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The map label for this gene is emrK [H]

Identifier: 170682886

GI number: 170682886

Start: 2572996

End: 2574159

Strand: Reverse

Name: emrK [H]

Synonym: EcSMS35_2518

Alternate gene names: 170682886

Gene position: 2574159-2572996 (Counterclockwise)

Preceding gene: 170680633

Following gene: 170682814

Centisome position: 50.79

GC content: 41.92

Gene sequence:

>1164_bases
GTGGAACAGATTAATTCAAATAAAAAACATTCTGACAGAAAAAAATATTTTGCTTTGTTAGCGGTAGTTTTATTTATTGC
GTTTTCAGGTGCCTATGCCTATTGGTCAATGGAATTAGAAGACATGATTAGTACAGATGACGCCTATGTCACGGGAAATG
CAGATCCAATTTCTGCACAAGTTTCAGGCAGCGTCACTGTCGTTAATCATAAAGATACGAACTACGTTCGACAGGGTGAC
ATTTTAGTCTCACTGGATAAGACTGATGCCACTATCGCACTCAATAAAGCTAAAAATAATCTGGCCAATATTGTTCGGCA
AACGAATAAACTGTACTTACAGGATAAACAATACAGTGCCGAAGTCGCTTCAGCTCGTATTCAGTATCAACAATCTTTAG
AAGATTATAACCGTCGTGTACCTTTAGCGAAGCAGGGGGTTATTTCAAAAGAAACGCTGGAGCATACCAAAGATACGTTA
ATAAGTAGCAAAGCGGCATTGAATGCTGCTATTCAGGCTTATAAAGCGAATAAAGCTTTAGTAATGAACACACCATTAAA
CCGTCAGCCACAAGTCGTTGAAGCGGCGGATGCAACCAAAGAAGCCTGGTTGGCGCTTAAACGTACGGATATTAAGAGTC
CGGTTACCGGCTATATTGCCCAGCGAAGTGTTCAGGTCGGCGAAACAGTGAGTCCCGGACAATCGTTAATGGCTGTCGTA
CCGGCACGTCAAATGTGGGTTAATGCCAACTTTAAAGAAACACAACTCACGGATGTGCGGATTGGTCAATCGGTCAATAT
TATTAGCGATCTTTATGGTGAAAATGTTGTGTTTCATGGTCGGGTGACAGGGATCAATATGGGAACCGGCAATGCGTTCT
CCTTATTACCTGCACAAAATGCGACAGGGAATTGGATCAAAATCGTTCAGCGTGTACCGGTTGAGGTTTCTCTTGATCCA
AAAGAACTCATGGAACATCCCTTGCGCATTGGTTTATCGATGACTGCAACTATTGATACCAGGAACGAAGACATTGCCGA
TATGCCCGATCTGGCTTCAACCGTGACGTCCATGCCTGCTTATACCAGTAAGGCTTTAATTATCGATACCAGTCCGATAG
AAAAAGAAATTAGCAACATTATTTCGCATAATGGACAACTTTAA

Upstream 100 bases:

>100_bases
CCTGGGATAATGTGCAACACATGCACTGTGTTTGATATGAAGAATGAATACTCTTTTCATTCAATTCATAAATTTCATCT
ATGCGAAATGAGAGATAAAA

Downstream 100 bases:

>100_bases
TGGCAATCACTAAATCAACTCCGGCACCATTAACCGGTGGGACGTTATGGTGCGTTACTATTGCATTGTCATTAGCGACG
TTTATGCAAATGTTGGATTC

Product: drug resistance MFS transporter, membrane fusion protein (MFP) subunit EmrK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQINSNKKHSDRKKYFALLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTRNEDIADMPDLASTVTSMPAYTSKALIIDTSPIEKEISNIISHNGQL

Sequences:

>Translated_387_residues
MEQINSNKKHSDRKKYFALLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTRNEDIADMPDLASTVTSMPAYTSKALIIDTSPIEKEISNIISHNGQL
>Mature_387_residues
MEQINSNKKHSDRKKYFALLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTRNEDIADMPDLASTVTSMPAYTSKALIIDTSPIEKEISNIISHNGQL

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=387, Percent_Identity=98.1912144702842, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1789041, Length=378, Percent_Identity=48.6772486772487, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1790519, Length=324, Percent_Identity=27.7777777777778, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=27.6527331189711, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789637, Length=310, Percent_Identity=26.1290322580645, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790024, Length=238, Percent_Identity=22.6890756302521, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42557; Mature: 42557

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQINSNKKHSDRKKYFALLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCEEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTRNEDIADMPDLASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
YTSKALIIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQINSNKKHSDRKKYFALLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCEEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTRNEDIADMPDLASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
YTSKALIIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]