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The map label for this gene is hemD [H]

Identifier: 170683392

GI number: 170683392

Start: 4268731

End: 4269471

Strand: Reverse

Name: hemD [H]

Synonym: EcSMS35_4169

Alternate gene names: 170683392

Gene position: 4269471-4268731 (Counterclockwise)

Preceding gene: 170680193

Following gene: 170682020

Centisome position: 84.24

GC content: 53.17

Gene sequence:

>741_bases
ATGAGTATCCTGGTCACCCGCCCGTCTCCCGCTGGAGAAGAGTTAGTGAGCCGTCTGCGCACACTGGGGCAGGTGGCCTG
GCATTTTCCGCTGATTGAGTTTTCTCCGGGTCGACAATTACCGCAACTTGCTGATCAACTGGCAGCGCTGGGGGAGAGCG
ATCTGTTGTTTGCCCTCTCGCAACACGCGGTTGCTTTTGCCCAATCACAGCTACATCAGCAAGATCGTAAATGGCCCCGA
TTACCTGATTATTTCGCCATTGGACGCACCACCGCACTGGCATTACATACCGTAAGCGGACAGAAGATTCTCTACCCGCA
GGATCGGGAAATCAGCGAAGTCTTGCTACAATTACCTGAATTACAAAATATTGCGGGCAAACGTGCGCTGATATTACGTG
GCAATGGCGGTCGTGAGCTAATTGGGGATTCCCTGACGGCGCGCGGTGCTGAGGTCACTTTTTGTGAATGTTATCAACGA
TGCGCAATCCATTACGATGGTGCAGAAGAAGCGATGCGCTGGCAATCCCGCGAGGTGACGACGGTCGTTGTTACCAGCGG
TGAAATGTTGCAGCAACTCTGGTCGCTGATCCCACAATGGTATCGTGAGCACTGGTTACTACGCTGTCGACTATTGGTCG
TCAGTGAGCGTTTGGCGAAACTCGCCCGGGAACTGGGCTGGCAAGACATTAAGGTCGCCGATAACGCTGACAACGATGCG
CTTTTACGGGCATTACAATAA

Upstream 100 bases:

>100_bases
CGCAAGATGCCGAACAAATGGGGATTTCGCTGGCAGAAGAGCTACTGAATAACGGCGCGCGCGAGATCCTCGCTGAAGTC
TATAACGGAGACGCCCCGGC

Downstream 100 bases:

>100_bases
CTCTCATAACAGGAAGCCATAATGACGGAACAAGAAAAAACCTCCGCCGTGGTTGAAGAGACCAGGGAGGCCGTGGACAC
CACGTCACAACCTGTCGCAA

Product: uroporphyrinogen-III synthase

Products: NA

Alternate protein names: UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLADQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPR
LPDYFAIGRTTALALHTVSGQKILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQR
CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERLAKLARELGWQDIKVADNADNDA
LLRALQ

Sequences:

>Translated_246_residues
MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLADQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPR
LPDYFAIGRTTALALHTVSGQKILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQR
CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERLAKLARELGWQDIKVADNADNDA
LLRALQ
>Mature_245_residues
SILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLADQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRL
PDYFAIGRTTALALHTVSGQKILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQRC
AIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERLAKLARELGWQDIKVADNADNDAL
LRALQ

Specific function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen-III synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790236, Length=246, Percent_Identity=97.9674796747967, Blast_Score=491, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003754 [H]

Pfam domain/function: PF02602 HEM4 [H]

EC number: =4.2.1.75 [H]

Molecular weight: Translated: 27817; Mature: 27686

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLADQLAALGESDLLFALS
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
QHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQKILYPQDREISEVLLQLPE
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHEEEEEECCCCEEECCCCCCHHHHHHHCHH
LQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQRCAIHYDGAEEAMRWQSREVT
HHHCCCCEEEEEECCCCHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCEE
TVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERLAKLARELGWQDIKVADNADNDA
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHH
LLRALQ
HHHHCC
>Mature Secondary Structure 
SILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLADQLAALGESDLLFALS
EEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
QHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQKILYPQDREISEVLLQLPE
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHEEEEEECCCCEEECCCCCCHHHHHHHCHH
LQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQRCAIHYDGAEEAMRWQSREVT
HHHCCCCEEEEEECCCCHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCEE
TVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERLAKLARELGWQDIKVADNADNDA
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHH
LLRALQ
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3054815; 3320969; 3277628; 3040684; 1379743; 9278503 [H]