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The map label for this gene is cmoB [H]

Identifier: 170683171

GI number: 170683171

Start: 1305817

End: 1306788

Strand: Reverse

Name: cmoB [H]

Synonym: EcSMS35_1315

Alternate gene names: 170683171

Gene position: 1306788-1305817 (Counterclockwise)

Preceding gene: 170684168

Following gene: 170683284

Centisome position: 25.78

GC content: 51.95

Gene sequence:

>972_bases
ATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTGGAAACGCTGCCCGCGCAGAT
TGCTAACTGGCAGCGCGAACAGCAGCACGGTCTGTTTAAACAGTGGTCCAACGCGGTGGAATTTCTGCCTGAAATTAAAC
CGTATCGTCTGGATTTATTGCATAGCGTAACCGCCGAAAGCGAAGAGCCACTGAGCGCCGGGCAAATTAAACGCATTGAA
ACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTC
CGACTGGAAATGGGATCGCGTTCTGCCCCATCTTTCTGATTTAACCGGGCGCACCATTCTTGATGTCGGCTGCGGCAGCG
GTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGAATCGACCCTACGCAACTGTTTCTGTGCCAG
TTTGAAGCGGTGCGTAAACTACTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGTATTGAACAGCTTCCGGCACT
GAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTATCATCGTCGTTCACCGCTGGAGCATCTCTGGCAGTTGAAAG
ATCAACTGGTGAATGAAGGTGAACTGGTGCTTGAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTGCCGGGC
GATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCTGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTT
TGTTGATATTCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAACAGCGACGCACCGAATGGATGGTTACTGAATCGC
TGGCCGATTTCCTCGACCCGCATGATCCGAGTAAGACAGTTGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCG
CGCAAGCCGTAA

Upstream 100 bases:

>100_bases
ATAAAGCACGCCTGCATAAAGCCGGTTTTGAGCATAGCGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCA
TTAAAAGCAGAGGACGCTGC

Downstream 100 bases:

>100_bases
AGATTAGCGCCCGTTTCCGGGCGCTTTTCACGTCTTATGCCTGAACAGCTGGCTGATCAAACGCCGTTAACTTCGGTGCA
TTGCCCGTATATTTTTCGAT

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIE
TLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIA
RKP

Sequences:

>Translated_323_residues
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIE
TLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIA
RKP
>Mature_323_residues
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIE
TLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIA
RKP

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family [H]

Homologues:

Organism=Escherichia coli, GI1788178, Length=323, Percent_Identity=99.6904024767802, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010017 [H]

Pfam domain/function: PF08003 Methyltransf_9 [H]

EC number: NA

Molecular weight: Translated: 37037; Mature: 37037

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
HSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSD
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
LTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLG
CCCCEEEEECCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECC
IEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECC
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDP
HHHHHHCCEEECCHHHHHHHHHHHCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCC
HDPSKTVEGYPAPKRAVLIARKP
CCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
HSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSD
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
LTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLG
CCCCEEEEECCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECC
IEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECC
DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDP
HHHHHHCCEEECCHHHHHHHHHHHCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCC
HDPSKTVEGYPAPKRAVLIARKP
CCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA