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The map label for this gene is argS [H]

Identifier: 170683284

GI number: 170683284

Start: 1298565

End: 1300298

Strand: Reverse

Name: argS [H]

Synonym: EcSMS35_1310

Alternate gene names: 170683284

Gene position: 1300298-1298565 (Counterclockwise)

Preceding gene: 170683171

Following gene: 170680321

Centisome position: 25.66

GC content: 53.0

Gene sequence:

>1734_bases
GTGAATATTCAGGCTCTTCTCTCAGAAAAAGTCCGTCAGGCCATGATTGCGGCAGGCGCGCCTGCGGATTGCGAACCGCA
GGTTCGTCAGTCAGCAAAAGTTCAGTTCGGCGACTATCAGGCTAACGGCATGATGGCAGTTGCTAAAAAACTGGGTATGG
CACCGCGACAATTAGCAGAGCAGGTGCTGACTCATCTGGATCTTAACGGTATCGCCAGCAAAGTTGAGATCGCGGGTCCT
GGCTTTATCAACATTTTCCTTGATCCGGCATTCCTGGCTGATCATGTTCAGCAGGCGCTGGCGTCCGATCGTCTCGGTGT
TGCTACGCCAGAAAAACAGACCATTGTGGTTGACTACTCTGCGCCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGC
GCTCTACCATTATTGGTGACGCAGCAGTTCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGC
GACTGGGGCACTCAGTTCGGTATGCTGATTGCGTGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGC
TGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCATTACGACGAAGATGAAGAGTTTGCCGAGCGTGCACGTAACTACG
TGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAG
ATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGTGAAAGCCTCTACAACCCAATGCTGCCGGG
TATCGTAGCGGACCTGAAAGCCAAAGGTCTGGCAGTAGAAAGTGAAGGCGCGACCGTCGTATTCCTTGATGAGTTCAAAA
ACAAGGAAGGCGAACCGATGGGCGTGATCATTCAGAAGAAAGATGGTGGCTATCTCTACACCACCACTGATATCGCCTGT
GCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCA
GGCGTGGGCTATCGTCCGCAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGG
GTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCAGACCTGCTGGATGAAGCCCTGGAACGC
GCACGCCGTCTGGTGGCAGAGAAGAACCCGGATATGCCAGCCGATGAGCTGGAAAAACTGGCTAATGCGGTTGGTATTGG
TGCGGTTAAATACGCAGATCTCTCCAAAAACCGTACCACTGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGG
GTAATACCGCGCCATACATGCAGTATGCTTACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAA
CTGGCTGCAGCTCCGGTAATCATCCGTGAAGATCGTGAAGCACAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCT
CACTGTGGTTGCCCGTGAAGGCACGCCGCATGTGATGTGTGCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCT
ACGAGCACTGCCCGATCCTCAGCGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTGAAACTGGCACAACTGACGGCGAAG
ACGCTGAAACTGGGTCTGGATACGCTGGGTATTGAGACAGTAGAGCGTATGTAA

Upstream 100 bases:

>100_bases
ACGTTGCTGACGAAAACAGCCATTTGCAGTATACTCCCGCCCTAATTTCTTTAACTGGTGCGGGCAAATTTTGCTCGCTT
CATCAATGTAAGGTATTCCG

Downstream 100 bases:

>100_bases
TCGCGTTATACGGCAAAAGCCGATGTCATGGCAGACATCGGCTTTTTTATCATTAACGATAATTCACAATCACTTCATTC
CGTTGTACCTTTAGCGGCGG

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGP
GFINIFLDPAFLADHVQQALASDRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIAC
AKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALER
ARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAK
TLKLGLDTLGIETVERM

Sequences:

>Translated_577_residues
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGP
GFINIFLDPAFLADHVQQALASDRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIAC
AKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALER
ARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAK
TLKLGLDTLGIETVERM
>Mature_577_residues
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGP
GFINIFLDPAFLADHVQQALASDRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIAC
AKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALER
ARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAK
TLKLGLDTLGIETVERM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15149476, Length=599, Percent_Identity=38.5642737896494, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI197100773, Length=537, Percent_Identity=29.2364990689013, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1788184, Length=577, Percent_Identity=99.8266897746967, Blast_Score=1193, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985061, Length=600, Percent_Identity=37.3333333333333, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71985068, Length=564, Percent_Identity=37.7659574468085, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17532153, Length=384, Percent_Identity=27.6041666666667, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320548, Length=544, Percent_Identity=34.375, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6321883, Length=538, Percent_Identity=28.8104089219331, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI18859963, Length=601, Percent_Identity=38.1031613976705, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI28571570, Length=478, Percent_Identity=29.2887029288703, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d [H]

EC number: =6.1.1.19 [H]

Molecular weight: Translated: 64670; Mature: 64670

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAE
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEECCCCCCCHHHHHHHHCCCHHHHHH
QVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALASDRLGVATPEKQTIVVDYS
HHHHHCCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
APNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVGDWGTQFGMLIAWLEKQQQEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCC
AGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
CCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHEEECCCE
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPM
EEEEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCE
GVIIQKKDGGYLYTTTDIACAKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPE
EEEEEECCCCEEEEECCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCC
SVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALERARRLVAEKNPDMPADELEKL
CCCHHHHHHHHHCCCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
HHHHCCCCEEEECCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHH
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPIL
HHHCCEEEECCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
SAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAE
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEECCCCCCCHHHHHHHHCCCHHHHHH
QVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALASDRLGVATPEKQTIVVDYS
HHHHHCCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
APNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVGDWGTQFGMLIAWLEKQQQEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCC
AGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
CCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHEEECCCE
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPM
EEEEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCE
GVIIQKKDGGYLYTTTDIACAKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPE
EEEEEECCCCEEEEECCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCC
SVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALERARRLVAEKNPDMPADELEKL
CCCHHHHHHHHHCCCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
HHHHCCCCEEEECCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHH
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPIL
HHHCCEEEECCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
SAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA