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The map label for this gene is dcuA [H]

Identifier: 170683393

GI number: 170683393

Start: 1104532

End: 1105830

Strand: Reverse

Name: dcuA [H]

Synonym: EcSMS35_1089

Alternate gene names: 170683393

Gene position: 1105830-1104532 (Counterclockwise)

Preceding gene: 170683881

Following gene: 170682542

Centisome position: 21.82

GC content: 45.96

Gene sequence:

>1299_bases
ATGGTTTGGCTGGAAATTATCGTTGTACTTGGCGCAATATTTTTTGGTATTCGTCAGGGAGGAATCGGCATTGGTTTATG
CGGCGGTCTTGGCCTTGCGATCCTGACTCTGGGATTTGGTCTGCCTATGGGATCACCGCCAGTCGATGTGATCCTGATTA
TTATGACCGTAGTTGTGGCAGCTTCAGCCTTACAGGCGGCAGGTGGGATGGATTATCTGGTACGTTTAGCCAGTAATTTC
ATGCGACGTAACCCAAAATATATCAACATAATCGCACCGATTATCACTTGGTTAATGACCATTATGGCCGGTACCGGGTT
TATTGTTTTCTCCACACTGCCGGTAATTGCTGAAGTAGCAAAAGAGTCCGGTATCCGGCCTTCCCGCACACTCGCTGGCT
CAGTTGTCGCCTCACAGGTTGCAATTTCTGGCTCACCAATCAGTGCCGCAATGGCAGCTATGCTCACTATTATGGAGGTT
AATGGCGTCAGTTTTATTCAGGTGATGTCGGTATGCCTTCCCACCTCTTTTGTCGCCGCGATGGTTGCTGCTTTTATCGC
CTCCCGACAGGGGTGTGAATTACAAGATGACGAAGTATATCTTGAACGTCTGCAAAAAGGTCTGGTGCAAAAATACGAGA
ATAATAACAGCATCAAACCTGGTGCAACGCTTTCCGTCGGATTATTCATGCTGGCGACGATCGCCATTGTTATTCTTGCT
GCATTCCCCCAATTGCGTCCGGGTTTTGATATCAGCAAACCGATGGAAACGCGCGATATTATTATTATTTGCATGCTCTC
TGCTGCCTGCCTGATGGTAATACTGTGTAAGATGTCTACTGATGACATTATTCTGACCTCCACATTTCGTGCTGGAATGA
GTTCGCTGGCAGTGATTCTTGGTATTGTAACCTTAGGTACCACCTTTATTGATGCGCATTTAACTGAGATCAAAGATATC
GCTGGTGATATTTTACAAACTTATCCGATGCTTTTAGCATTAGTTCTTTTTTTTACCTGCGCTTTACTTTACTCACAGGG
AGCGACTACGCCGTTAATTATTCCTCTGGCTGTAGCACTCAACATACCAACCTGGGCAATTCTTGCATCTTATGTTGCCG
TTACGGGAGTATTCGTGCTTCCAACGTATCCAACATCACTCGCCGCGATGGAATTTGATACTACCGGCACAACCCGTGTG
GGCAATTATTTATTAAATCACCCATTTATGCTACCCGGGTTGGGTGGTGTTATTGCAGGAGTGACATTTGGGTTTGTGAT
TGCACCAATGATGGTCTGA

Upstream 100 bases:

>100_bases
TATGAAAATCGTATCGGAAAAGATCAACATTTAACTCAATAATTCTAAATCACAGCCAGAAAGTTATTTTACAAAGATTA
TATTTACAACGGAGAACATC

Downstream 100 bases:

>100_bases
TAGAATCATAATTTTCATTCTTTATGGAAGTAGTTACAGAGTTACTATTTGATAATTAGTCAGGCAGTTAATCTGCATTC
AGTTACTGACTGTCAGAAGA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMGSPPVDVILIIMTVVVAASALQAAGGMDYLVRLASNF
MRRNPKYINIIAPIITWLMTIMAGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
NGVSFIQVMSVCLPTSFVAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNNSIKPGATLSVGLFMLATIAIVILA
AFPQLRPGFDISKPMETRDIIIICMLSAACLMVILCKMSTDDIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDI
AGDILQTYPMLLALVLFFTCALLYSQGATTPLIIPLAVALNIPTWAILASYVAVTGVFVLPTYPTSLAAMEFDTTGTTRV
GNYLLNHPFMLPGLGGVIAGVTFGFVIAPMMV

Sequences:

>Translated_432_residues
MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMGSPPVDVILIIMTVVVAASALQAAGGMDYLVRLASNF
MRRNPKYINIIAPIITWLMTIMAGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
NGVSFIQVMSVCLPTSFVAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNNSIKPGATLSVGLFMLATIAIVILA
AFPQLRPGFDISKPMETRDIIIICMLSAACLMVILCKMSTDDIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDI
AGDILQTYPMLLALVLFFTCALLYSQGATTPLIIPLAVALNIPTWAILASYVAVTGVFVLPTYPTSLAAMEFDTTGTTRV
GNYLLNHPFMLPGLGGVIAGVTFGFVIAPMMV
>Mature_432_residues
MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMGSPPVDVILIIMTVVVAASALQAAGGMDYLVRLASNF
MRRNPKYINIIAPIITWLMTIMAGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
NGVSFIQVMSVCLPTSFVAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNNSIKPGATLSVGLFMLATIAIVILA
AFPQLRPGFDISKPMETRDIIIICMLSAACLMVILCKMSTDDIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDI
AGDILQTYPMLLALVLFFTCALLYSQGATTPLIIPLAVALNIPTWAILASYVAVTGVFVLPTYPTSLAAMEFDTTGTTRV
GNYLLNHPFMLPGLGGVIAGVTFGFVIAPMMV

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=435, Percent_Identity=48.5057471264368, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790565, Length=404, Percent_Identity=33.6633663366337, Blast_Score=244, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45615; Mature: 45615

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]