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The map label for this gene is phnF [H]

Identifier: 170682760

GI number: 170682760

Start: 4667194

End: 4667919

Strand: Reverse

Name: phnF [H]

Synonym: EcSMS35_4568

Alternate gene names: 170682760

Gene position: 4667919-4667194 (Counterclockwise)

Preceding gene: 170680687

Following gene: 170682088

Centisome position: 92.1

GC content: 62.12

Gene sequence:

>726_bases
ATGCACTTGTCTACACATCCGACCAGCTACCCAACACGCTATCAGGAGATAGCCGCAAAACTTGAGCAGGAGCTTCGTCA
ACACTACCGCTGCGGCGACTATCTTCCCGCCGAGCAGCAACTGGCGGCGCGCTTTGAGGTGAACCGCCACACCCTGCGCC
GCGCCATCGACCAACTGGTGGAAAAAGGTTGGGTACAGCGCCGTCAGGGCGTCGGCGTGCTGGTGCTGATGCGCCCGTTC
GATTACCCGCTCAACGCCCAGGCGCGTTTTAGCCAGAACCTGCTGGACCAAGGCAGCCATCCCACCAGCGAAAAATTGCT
ATCGGTGCTGCGCCCCGCCTCCGGTCACGTCGCTGACGCATTGGGGATTACCGAGGGCGAGAACGTTATCCACCTGCGCA
CCCTGCGTCGGGTCAACGGCGTCGCGCTCTGTTTAATCGACCACTACTTTGCGGACCTCGCCCTCTGGCCGACGCTGCAA
CGCTTCGACAGCGGCTCGCTGCACGATTTTCTGCGCGAGCAAACCGGAATTGCGCTGCGCCGCAGCCAGACGCGGATCAG
CGCCCGCCGCGCCCAGGCCAAAGAGTGCCAGCGTCTTGAAATCCCGAATATGTCGCCGCTGCTGTGCGTGCGCACCCTTA
ACCACCGTGACGGTGAAAGCAGCCCGGCGGAGTACTCCGTCAGCCTGACGCGCGCCGACATGATTGAATTCACTATGGAG
CACTGA

Upstream 100 bases:

>100_bases
GCGCCCTGATGGTGCTGATTATCGTCACGGTCAGCCTGCTGGATTTCCTCTCTCAACGGTTGCGTAAGCACTTTATCTGA
TAAGCGAGGCATTGATATCT

Downstream 100 bases:

>100_bases
ATGCACGCAGATACCGCGACCCGCCAGCACTGGATGTCCGTACTGGCGCACAGCCAACCGACTGAGCTGGCAGCCCGCCT
GAACGCGCTGAACATCACCG

Product: phosphonate metabolism transcriptional regulator PhnF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPF
DYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
H

Sequences:

>Translated_241_residues
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPF
DYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
H
>Mature_241_residues
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPF
DYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
H

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known. By similarity could be a transcriptional regulator [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790540, Length=241, Percent_Identity=99.5850622406639, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1788418, Length=212, Percent_Identity=28.7735849056604, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790118, Length=231, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786950, Length=230, Percent_Identity=28.695652173913, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012702
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 27592; Mature: 27592

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
LGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQRFDSGSLHDFLREQTGIALR
HCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
RSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCC
H
C
>Mature Secondary Structure
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
LGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQRFDSGSLHDFLREQTGIALR
HCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
RSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]