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The map label for this gene is gltK [H]

Identifier: 170683230

GI number: 170683230

Start: 685911

End: 686585

Strand: Reverse

Name: gltK [H]

Synonym: EcSMS35_0674

Alternate gene names: 170683230

Gene position: 686585-685911 (Counterclockwise)

Preceding gene: 170681365

Following gene: 170680355

Centisome position: 13.55

GC content: 50.81

Gene sequence:

>675_bases
ATGTACGAGTTTGACTGGAGTTCCATAGTTCCTTCCCTGCCATATCTGCTCGACGGGCTGGTGATCACCCTGAAAATCAC
CGTCACGGCAGTCGTGATTGGTATTTTGTGGGGCACCATGCTGGCGGTGATGCGCTTGTCCAGCTTTGCGCCCGTAGCGT
GGTTTGCCAAAGCCTACGTTAACGTATTCCGCTCGATTCCTTTAGTTATGGTTTTGCTGTGGTTTTACCTGATTGTGCCG
GGTTTTCTGCAAAACGTGCTGGGATTATCGCCAAAAAATGATATCCGTTTAATCTCGGCGATGGTGGCGTTTTCGATGTT
TGAAGCGGCCTACTATTCAGAGATTATCCGTGCCGGTATTCAAAGTATTTCCCGTGGTCAGTCGAGCGCCGCGCTGGCGT
TGGGGATGAATCACTGGCAGTCGATGAAACTGATTATTCTGCCGCAGGCGTTCCGCGCGATGGTGCCGCTGCTGCTCACT
CAGGGCATCGTACTGTTCCAGGATACCTCACTGGTGTATGTGTTATCTCTGGCCGATTTCTTCCGTACCGCCTCAACCAT
TGGTGAGCGTGATGGTACGCAGGTCGAGATGATCCTGTTTGCCGGGTTTGTTTATTTCGTTATTAGCCTTAGCGCATCGT
TGTTGGTCAGCTACTTGAAAAGAAGGACAGCATAA

Upstream 100 bases:

>100_bases
CCATTACGCTTGCGTATGTGTTGATTAACGCGGTTATCATGCTGGTAATGACGCTGGTTGAACGTAAAGTTCGCCTGCCC
GGCAACATGGGGGGCAAATA

Downstream 100 bases:

>100_bases
TGATTACCCTGAAAAATGTTTCAAAATGGTATGGTCACTTTCAGGTGCTGACCGACTGCTCAACCGAAGTGAAAAAAGGC
GAAGTGGTGGTGGTTTGCGG

Product: glutamate/aspartate ABC transporter permease GltK

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMNHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA

Sequences:

>Translated_224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMNHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
>Mature_224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMNHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=99.5535714285714, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=36.9158878504673, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI87082239, Length=208, Percent_Identity=31.7307692307692, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787030, Length=227, Percent_Identity=30.8370044052863, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786874, Length=215, Percent_Identity=25.5813953488372, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787087, Length=217, Percent_Identity=27.1889400921659, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI48994930, Length=130, Percent_Identity=34.6153846153846, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788646, Length=210, Percent_Identity=23.3333333333333, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788645, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24928; Mature: 24928

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYV
CCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMNHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYV
CCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMNHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]