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The map label for this gene is napD [H]

Identifier: 170683051

GI number: 170683051

Start: 2387035

End: 2387298

Strand: Reverse

Name: napD [H]

Synonym: EcSMS35_2355

Alternate gene names: 170683051

Gene position: 2387298-2387035 (Counterclockwise)

Preceding gene: 170682644

Following gene: 170679943

Centisome position: 47.1

GC content: 54.17

Gene sequence:

>264_bases
ATGCACACTAACTGGCAAGTTTGCAGCCTGGTCGTGCAGGCCAAAAGCGAACGAATTTCAGACATCAGCACCCAACTGAA
CGCCTTTACCGGCTGTGAAGTTGCTGTCAGCGACGCGCCGAGCGGTCAGTTGATTGTGGTGGTGGAAGCAGAAGACAGCG
AAACGCTGATCCAAACCATTGAGTCAGTACGCAACGTAGAGGGCGTGCTGGCGGTGTCGCTGGTTTATCACCAGCAGGAA
GAGCAAGGTGAGGAAACACCATGA

Upstream 100 bases:

>100_bases
TCCGGGATCTACCAGCCGCAACTTAATAGCCAACTTTGCAACGGATGTGGAGCATGTGCAGCCAGCTGTCCGGTATCAGC
CATAACTGCGGAGTATCTCC

Downstream 100 bases:

>100_bases
AACTCAGTCGTCGTAGCTTTATGAAAGCTAACGCCGTTGCGGCCGCTGCGGCGGCTGCCGGTCTCAGCGTGCCGGGCGTT
GCCCGCGCCGTTGTTGGTCA

Product: assembly protein for periplasmic nitrate reductase

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQE
EQGEETP

Sequences:

>Translated_87_residues
MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQE
EQGEETP
>Mature_87_residues
MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQE
EQGEETP

Specific function: Plays a role in the correct assembly of subunits of the periplasmic napAB enzyme [H]

COG id: COG3062

COG function: function code P; Uncharacterized protein involved in formation of periplasmic nitrate reductase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788535, Length=87, Percent_Identity=98.8505747126437, Blast_Score=172, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005623 [H]

Pfam domain/function: PF03927 NapD [H]

EC number: NA

Molecular weight: Translated: 9473; Mature: 9473

Theoretical pI: Translated: 3.85; Mature: 3.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQLIVVVEAEDSETLIQTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHH
ESVRNVEGVLAVSLVYHQQEEQGEETP
HHHHHHHHHHHHHHHHHCCHHCCCCCC
>Mature Secondary Structure
MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQLIVVVEAEDSETLIQTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHH
ESVRNVEGVLAVSLVYHQQEEQGEETP
HHHHHHHHHHHHHHHHHCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]