Click here to switch to the map view.

The map label for this gene is araJ [H]

Identifier: 170683106

GI number: 170683106

Start: 433575

End: 434843

Strand: Reverse

Name: araJ [H]

Synonym: EcSMS35_0425

Alternate gene names: 170683106

Gene position: 434843-433575 (Counterclockwise)

Preceding gene: 170682477

Following gene: 170681396

Centisome position: 8.58

GC content: 50.83

Gene sequence:

>1269_bases
TTGGCCTCGCTGGTCGTTATCCTGCAAGCTATCACTTTATTGGCTACGGTGATTAGTAGCCGTTCTGGTGGTTGTGATGG
TGGTATGAAAAAAGTCATTTTATCTTTGGCTCTGGGCACGTTTGGTTTGGGGATGGCCGAATTTGGCATTATGGGCGTGC
TCACGGAGCTGGCGCATAACGTAGGAATTTCGATTCCTGCTGCCGGGCATATGATCTCGTATTATGCGCTGGGGGTGGTG
GTCGGTGCGCCAATCATCGCACTCTTTTCCAGCCGCTACTCACTCAAACATATCTTATTGTTTCTGGTGGCGTTGTGCGT
CATTGGCAACGCCATGTTCACGCTCTCTTCGTCTTACCTGATGCTCGCCATTGGTCGGCTGGTATCCGGCTTTCCGCATG
GCGCATTTTTTGGCGTCGGCGCGATCGTGTTATCAAAAATTATCAAACCCGGAAAAGTCACCGCCGCCGTGGCGGGGATG
GTTTCCGGGATGACAGTCGCCAATTTGCTGGGCATTCCGCTGGGAACGTATTTAAGTCAGGAATTTAGCTGGCGTTACAC
CTTTTTATTGATCGCTGTTTTTAATATTGTGGTGATGGCATCGGTCTATTTTTGGGTGCCGGATATTCGCGACGAAGCGA
AAGGAAAGCTGCGCGAACAATTTCACTTTTTACGCAGCCCGGCCCCGTGGTTAATTTTCGCCGCCACCATGTTTGGCAAC
GCAGGTGTATTTGCCTGGTTCAGCTACGTAAAGCCATACATGATGTTTATTTCCGGTTTTTCGGAAACGGCGATGACCTT
TATTATGATGTTAGTGGGGCTAGGGATGGTGCTGGGGAATGTGCTAAGTGGCCGAATTTCAGGACGTTATTCACCACTGC
GCATTGCAGCAGTGACTGACTTTATCATTGTACTGGCACTGCTGATGCTCTTTTTCTGCGGCGGCATGAAAATAACGTCG
CTTATTTTTGCTTTTATTTGTTGCGCGGGATTATTTGCCCTTTCAGCACCTCTGCAAATATTGTTACTGCAAAACGCCAA
AGGCGGAGAGTTATTAGGTGCCGCAGGTGGGCAAATAGCGTTTAACCTCGGTAGCGCCGTCGGCGCATATTGCGGTGGTA
TGATGCTGACGCTGGGGCTGGCATATAATTACGTGGCGCTGCCTGCCGCCCTGCTTTCGTTTGCTGCGATGTCGTCGTTG
CTGCTGTATGGTCGCTATAAGCGCCAGCAAGCGGCGGATAGTCCGGTGCTGGCGAAACCACTGGGGTAG

Upstream 100 bases:

>100_bases
TGAAAAAGATCAACGGCCTGGGCTACAGCAAACTGGAAAGTGCGTTTGCAGTGAAATAACTATTCAGCAGGATAATGAAT
ACAGAGGGGCGAATTATCTC

Downstream 100 bases:

>100_bases
ATTACTGTCACGTTGCGCGTTGCTTATTGCCGGATGCGGCATGAACGCGTTATCGGCCTGCGTTTCCGGCACATTTTGCA
GGCCTGATAAGACGCGGCAA

Product: MFS transport protein AraJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MASLVVILQAITLLATVISSRSGGCDGGMKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMISYYALGVV
VGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSGFPHGAFFGVGAIVLSKIIKPGKVTAAVAGM
VSGMTVANLLGIPLGTYLSQEFSWRYTFLLIAVFNIVVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGN
AGVFAWFSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCGGMKITS
LIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSL
LLYGRYKRQQAADSPVLAKPLG

Sequences:

>Translated_422_residues
MASLVVILQAITLLATVISSRSGGCDGGMKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMISYYALGVV
VGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSGFPHGAFFGVGAIVLSKIIKPGKVTAAVAGM
VSGMTVANLLGIPLGTYLSQEFSWRYTFLLIAVFNIVVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGN
AGVFAWFSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCGGMKITS
LIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSL
LLYGRYKRQQAADSPVLAKPLG
>Mature_421_residues
ASLVVILQAITLLATVISSRSGGCDGGMKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMISYYALGVVV
GAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSGFPHGAFFGVGAIVLSKIIKPGKVTAAVAGMV
SGMTVANLLGIPLGTYLSQEFSWRYTFLLIAVFNIVVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNA
GVFAWFSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCGGMKITSL
IFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLL
LYGRYKRQQAADSPVLAKPLG

Specific function: May be involved in either the transport or processing of arabinose polymers [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786595, Length=394, Percent_Identity=98.7309644670051, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI1787947, Length=379, Percent_Identity=32.1899736147757, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI145693198, Length=348, Percent_Identity=25.2873563218391, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787808, Length=259, Percent_Identity=25.8687258687259, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44650; Mature: 44519

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00027 HOMEOBOX_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure 
ASLVVILQAITLLATVISSRSGGCDGGMKKVILSLALGTFGLGMAEFGIMGVLTELAHN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1744033; 9278503 [H]