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The map label for this gene is hycF [H]

Identifier: 170682605

GI number: 170682605

Start: 2920102

End: 2920644

Strand: Reverse

Name: hycF [H]

Synonym: EcSMS35_2845

Alternate gene names: 170682605

Gene position: 2920644-2920102 (Counterclockwise)

Preceding gene: 170681957

Following gene: 170683663

Centisome position: 57.62

GC content: 55.43

Gene sequence:

>543_bases
ATGTTTACCTTTATCAAAAAAGTCATCAAAACCGGCACGGCGACCTCGTCTTATCCGCTGGAGCCGATTGCGGTTGATAA
AAACTTCCGTGGTAAGCCAGAGCAGAACCCGCAGCAGTGCATCGGCTGCTCGGCCTGCGTCAATGCCTGCCCGTCAAACG
CCTTAACGGTTGAAACTGACCTCGCCACAGGAGAGCTTGCCTGGGAGTTTAATCTCGGGCGCTGCATCTTCTGTGGACGC
TGCGAAGAAGTTTGCCCGACGGCGGCGATCAAACTGTCGCAAGAGTACGAACTGGCGGTGTGGAAGAAAGAAGACTTCCT
GCAACAGTCCCGCTTCGCGCTGTGCAACTGTCGCGTCTGCAATCGTCCTTTCGCCGTCCAGAAAGAGATCGACTACGCCA
TTGCGCTGCTTAAGCACAACGGCGACAGCCGCGCGGAAAACCACCGCGAAAGCTTTGAGACTTGCCCGGAATGTAAGCGC
CAGAAATGCCTGGTGCCGTCCGACCGTATTGAACTGACTCGCCATATGAAAGAGGCCATCTGA

Upstream 100 bases:

>100_bases
GGTCGATGTACGTAAGAAGAAGAGCAAAGTGGTGCCGTACAAAGAACTCGAGCGCTACAGCATTGAGCGTAAAAACTCGC
CGCTGAAATAAGGAATCGCC

Downstream 100 bases:

>100_bases
TGAGCAATTTATTAGGCCCCCGTGACGCTAACGGCATTCCGGTCCCGATGACGGTGGATGAATCCATCGCCAGCATGAAG
GCGTCGTTACTGAAAAAAAT

Product: formate hydrogenlyase complex iron-sulfur subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCSACVNACPSNALTVETDLATGELAWEFNLGRCIFCGR
CEEVCPTAAIKLSQEYELAVWKKEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
QKCLVPSDRIELTRHMKEAI

Sequences:

>Translated_180_residues
MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCSACVNACPSNALTVETDLATGELAWEFNLGRCIFCGR
CEEVCPTAAIKLSQEYELAVWKKEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
QKCLVPSDRIELTRHMKEAI
>Mature_180_residues
MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCSACVNACPSNALTVETDLATGELAWEFNLGRCIFCGR
CEEVCPTAAIKLSQEYELAVWKKEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
QKCLVPSDRIELTRHMKEAI

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789075, Length=180, Percent_Identity=98.8888888888889, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788833, Length=170, Percent_Identity=45.8823529411765, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1788617, Length=146, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20345; Mature: 20345

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
7.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCSACVNACPSNALTVETD
CHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
LATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWKKEDFLQQSRFALCNCRVC
CCCCEEEEEECCCEEEEECCCHHHCCHHHEECCCCCEEEEECHHHHHHHHHHHHHCCEEC
NRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKRQKCLVPSDRIELTRHMKEAI
CCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCSACVNACPSNALTVETD
CHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
LATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWKKEDFLQQSRFALCNCRVC
CCCCEEEEEECCCEEEEECCCHHHCCHHHEECCCCCEEEEECHHHHHHHHHHHHHCCEEC
NRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKRQKCLVPSDRIELTRHMKEAI
CCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]