA
PYNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQ
LVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYS
H

Sequences:

>Translated_401_residues
MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFA
VSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACA
PYNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQ
LVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYS
H
>Mature_400_residues
TLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAV
STAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVR
DEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACAP
YNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQL
VVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=401, Percent_Identity=99.002493765586, Blast_Score=835, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=243, Percent_Identity=30.8641975308642, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44210; Mature: 44079

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGI
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
NVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATL
EEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEE
YYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAKEMG
EECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHHHHHH
FIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACA
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHC
PYNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVG
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEECCCCC
WCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQF
HHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCCCCCC
DPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH
CCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
TLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGI
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
NVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATL
EEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEE
YYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAKEMG
EECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHHHHHH
FIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACA
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHC
PYNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVG
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEECCCCC
WCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQF
HHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCCCCCC
DPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH
CCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA