Click here to switch to the map view.

The map label for this gene is tmcA [H]

Identifier: 170683380

GI number: 170683380

Start: 2678124

End: 2680139

Strand: Reverse

Name: tmcA [H]

Synonym: EcSMS35_2621

Alternate gene names: 170683380

Gene position: 2680139-2678124 (Counterclockwise)

Preceding gene: 170683921

Following gene: 170683913

Centisome position: 52.88

GC content: 55.31

Gene sequence:

>2016_bases
ATGGCTGAACTGACTGCGCTTCACACATTAACAGCGCAAATGAAACGTGAAGGGATCCGCCGCTTGCTGGTGTTGAGCGG
GGAAGAGGGTTGGTGTTTTGATCATGCGCTTAAGTTGCGTGATGCCTTACCTGGCGACTGGCTGTGGATTTCGCCGCAGC
CAGATGCTGAAAACCACTGTTCTCCCTCGGCACTACAAACTTTACTTGGGCGCGAGTTCCGGCATGCGGTATTCGACGCC
CGCCACGGCTTTGATGCCGCTGCCTTTGCGGCACTTAGCGGAACGTTGAAAGCGGGAAGCTGGCTAGTGTTGTTACTCCC
TGTATGGGATGAGTGGGAAAACCAACCTGATGCCGACTCGCTGCGCTGGAGTGATTGCCCTGACCCTATTGCGACGCCGC
ATTTTGTCCAGCATTTCAAACGCGTACTTACGGCGAATAACGACGCTATCCTCTGGCGGCAAAACCAGCCGTTCTCGTTG
GCGCATTTTACTCCCCGTACTGACTGGCACCCCGCGACCGGCGCACCACAGCCAGAACAACAGCAACTCTTACAGCAGCT
ACTGACCATGCCATTGGGCGTGGCGGTGGTAACGGCTGCGCGTGGGCGCGGTAAATCGGCGCTGGCAGGGCAACTCATTT
CTCGTATTGCGGGTAGTGCGATTGTCACCGCGCCCGCAAAAGCGGCAACGTATGTACTGGCACAATTTGCGGGCGAGAAG
TTTCGCTTTATTGCACCGGATGCCTTGTTAGCCAGCGATGAGCAAGCCGACTGGCTGGTGGTCGATGAAGCCGCAGCCAT
ACCTGCGCCGTTGTTGCATCAACTGGTATCGCGTTTTCCTCGAACGTTGTTAACCACTACGGTGCAGGGCTACGAAGGCA
CCGGACGTGGTTTTTTGCTGAAATTTTGCGCTCGCTTTCCGCATTTACACCGTTTTGAACTGCAACAACCGATCCGCTGG
GCGCAGGGATGCCCGCTGGAGAAAATGGTCAGCGAGGCACTGGTGTTTGACGATGAAAACTTCACTCACGAACCACAAGG
TGACATCGTCATTTCTGCTTTTGAACAGACGTTATGGCGAAGCGAACCAGAAACGCCGTTAAAGGTATATCAGCTATTGT
CTGGCGCGCACTACCGGACCTCGCCGCTGGATTTACGCCGGATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGCT
GGCGAAAACGAGATTGCCGGAGCGCTGTGGCTGGTGGATGAGGGGGGATTATCTCAACAACTCAGTCAGGCGGTATGGGC
AGGTTTTCGTCGCCCTCGGGGTAATCTGGTGGCCCAGTCGCTGGCGGCGCACGGCAGCAATCCACTGGCAGCGACATTGC
GTGGACGGCGGGTAAGCCGGATAGCAGTTCATCCGGCGCGTCAGCGGGAAGGCACAGGGCGGCAACTTATTGCTGGTGCT
TTGCAATATACGCATGACCTCGACTATCTTTCGGTGAGTTTTGGTTACACCGGGGAGTTATGGCGTTTCTGGCAACGCTG
CGGTTTTGTGCTGGTGCGAATGGGTAATCATCGTGAAGCCAGCAGCGGTTGCTATACGGCGATGGCACTGTTACCGATGA
GTGATGCGGGTAAACAGCTGGCTGAACGTGAGCATTACCGTTTACGTCGCGATGCGCAAGCTCTCGCGCAGTGGAATGGC
GAAATGCTTCCCGTTGATCCACTAAACGATGCCGTCCTTTCTGACGACGACTGGCTTGAACTGGCCGGTTTTGCTTTCGC
TCATCGTCCGCTATTAACGTCGTTAGGTTGCTTAATGCGTCTGTTACAAACCAGCGAAATGGCATTACCGGCGCTGCGTG
GGCGTTTACAGAAAAACGCCAGTGACGCGCAGTTATGTACCACACTTAAACTTTCAGGCCGTAAGCTGTTACTGGTCCGT
CAGCGGGAAGAGGCCGCGCAGGCGCTATACGCACTTGATGATGTTCGCACTGAGCGTTTGCGCGATCGCATAACGCAATG
GCAATTTTTTCACTGA

Upstream 100 bases:

>100_bases
CTTCTCAGCAACGCTACAGCTGGTTTAAACGTGGTTTCGACAGCGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGC
ATTTAACTTCCGGGGCAGGG

Downstream 100 bases:

>100_bases
CTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGACATCAGTTAAGGAGTTCGCCATGAAAC
ATGACCATTTTGTTGTTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHEPQGDIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNG
EMLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQLCTTLKLSGRKLLLVR
QREEAAQALYALDDVRTERLRDRITQWQFFH

Sequences:

>Translated_671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHEPQGDIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNG
EMLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQLCTTLKLSGRKLLLVR
QREEAAQALYALDDVRTERLRDRITQWQFFH
>Mature_670_residues
AELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDAR
HGFDAAAFAALSGTLKAGSWLVLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSLA
HFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEKF
RFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWA
QGCPLEKMVSEALVFDDENFTHEPQGDIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAG
ENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGAL
QYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGE
MLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQLCTTLKLSGRKLLLVRQ
REEAAQALYALDDVRTERLRDRITQWQFFH

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally si

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=671, Percent_Identity=95.8271236959762, Blast_Score=1313, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507621, Length=366, Percent_Identity=26.2295081967213, Blast_Score=86, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324197, Length=384, Percent_Identity=25.78125, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24640662, Length=449, Percent_Identity=25.1670378619154, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 74941; Mature: 74810

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHC
CCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWDEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHCCCCCCCCCC
LRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
QQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
HHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHEPQGDIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEHHHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQQ
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCHHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCEEEEEEECCHHHCCCCCHHHHHHH
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAQALAQWNGEMLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMR
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSEMALPALRGRLQKNASDAQLCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC
>Mature Secondary Structure 
AELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHC
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWDEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHCCCCCCCCCC
LRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
QQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
HHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHEPQGDIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEHHHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQQ
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCHHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCEEEEEEECCHHHCCCCCHHHHHHH
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAQALAQWNGEMLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMR
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSEMALPALRGRLQKNASDAQLCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]