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The map label for this gene is ttdR [H]

Identifier: 170682678

GI number: 170682678

Start: 3430842

End: 3431774

Strand: Reverse

Name: ttdR [H]

Synonym: EcSMS35_3353

Alternate gene names: 170682678

Gene position: 3431774-3430842 (Counterclockwise)

Preceding gene: 170682206

Following gene: 170683805

Centisome position: 67.71

GC content: 49.95

Gene sequence:

>933_bases
ATGCTGAATAGCTGGCCTTTAGCCAAAGATTTGCAGGTACTGGTAGAAATCGTCCACAGCGGGAGTTTTAGCGCCGCAGC
GGCGACGCTTGGGCAGACGCCTGCATTTGTCACCAAGCGGATCCAAATCCTTGAAAATACGCTGGCAACAACGCTGCTGA
ACCGCTCCGCTCGCGGCGTGGCGCTGACTGAAAGTGGCCAACGTTGTTACGAGCATGCGCTGGAAATCCTCACCCAATAT
CAGCGACTGGTCGATGACGTCACGCAAATCAAAACGCGCCCGGAGGGGATGATTCGTATTGGTTGCAGTTTTGGTTTTGG
GCGCAGCCATATTGCGCCAGCCATTACCGAACTGATGCGCAATTATCCTGAGTTACAGGTGCATTTTGAATTGTTCGATC
GGCAAATTGATTTAGTGCAGGATAATATTGATCTGGATATTCGTATTAATGACGAAATTCCTGATTATTATATTGCGCAT
CTGTTAACGAAAAATAAAAGAATATTATGCGCAGCACCTGAGTATCTGCAAAAATATCCGCAGCCACAATCCTTACAGGA
ATTAAGTCGTCATGATTGTCTGGTGACCAAAGAGCGCGATATGACCCACGGAATATGGGAGTTGGGGAACGGTCAGGAGA
AAAAATCGGTGAAGGTAAGCGGGCATCTTTCCTCCAATAGCGGCGAGATAGTGTTGCAATGGGCGCTGGAGGGCAAAGGG
ATTATGTTGCGTTCCGAGTGGGATGTGTTGCCGTTTCTGGAGAGTGGCAAACTGGTGCGAGTATTGCCGGAGTATGCACA
AAGCGCCAATATCTGGGCTGTTTACCGGGAGCCGCTCTATCGCAGCATGAAATTACGTGTCTGCGTGGAATTTCTGGCGG
CATGGTGCCAGCAACGGCTGGGCAAGCCCGACGAAGGCTATCAGGTCATGTAA

Upstream 100 bases:

>100_bases
GTGAATCAACGCGAAAATAAGAATGAATATTGCGCCAGAGCAGGGAATGGCAATGTTTTACGAAATACACTGCGCTTATC
ACAACTGCGGATAATTAACG

Downstream 100 bases:

>100_bases
ATCCACCAGAAATCACTCGGGATCCTTTTCGCGCTTTCTTTTGAATTTCGTCCAGATTTTTGTCTCCTGACGACGCCACA
GACGCTGGATGTTGTCATGA

Product: transcriptional activator TtdR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQILENTLATTLLNRSARGVALTESGQRCYEHALEILTQY
QRLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKG
IMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM

Sequences:

>Translated_310_residues
MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQILENTLATTLLNRSARGVALTESGQRCYEHALEILTQY
QRLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKG
IMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM
>Mature_310_residues
MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQILENTLATTLLNRSARGVALTESGQRCYEHALEILTQY
QRLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKG
IMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM

Specific function: Positive regulator required for L-tartrate-dependent anaerobic growth on glycerol. Induces expression of the ttdA-ttdB- ygjE operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789440, Length=310, Percent_Identity=99.6774193548387, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=48.7972508591065, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=28.1879194630872, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.1095890410959, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=26.3888888888889, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=24.1610738255034, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=23.8970588235294, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786448, Length=240, Percent_Identity=24.1666666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=166, Percent_Identity=28.3132530120482, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=183, Percent_Identity=28.4153005464481, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=266, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35343; Mature: 35343

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQILENTLATTLLNRSARGV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
ALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHH
NYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYP
CCCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHCC
QPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKG
CCHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCE
IMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
EEEECCCCCCCCCCCCCEEEECHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
GKPDEGYQVM
CCCCCCCCCC
>Mature Secondary Structure
MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQILENTLATTLLNRSARGV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
ALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHH
NYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYP
CCCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHCC
QPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKG
CCHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCE
IMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
EEEECCCCCCCCCCCCCEEEECHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
GKPDEGYQVM
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]